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This document proposes labeling trehalose glycolipids in the cell wall of Mycobacterium tuberculosis with trehalose analogues containing azide groups. The azide-modified trehalose would bind to the glycolipids, and then bioorthogonal ligation would occur. Experiments are proposed to determine if the analogues label the glycolipids via the Ag85 pathway or recycling pathways by comparing labeling when the SugABCLpqY trehalose transporter is present or absent. The goal is to better understand the metabolism, reactions and biosynthesis of M. tuberculosis' complex cell wall.
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Biological role of peptidoglycans, glycasoaminoglycans, lectins.ppt
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EVALUATION OF ANOMERIC RECOGNITION IN GALACTOSE BINDING LECTINS USING.pdf
( Isi, 2016) international journal of biological macromolecules gst kappa 201...
( Isi, 2016) international journal of biological macromolecules gst kappa 201...
Effect of DX and Phosphorylation of Gal3-Binding Partner Interactions Draft 08
Effect of DX and Phosphorylation of Gal3-Binding Partner Interactions Draft 08
More from yanielisrodriguez
Curriculum vitae
Félix Vallés received a Bachelor's degree in Biology from the University of Puerto Rico at Cayey in 2017. He has research experience as a student in the RISE Program at UPR Cayey from 2013-2013, where he took courses in introduction to biology and scientific literature. Vallés has published papers on synthesis of thalidomide compounds and extra-pair paternity in birds that were presented at UPR Cayey. He is fluent in Spanish and English and is a member of the RISE Program and Jasperi Pre-vet Group.
Reflective essay
The RISE program at the University of Puerto Rico at Cayey helps undergraduate students develop scientific research skills. The student joined RISE in 2013 and took courses on scientific literature and writing techniques. These courses included seminars on various research topics as well as workshops on writing annotated bibliographies and review papers. While the student initially struggled with writing seminar reflections and bibliographies, their writing improved over time as they practiced. The main goal of the course was writing a review paper, which helped the student enhance their research and writing abilities. Similar programs at other universities also help students strengthen their scientific skills and prepare for research careers.
Reflective essay
The RISE program at the University of Puerto Rico at Cayey helps undergraduate students develop scientific research skills. The student joined RISE in 2013 and took courses on scientific literature and writing techniques. These courses included seminars, workshops, and assignments on writing annotated bibliographies and seminar reflections. Through these activities, the student improved their reading, writing, and comprehension abilities. A key activity was writing a review paper, which helped the student learn how to critically evaluate published information and improve their research skills. Similar university programs also help students enhance their scientific thinking and benefit from research opportunities that better prepare them for scientific careers.
Review paper rise.docx corrected
1. The document compares rates of extra-pair paternity (EPP) in three socially monogamous bird species: the mountain bluebird, little auk, and zebra finch.
2. Rates of EPP were found to be high (72% of broods) in mountain bluebirds, low (2% of copulations) in little auks, and varied between low rates in wild zebra finches (1.7% of offspring) versus high rates (28% of offspring) in captive zebra finches.
3. Traits like plumage ornamentation in male mountain bluebirds and songs in male zebra finches may increase reproductive success through EPP by
Review paper rise.docx corrected
The document compares rates of extra-pair paternity (EPP) in three socially monogamous bird species: the mountain bluebird, little auk, and zebra finch. EPP was found to be high in mountain bluebirds, with 72% of broods containing an extra-pair offspring. In little auks, over 60% of birds engaged in extra-pair copulation but only 2% of copulations resulted in offspring due to high rates of within-pair copulation. Rates of EPP in wild zebra finches were also low at 1.7% of offspring, whereas captive zebra finches showed much higher EPP. The document discusses how EPP can increase variation in male
4 seminar reflection wordpress
Central nervous system neurons are unable to regenerate unlike peripheral nervous system neurons due to inhibitory factors and low cAMP levels in the central nervous system. However, if peripheral and central nervous system branches are separated, the peripheral branch can cause cAMP levels to rise and allow the central branch to regrow. This research aims to develop a genetically manipulable model to identify molecules regulating axon regeneration. Caenorhabditis elegans is a suitable model as it has a simple nervous system with identifiable axon paths and synapses, as well as being small and transparent, and its genes, regeneration patterns, and mechanisms are similar to vertebrates.
