The document provides details for a team presentation on September 28th regarding a final project presentation format. It includes instructions to get customer feedback, presentation guidelines including a 2-minute video and 20-minute presentation, and notes on what the team thought and learned on different days as well as where they ended and what they plan to do next.
Challenges & Solutions for Online Lab Science CoursesPeter Jeschofnig
This document discusses challenges and solutions for online lab science courses. It presents results comparing student performance in traditional face-to-face chemistry courses with on-campus labs versus online courses using lab kits. Assessment results, including ACS standardized exams and lab report scores, showed no significant differences in learning outcomes between the two formats. The document also explores options for conducting online labs, including simulations, kitchen chemistry, assembled kits, and micro-scale techniques.
The document provides information about Science Prof Online (SPO), a free science education website that offers various educational resources like virtual classrooms, PowerPoints, articles and images. It details the types of materials available on SPO like practice questions, lecture slides, video tutorials and more. The document also provides guidance on how to access and use the PowerPoint slides available on SPO, such as downloading editable or non-editable versions. It concludes by crediting the chief creators of SPO and their contact information.
What can you learn from molecular modeling?digitalbio
A presentation given by Dr. Sandra Porter at the Biotechnology Educators Conference, Virginia Bioinformatics Institute, July 2015
For more information, see http://DigitalWorldBiology.com
This comics book was developped by the Labonfoil projet, a research project funded by the European Commission. I am not the author, just uploaded so that people can view this magnificent piece of science dissemination.
- The team developed a reproducible biochemical analysis approach for phage genome sequencing using a portable DNA sequencer called the MinION to make the approach affordable and accessible. They successfully tested their approach by sequencing a mono-phage sample from Ukraine and discovered a new phage genome.
- Moving forward, they plan to engage students through school projects using their approach, conduct mobile phage sequencing labs in rural areas using the portability of their method, and develop an online phage database to accelerate phage research.
This document provides an overview of genomic tools and best practices for scientific computing. It discusses SequenceServer, a tool for BLAST searches, and Bionode, a collection of Node.js modules for bioinformatics. It also discusses challenges with gene prediction and introduces GeneValidator, a tool for visual inspection and manual correction of gene predictions. Key points include automating repetitive tasks, writing code for people through style guides, and using version control and modularization to improve code quality and reproducibility.
Paulo Vilaça outlines his educational and professional background, starting with completing a BSc in Computer Science Engineering in 2007 and an MSc in Bioinformatics in 2009. In 2010, he co-founded SilicoLife with colleagues to design optimal cell factories using computer modeling. SilicoLife started as a garage startup and later moved to a business incubator park and then converted a house near the University of Minho, where it is currently located. The company uses genome-scale modeling to enable the design of microbial strains optimized for producing biofuels, chemicals, and biopolymers.
Challenges & Solutions for Online Lab Science CoursesPeter Jeschofnig
This document discusses challenges and solutions for online lab science courses. It presents results comparing student performance in traditional face-to-face chemistry courses with on-campus labs versus online courses using lab kits. Assessment results, including ACS standardized exams and lab report scores, showed no significant differences in learning outcomes between the two formats. The document also explores options for conducting online labs, including simulations, kitchen chemistry, assembled kits, and micro-scale techniques.
The document provides information about Science Prof Online (SPO), a free science education website that offers various educational resources like virtual classrooms, PowerPoints, articles and images. It details the types of materials available on SPO like practice questions, lecture slides, video tutorials and more. The document also provides guidance on how to access and use the PowerPoint slides available on SPO, such as downloading editable or non-editable versions. It concludes by crediting the chief creators of SPO and their contact information.
What can you learn from molecular modeling?digitalbio
A presentation given by Dr. Sandra Porter at the Biotechnology Educators Conference, Virginia Bioinformatics Institute, July 2015
For more information, see http://DigitalWorldBiology.com
This comics book was developped by the Labonfoil projet, a research project funded by the European Commission. I am not the author, just uploaded so that people can view this magnificent piece of science dissemination.
- The team developed a reproducible biochemical analysis approach for phage genome sequencing using a portable DNA sequencer called the MinION to make the approach affordable and accessible. They successfully tested their approach by sequencing a mono-phage sample from Ukraine and discovered a new phage genome.