3 seminar reflection wordpress
The seminar discussed research into the function of LPIN1 in the adrenal cortex. The presenter aimed to characterize LPIN1's function and how protein kinase A (PKA) regulates the enzyme diacylglycerol kinase (DGK). ACTH stimulates cortisol secretion through a signaling pathway involving cAMP, PKA, DGK, and other factors. LPIN1 is believed to convert phosphatidic acid (PA) into diacylglycerol (DAG) to reduce the activity of steroidogenic factor 1 (SF1) and thus influence cortisol production. The research sought to better understand LPIN1's role in this process.
2 seminar reflection wordpress
This seminar discussed Alan Turing and his work to help solve David Hilbert's Entscheidungsproblem. Turing proposed using machines to incorporate basic math in an attempt to settle Hilbert's problem of finding an algorithm that determines if mathematical statements are true or false. Turing invented the Turing machine, a finite program that uses symbols on tape according to rules. He concluded that Hilbert's problem was impossible to solve since the halting problem of determining if a program finishes or runs forever cannot be decided.
Propuesta rise thalidomide.ppt felix valles
This document presents a proposal to synthesize analogous compounds of thalidomide for inhibiting TNF-α without thalidomide's teratogenic effects. The proposal involves modifying thalidomide's structure through Michael additions and testing the new compounds for TNF-α inhibition, stereochemistry, viability, and presence of secondary products, as measured by assays like IC-50, ELISA, trypan blue, and HPLC. Similar or better TNF-α inhibition is expected compared to thalidomide due to retention of the inhibitory pharmacophore, without the enantiomer responsible for teratogenicity.
Propuesta rise
This document describes a study that aims to synthesize thalidomide analog compounds for inhibiting TNF-α without thalidomide's teratogenic effects. The analogs will be made by modifying thalidomide's 2-(2,6-dioxopiperidin-3-yl) group. They will be analyzed using HPLC, spectroscopy, and polarimetry. The analogs' functionality, efficiency, and viability will be tested against thalidomide using IC-50, ELISA, and Trypan Blue assays to measure TNF-α inhibition and toxicity. Similar or better inhibition than thalidomide is expected since the analogs retain the TNF-α inhibitory fragment, but lack the enantiomer that causes teratogenic
Review paper presentation rise.pptx
This document summarizes three studies that examine how extra-pair paternity increases variance in male reproductive success across different bird species. A study of mountain bluebirds found high rates of extra-pair copulation and paternity increased male reproductive success. In little auks, most extra-pair copulations were unsuccessful at fertilization despite being common. Zebra finches showed extremely low extra-pair paternity in the wild but high rates in captivity. Overall, extra-pair paternity increased variance in reproductive success for male mountain bluebirds, little auks engage in many unsuccessful extra-pair copulations, and rates differ between wild and captive zebra finches.
Review paper presentation rise.pptx
This document summarizes three studies on extra-pair paternity (EPP) in birds. A study of little auks found high rates of extra-pair copulation (EPC) but very low rates of EPP. Zebra finches in captivity showed high EPP unlike those in the wild, suggesting environmental factors influence EPP. Mountain bluebirds with more ornate plumage had higher EPP, potentially increasing sexual selection. Overall, the document finds EPP varies between species and can increase variance in male reproductive success.
Review paper presentation rise.pptx
This document summarizes three studies on extra-pair paternity (EPP) in birds. A study of little auks found high rates of extra-pair copulation (EPC) but very low rates of EPP. Zebra finches in captivity showed high EPP unlike those in the wild, suggesting environmental factors influence EPP. Mountain bluebirds with more ornate plumage had higher EPP, potentially increasing sexual selection. Overall, the studies found EPP can greatly vary between species and increase variance in male reproductive success.
Curriculim vitae
This document is a curriculum vitae for Yanielis Rodriguez Ramirez that includes personal information such as date of birth, education, and contact details. It describes her professional interests in pursuing a biology degree and a career in orthodontics or infectology. It also lists some professional experience in tutoring and participating in a science fair, as well as her academic preparation through a biology course in the RISE program that included presentations, tours, and a research proposal.
The dry forest of guanica and the caribbean
The document summarizes two field trips to different forest ecosystems in Puerto Rico - El Yunque National Forest and Guanica Dry Forest. It describes the location, climate, topography, flora, and fauna of each forest. It highlights differences between the forests, such as tree species composition and adaptations to climate. The document concludes that comparing the two forests provided insight into how plants evolve adaptations to their unique environments.
How do the moringa oleifera cure the constipation
This document proposes a study to determine if Moringa oleifera can cure constipation. The study would observe the effects of a Moringa oleifera treatment on constipated and non-constipated lab rats. The researcher hypothesizes that Moringa oleifera will not cure constipation in rats and may cause other symptoms. The methods section outlines how rats will be fed to induce constipation and how some rats in each group will receive Moringa oleifera treatment while others will act as controls. The expected results are that Moringa oleifera will not cure constipation and may cause side effects. Future studies could test Moringa oleifera's effects on other diseases.
Power point
En este documento se explica cómo resolver un cruce monohíbrido entre dos plantas de guisantes heterocigóticas para la característica de la forma de la semilla. Se indica que las semillas lisas (S) son dominantes sobre las semillas rugosas (s). El cruce entre dos plantas Aa producirá descendientes con un 50% de semillas lisas y un 50% de semillas rugosas.
Biologia humana 12
The immune system consists of lymphatic organs, antibodies, and regulatory agents that work together to defend the body against diseases. The primary lymphatic organs are the red bone marrow and thymus gland, where lymphocytes originate and mature. Lymphocytes then congregate in secondary lymphatic organs like the spleen, lymph nodes, and tonsils. When tissue rejection occurs, the immune system recognizes and destroys cells bearing foreign MHC antigens. Diseases of the immune system include autoimmune disorders, where the immune system mistakenly attacks the body's own cells, and immune deficiencies, where the immune system is unable to adequately protect against disease.
Trabajo para fisica de feria
La investigación buscó determinar si la bacteria Escherichia coli estaba presente en los carritos de compra de dos supermercados en Cayey examinando muestras tomadas de 10 carritos de cada supermercado. Basado en un análisis de laboratorio de las muestras, la hipótesis era que los carritos no estaban calificados para uso público debido a la presencia probable de E. coli.
Feria cientifica tiffany colon y yanielis rodriguez
El estudio analizó la presencia de bacterias como Escherichia coli en los carritos de dos supermercados en Cayey. Diez muestras fueron tomadas de cada supermercado y llevadas a un laboratorio. Cuatro de las muestras contenían bacterias, con dos del primer supermercado y dos del segundo conteniendo Escherichia coli u otras bacterias. El segundo supermercado tuvo más bacterias en general, confirmando la hipótesis de que los carritos podrían contener bacterias.
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Feria cientifica tiffany colon y yanielis rodriguez
Feria cientifica tiffany colon y yanielis rodriguez
1 seminar reflection wordpress
- 1. Félix J. Vallés F. Seminar 1 September 21, 2013 University of Puerto Rico – Cayey RISE Program Biol. 3095 Seminar Scientific Literature and Bibliography Mycobacterium tuberculosis contains a complex cell wall which provides a high resistance to antibiotics. This investigation states that trehalose glycolipids can be labeled with these trehalose analogues: 2-TreAZ, 3-TreAz, 4-TreAz or 6-TreAz. First, the azide-modified trehalose will bind totrehalose glycolipids, and then,bioorthogal ligation will occur in those glycolipids that were successfully labeled. The proposal consists of putting mycobacterium in solutions that contain these analogues and see whether they label the glycolipids via Ag85 or the recycling pathways. This process is done by putting the glycolipids in the solution with analogues, then repeating the same step but without the SugABCLpqY, which is the trehalose specific transporter. If the analogues in the solution bind to the glycolipids, the labeling is therefore via Ag85; if not, it occurs via recycling pathways. The purpose of this proposal is the better understanding of the metabolism, reactions and biosynthesis of the cell wall of this mycobacteria.