- Moving forward, they plan to engage students through school projects using their approach, conduct mobile phage sequencing labs in rural areas using the portability of their method, and develop an online phage database to accelerate phage research.
This document provides an overview of genomic tools and best practices for scientific computing. It discusses SequenceServer, a tool for BLAST searches, and Bionode, a collection of Node.js modules for bioinformatics. It also discusses challenges with gene prediction and introduces GeneValidator, a tool for visual inspection and manual correction of gene predictions. Key points include automating repetitive tasks, writing code for people through style guides, and using version control and modularization to improve code quality and reproducibility.
Paulo Vilaça outlines his educational and professional background, starting with completing a BSc in Computer Science Engineering in 2007 and an MSc in Bioinformatics in 2009. In 2010, he co-founded SilicoLife with colleagues to design optimal cell factories using computer modeling. SilicoLife started as a garage startup and later moved to a business incubator park and then converted a house near the University of Minho, where it is currently located. The company uses genome-scale modeling to enable the design of microbial strains optimized for producing biofuels, chemicals, and biopolymers.
This document outlines the modules of a scientific writing workshop. Module 7 focuses on language and discusses concepts like plain English and proper use of prepositions when writing in English. It provides examples of needlessly complex words to avoid, words that are commonly misused, and verbs that take certain prepositions. The document emphasizes writing clearly and concisely in English for scientific publications.
The document summarizes the 2012 iGEM competition project from Carnegie Mellon University. The team developed fluorescent biosensors to characterize promoters by tagging mRNA with Spinach and proteins with a fluorogen activating protein (FAP). They created new inducible promoters and used fluorescence measurements and a mathematical model to characterize transcription and translation rates. The goal was to provide a better way to measure cellular activity without disrupting cells.
This document provides an overview of a book on physical optics and its applications. It discusses the book's unique approach of presenting both theoretical and practical aspects of each topic. The book covers waves, interference, diffraction gratings, interferometers, and spectrometers over seven chapters. It includes 222 figures, 19 tables, and 22 experimental cases. The book aims to provide both students and professionals a solid foundation in physical optics and hands-on experience through its experimental sections.
This document provides an introduction to the topic of waves. It discusses the nature of waves, including how they transfer energy and the types of mechanical waves. It describes the characteristics of waves such as transverse waves, longitudinal waves, and water waves. The document also covers simple harmonic motion, the period and frequency of waves, and the simple pendulum. It concludes by outlining three experimental cases to demonstrate Hook's law for springs, generating different types of waves, and measuring the period of a simple pendulum.
My placement 2022/2023 at Sitran with RNA bio LAb under Prof. Guillaume Hautb...md4afxz
1) The author completed their placement year at the Sheffield Institute for Translational Neuroscience working in the RNA Lab under the supervision of Professor Guillaume Hautbergue and Dr. Ya-Hui Lin.
2) Their role involved maintaining lab disposables and buffers as a research assistant and learning key lab techniques in molecular biology and protein analysis.
3) They found the placement year to be an invaluable experience for their personal and professional growth that allowed them to gain practical skills and develop their passion for neuroscience research.
nitzipper® is a new bioconjugation technology developed by nanoimmunotech that allows for the simple, quick, and versatile conjugation of various molecules and particles. It involves complementary linker molecules (Linker U and Linker T) that enable the oriented and stable conjugation of different components in a single step. Nitzipper® provides advantages over other conjugation methods like reduced time and cost, greater versatility in possible conjugations, and maintenance of biomolecule orientation and activity. It can be used for applications like immunoassays, flow cytometry, biosensing, and developing new customized conjugates and applications.
Here are the steps for Experiment #2:
1. Prepare a saline solution by dissolving 1 teaspoon of NaCl in 100 ml of distilled water in the beaker.
2. Place the baby diaper on a sheet of filter paper.
3. Use the graduated cylinder to pour 100 ml of the saline solution onto the center of the diaper.
4. Observe how quickly the liquid is absorbed and spreads out. Note the distribution of moisture.
5. Wait 15 minutes then repeat the procedure, observing if the liquid is absorbed as quickly and how wet the diaper is.
6. Carefully tear apart the diaper and try to identify its components like the absorbent polymers.
Nanotechnology involves manipulating matter at the atomic or nano scale. The document outlines the history, definitions, and approaches to nanotechnology such as top-down and bottom-up. It discusses applications of nanotechnology in food processing and packaging by using nano capsules for delivery and improving shelf life. Other applications mentioned include optics, medicine, energy, and more. The conclusion states that nanotechnology is an emerging field that will transform industries and potentially help address challenges in health, energy, and beyond.
Being a Postdoc (Sometimes): My Career Under The MicroscopeChris Willmott
Jonathan Howe has had a career in biological research, beginning with a BSc in Medical Biochemistry from the University of Leicester. He then completed a PhD there on cell migration and fibronectin fibrillogenesis. After working as a senior research associate at the University of East Anglia, he became a Career Development Fellow at the prestigious MRC Laboratory of Molecular Biology. As a postdoc there, his responsibilities included conducting research, supervising students, publishing papers, teaching, and maintaining the light microscopy facility. He notes that becoming an independent researcher is highly competitive and requires a strong publication record, while remaining a postdoc long-term offers less job security. Alternative career paths for postdocs include specializing in a technique
The document discusses the use of visualizations to analyze large datasets of bacterial gene sequences and neighborhoods in order to help experts identify patterns and functions. It outlines the development of "BactoGeNIE", a new visualization approach created by the author to scale to big data and large displays for the purpose of comparative bacterial gene neighborhood analysis. The visualization is intended to bring experts into the process of exploring gene neighborhoods across many genomes to gain insights that could not be found through automated methods alone.
Ingenza Ltd is an industrial biotechnology company in Edinburgh, UK that provides synthetic biology and bioprocess development services to chemical, therapeutics, and academic customers. It has capabilities in strain construction, protein engineering, fermentation up to 2L scale, and process development. A key technology is its proprietary inABLE assembly method that allows combinatorial assembly of up to 10 DNA fragments in a single reaction. Ingenza has applied inABLE and screening techniques to optimize pathways and expression for clients. It also operates cGMP compliant facilities for production of biologics in microbial and mammalian hosts.
BIpredict: MOE/web Server Enabled Delivery of In Silico Properties and ModelsDavid Thompson
Presentation at the Chemical Computing Group UGM in 2011. I describe my use of the MOE/web SOAP server and the model of delivering physico-chemical properties onto the medicinal chemists desktop.
The document discusses nanoparticles and carbon nano tubes. It summarizes topics from a workshop including assessing the environmental aspects and latest research on nanoparticles, establishing material safety standards for producers and consumers, and properties of nanoparticles less than 100nm. It notes nanoparticles have different properties than bulk materials and discusses exposure concerns from manufacturing, the environment, and public health. It stresses the need to determine nanoparticle chemistry, physics, and biology to understand uptake and effects, and establish standards while considering exposure levels during risk assessments.
This document summarizes Jillian Aurisano's master's thesis defense presentation on developing a scalable genome visualization tool called BactoGeNIE for comparative bacterial gene neighborhood analysis. It discusses how the sequencing of thousands of bacterial genomes has generated big data that is difficult for experts to analyze without specialized visualization tools. BactoGeNIE was designed to scale to large datasets and displays to enable experts to explore gene neighborhoods across genomes and gain insights that may not be found through automated methods alone.
The document discusses using visualizations of bacterial gene neighborhoods to help computational biologists explore large datasets of bacterial genome sequences and identify patterns and functions of unknown genes. It describes how comparing gene neighborhoods across genomes can provide insights into gene function, but that existing visualization approaches may not scale well to big data. The author's thesis presents a new visualization called BactoGeNIE designed for comparative gene neighborhood analysis that can scale to handle large genome datasets and be viewed on big displays.
The document discusses using comparative gene neighborhood analysis and visualization to help understand bacterial gene function from large genome sequence datasets. It describes how genes involved in similar biological processes are often located near each other in bacterial genomes. By comparing gene neighborhoods across different genomes, functions can be predicted for unknown genes. However, this requires analyzing many gene neighborhoods to identify statistically significant patterns. The author's thesis examines designing a new visualization called BactoGeNIE that can scale to "big data" sizes and large displays to enable experts to explore and analyze comparative gene neighborhood data in an interactive way.
Revised Custom Kanji Practice Sheets LearnJapKatie Parker
The document discusses the benefits of requiring students to take a personal finance course before graduating high school. It argues that many students lack knowledge of managing money, which prevents them from developing important financial skills. Without these skills, students are unprepared to budget their income effectively when entering the workforce, which can lead to debt problems. Requiring a personal finance course could help students learn how to budget and reduce debt.
Genome assembly: the art of trying to make one big thing from millions of ver...Keith Bradnam
A talk about genome assembly. Largely aimed at people new to the field, this slide deck is an updated version of a talk that I first gave last year and which I recently presented as part of a UC Davis Bioinformatics Core training workshop.
Author: Keith Bradnam, Genome Center, UC Davis
This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License.
We present our implementation and our reflections on a preregistration-based publication process for the fuzzing community with a pre-stage in the FUZZING workshop (https://fuzzingworkshop.github.io/), plus Stage 1 and Stage 2 at ACM Transactions of Software Engineering and Methodology (TOSEM; https://dl.acm.org/journal/tosem/registered-papers ).
Team Networks - 2022 Technology, Innovation & Great Power CompetitionStanford University
Technology Innovation and Great Power Competition,TIGPC, Gordian knot Center, DIME-FIL, department of defense, dod, intlpol 340, joe felter, ms&e296, raj shah, stanford, Steve blank, AI, ML, AI/ML, china, networks
Team LiOn Batteries - 2022 Technology, Innovation & Great Power CompetitionStanford University
Technology Innovation and Great Power Competition,TIGPC, Gordian knot Center, DIME-FIL, department of defense, dod, intlpol 340, joe felter, ms&e296, raj shah, stanford, Steve blank, AI, ML, AI/ML, china, LiOn Batteries
This document outlines the modules of a scientific writing workshop. Module 7 focuses on language and discusses concepts like plain English and proper use of prepositions when writing in English. It provides examples of needlessly complex words to avoid, words that are commonly misused, and verbs that take certain prepositions. The document emphasizes writing clearly and concisely in English for scientific publications.
The document summarizes the 2012 iGEM competition project from Carnegie Mellon University. The team developed fluorescent biosensors to characterize promoters by tagging mRNA with Spinach and proteins with a fluorogen activating protein (FAP). They created new inducible promoters and used fluorescence measurements and a mathematical model to characterize transcription and translation rates. The goal was to provide a better way to measure cellular activity without disrupting cells.
This document provides an overview of a book on physical optics and its applications. It discusses the book's unique approach of presenting both theoretical and practical aspects of each topic. The book covers waves, interference, diffraction gratings, interferometers, and spectrometers over seven chapters. It includes 222 figures, 19 tables, and 22 experimental cases. The book aims to provide both students and professionals a solid foundation in physical optics and hands-on experience through its experimental sections.
This document provides an introduction to the topic of waves. It discusses the nature of waves, including how they transfer energy and the types of mechanical waves. It describes the characteristics of waves such as transverse waves, longitudinal waves, and water waves. The document also covers simple harmonic motion, the period and frequency of waves, and the simple pendulum. It concludes by outlining three experimental cases to demonstrate Hook's law for springs, generating different types of waves, and measuring the period of a simple pendulum.
My placement 2022/2023 at Sitran with RNA bio LAb under Prof. Guillaume Hautb...md4afxz
1) The author completed their placement year at the Sheffield Institute for Translational Neuroscience working in the RNA Lab under the supervision of Professor Guillaume Hautbergue and Dr. Ya-Hui Lin.
2) Their role involved maintaining lab disposables and buffers as a research assistant and learning key lab techniques in molecular biology and protein analysis.
3) They found the placement year to be an invaluable experience for their personal and professional growth that allowed them to gain practical skills and develop their passion for neuroscience research.
nitzipper® is a new bioconjugation technology developed by nanoimmunotech that allows for the simple, quick, and versatile conjugation of various molecules and particles. It involves complementary linker molecules (Linker U and Linker T) that enable the oriented and stable conjugation of different components in a single step. Nitzipper® provides advantages over other conjugation methods like reduced time and cost, greater versatility in possible conjugations, and maintenance of biomolecule orientation and activity. It can be used for applications like immunoassays, flow cytometry, biosensing, and developing new customized conjugates and applications.
Here are the steps for Experiment #2:
1. Prepare a saline solution by dissolving 1 teaspoon of NaCl in 100 ml of distilled water in the beaker.
2. Place the baby diaper on a sheet of filter paper.
3. Use the graduated cylinder to pour 100 ml of the saline solution onto the center of the diaper.
4. Observe how quickly the liquid is absorbed and spreads out. Note the distribution of moisture.
5. Wait 15 minutes then repeat the procedure, observing if the liquid is absorbed as quickly and how wet the diaper is.
6. Carefully tear apart the diaper and try to identify its components like the absorbent polymers.
Nanotechnology involves manipulating matter at the atomic or nano scale. The document outlines the history, definitions, and approaches to nanotechnology such as top-down and bottom-up. It discusses applications of nanotechnology in food processing and packaging by using nano capsules for delivery and improving shelf life. Other applications mentioned include optics, medicine, energy, and more. The conclusion states that nanotechnology is an emerging field that will transform industries and potentially help address challenges in health, energy, and beyond.
Being a Postdoc (Sometimes): My Career Under The MicroscopeChris Willmott
Jonathan Howe has had a career in biological research, beginning with a BSc in Medical Biochemistry from the University of Leicester. He then completed a PhD there on cell migration and fibronectin fibrillogenesis. After working as a senior research associate at the University of East Anglia, he became a Career Development Fellow at the prestigious MRC Laboratory of Molecular Biology. As a postdoc there, his responsibilities included conducting research, supervising students, publishing papers, teaching, and maintaining the light microscopy facility. He notes that becoming an independent researcher is highly competitive and requires a strong publication record, while remaining a postdoc long-term offers less job security. Alternative career paths for postdocs include specializing in a technique
The document discusses the use of visualizations to analyze large datasets of bacterial gene sequences and neighborhoods in order to help experts identify patterns and functions. It outlines the development of "BactoGeNIE", a new visualization approach created by the author to scale to big data and large displays for the purpose of comparative bacterial gene neighborhood analysis. The visualization is intended to bring experts into the process of exploring gene neighborhoods across many genomes to gain insights that could not be found through automated methods alone.
Ingenza Ltd is an industrial biotechnology company in Edinburgh, UK that provides synthetic biology and bioprocess development services to chemical, therapeutics, and academic customers. It has capabilities in strain construction, protein engineering, fermentation up to 2L scale, and process development. A key technology is its proprietary inABLE assembly method that allows combinatorial assembly of up to 10 DNA fragments in a single reaction. Ingenza has applied inABLE and screening techniques to optimize pathways and expression for clients. It also operates cGMP compliant facilities for production of biologics in microbial and mammalian hosts.
BIpredict: MOE/web Server Enabled Delivery of In Silico Properties and ModelsDavid Thompson
Presentation at the Chemical Computing Group UGM in 2011. I describe my use of the MOE/web SOAP server and the model of delivering physico-chemical properties onto the medicinal chemists desktop.
The document discusses nanoparticles and carbon nano tubes. It summarizes topics from a workshop including assessing the environmental aspects and latest research on nanoparticles, establishing material safety standards for producers and consumers, and properties of nanoparticles less than 100nm. It notes nanoparticles have different properties than bulk materials and discusses exposure concerns from manufacturing, the environment, and public health. It stresses the need to determine nanoparticle chemistry, physics, and biology to understand uptake and effects, and establish standards while considering exposure levels during risk assessments.
This document summarizes Jillian Aurisano's master's thesis defense presentation on developing a scalable genome visualization tool called BactoGeNIE for comparative bacterial gene neighborhood analysis. It discusses how the sequencing of thousands of bacterial genomes has generated big data that is difficult for experts to analyze without specialized visualization tools. BactoGeNIE was designed to scale to large datasets and displays to enable experts to explore gene neighborhoods across genomes and gain insights that may not be found through automated methods alone.
The document discusses using visualizations of bacterial gene neighborhoods to help computational biologists explore large datasets of bacterial genome sequences and identify patterns and functions of unknown genes. It describes how comparing gene neighborhoods across genomes can provide insights into gene function, but that existing visualization approaches may not scale well to big data. The author's thesis presents a new visualization called BactoGeNIE designed for comparative gene neighborhood analysis that can scale to handle large genome datasets and be viewed on big displays.
The document discusses using comparative gene neighborhood analysis and visualization to help understand bacterial gene function from large genome sequence datasets. It describes how genes involved in similar biological processes are often located near each other in bacterial genomes. By comparing gene neighborhoods across different genomes, functions can be predicted for unknown genes. However, this requires analyzing many gene neighborhoods to identify statistically significant patterns. The author's thesis examines designing a new visualization called BactoGeNIE that can scale to "big data" sizes and large displays to enable experts to explore and analyze comparative gene neighborhood data in an interactive way.
Revised Custom Kanji Practice Sheets LearnJapKatie Parker
The document discusses the benefits of requiring students to take a personal finance course before graduating high school. It argues that many students lack knowledge of managing money, which prevents them from developing important financial skills. Without these skills, students are unprepared to budget their income effectively when entering the workforce, which can lead to debt problems. Requiring a personal finance course could help students learn how to budget and reduce debt.
Genome assembly: the art of trying to make one big thing from millions of ver...Keith Bradnam
A talk about genome assembly. Largely aimed at people new to the field, this slide deck is an updated version of a talk that I first gave last year and which I recently presented as part of a UC Davis Bioinformatics Core training workshop.
Author: Keith Bradnam, Genome Center, UC Davis
This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License.
We present our implementation and our reflections on a preregistration-based publication process for the fuzzing community with a pre-stage in the FUZZING workshop (https://fuzzingworkshop.github.io/), plus Stage 1 and Stage 2 at ACM Transactions of Software Engineering and Methodology (TOSEM; https://dl.acm.org/journal/tosem/registered-papers ).
Team Networks - 2022 Technology, Innovation & Great Power CompetitionStanford University
Technology Innovation and Great Power Competition,TIGPC, Gordian knot Center, DIME-FIL, department of defense, dod, intlpol 340, joe felter, ms&e296, raj shah, stanford, Steve blank, AI, ML, AI/ML, china, networks
Team LiOn Batteries - 2022 Technology, Innovation & Great Power CompetitionStanford University
Technology Innovation and Great Power Competition,TIGPC, Gordian knot Center, DIME-FIL, department of defense, dod, intlpol 340, joe felter, ms&e296, raj shah, stanford, Steve blank, AI, ML, AI/ML, china, LiOn Batteries
Team Quantum - 2022 Technology, Innovation & Great Power CompetitionStanford University
Technology Innovation and Great Power Competition,TIGPC, Gordian knot Center, DIME-FIL, department of defense, dod, intlpol 340, joe felter, ms&e296, raj shah, stanford, Steve blank, AI, ML, AI/ML, china, Quantum
Team Disinformation - 2022 Technology, Innovation & Great Power CompetitionStanford University
Technology Innovation and Great Power Competition,TIGPC, Gordian knot Center, DIME-FIL, department of defense, dod, intlpol 340, joe felter, ms&e296, raj shah, stanford, Steve blank, AI, ML, AI/ML, china, Disinformation
Team Wargames - 2022 Technology, Innovation & Great Power CompetitionStanford University
Technology Innovation and Great Power Competition,TIGPC, Gordian knot Center, DIME-FIL, department of defense, dod, intlpol 340, joe felter, ms&e296, raj shah, stanford, Steve blank, AI, ML, AI/ML, china, Wargames
Team Acquistion - 2022 Technology, Innovation & Great Power Competition Stanford University
Technology Innovation and Great Power Competition,TIGPC, Gordian knot Center, DIME-FIL, department of defense, dod, intlpol 340, joe felter, ms&e296, raj shah, stanford, Steve blank, AI, ML, AI/ML, china, Acquistion
Team Climate Change - 2022 Technology, Innovation & Great Power Competition Stanford University
Technology Innovation and Great Power Competition,TIGPC, Gordian knot Center, DIME-FIL, department of defense, dod, intlpol 340, joe felter, ms&e296, raj shah, stanford, Steve blank, AI, ML, AI/ML, china, climate
The document describes a team's efforts to commercialize a new protein quantification technology called PLA-Seq. After initially thinking the technology's value propositions of lower cost, faster throughput, and lower sample volume would appeal to pharmaceutical and personalized health companies, the team conducted customer interviews and learned accuracy was more important than cost to most customers. They also found their target markets should be preclinical biotech and academia rather than personalized health or CROs. The team incorporated their business and pivoted their marketing strategy and funding plans accordingly based on learnings outside of the building.
The document summarizes the development of Invisa Bio over 10 weeks as they pivoted between different medical applications and solutions for their self-assembling medical device technology. They initially focused on manufacturing and delivery but shifted to leveraging drug delivery mechanisms. They considered applications in cardiology, neurology, and orthopedics before focusing on brain aneurysms based on feedback from physicians. The company incorporated, raised funding, and began shadowing doctors to further develop their technology to address unmet needs in difficult to reach areas.
(1) The document describes the journey of a team developing a saffron supplement product to address mental health issues like anxiety and depression.
(2) It started with the goal of targeting adults aged 18-40, but through customer interviews and testing, they learned that teenagers were more interested in an anti-anxiety gummy product.
(3) Key lessons included the challenges of building the right team, navigating advice, knowing when enough customer feedback has been received, and setting individual and project milestones. The team is now continuing work over the summer to further develop the product.
Team Army venture capital - 2021 Technology, Innovation & Great Power Competi...Stanford University
Technology, Innovation and Great Power Competition,TIGPC, Gordian knot Center, DIME-FIL, department of defense, dod, intlpol 340, joe felter, ms&e296, raj shah, stanford, Steve blank, AI, ML, AI/ML, china, unmanned, autonomy, Army venture capital
Team Army venture capital - 2021 Technology, Innovation & Great Power Competi...Stanford University
Technology, Innovation and Great Power Competition,TIGPC, Gordian knot Center, DIME-FIL, department of defense, dod, intlpol 340, joe felter, ms&e296, raj shah, stanford, Steve Blank, Army Venture capital
Team Catena - 2021 Technology, Innovation & Great Power CompetitionStanford University
Technology, Innovation and Great Power Competition,TIGPC, Gordian knot Center, DIME-FIL, department of defense, dod, intlpol 340, joe felter, ms&e296, raj shah, stanford, Steve blank, AI, ML, AI/ML, china, unmanned, autonomy, economic coercion,
Team Apollo - 2021 Technology, Innovation & Great Power CompetitionStanford University
Technology, Innovation and Great Power Competition,TIGPC, Gordian knot Center, DIME-FIL, department of defense, dod, intlpol 340, joe felter, ms&e296, raj shah, stanford, Steve blank, AI, ML, AI/ML, china, unmanned, autonomy, space force
Team Drone - 2021 Technology, Innovation & Great Power CompetitionStanford University
Technology, Innovation and Great Power Competition,TIGPC, Gordian knot Center, DIME-FIL, department of defense, dod, intlpol 340, joe felter, ms&e296, raj shah, stanford, Steve blank, AI, ML, AI/ML, china, unmanned, autonomy, c3i, command and control
Team Short Circuit - 2021 Technology, Innovation & Great Power CompetitionStanford University
Technology, Innovation and Great Power Competition,TIGPC, Gordian knot Center, DIME-FIL, department of defense, dod, intlpol 340, joe felter, ms&e296, raj shah, stanford, Steve blank, AI, ML, AI/ML, china, unmanned, autonomy, semiconductors
Team Aurora - 2021 Technology, Innovation & Great Power CompetitionStanford University
Technology, Innovation and Great Power Competition,TIGPC, Gordian knot Center, DIME-FIL, department of defense, dod, intlpol 340, joe felter, ms&e296, raj shah, stanford, Steve blank, AI, ML, AI/ML, china, unmanned, autonomy, Army venture capital
Team Conflicted Capital Team - 2021 Technology, Innovation & Great Power Comp...Stanford University
Technology, Innovation and Great Power Competition,TIGPC, Gordian knot Center, DIME-FIL, department of defense, dod, intlpol 340, joe felter, ms&e296, raj shah, stanford, Steve blank, AI, ML, AI/ML, china, unmanned, autonomy, venture capital
Lecture 8 - Technology, Innovation and Great Power Competition - CyberStanford University
Technology, Innovation and Great Power Competition,TIGPC, Gordian knot Center, DIME-FIL, department of defense, dod, hacking for defense, intlpol 340, joe felter, ms&e296, raj shah, stanford, Steve blank, AI, ML, AI/ML, china, unmanned, autonomy, Michael Sulmeyer, cybercom,USCYBERCOM
How to Setup Warehouse & Location in Odoo 17 InventoryCeline George
In this slide, we'll explore how to set up warehouses and locations in Odoo 17 Inventory. This will help us manage our stock effectively, track inventory levels, and streamline warehouse operations.
How to Manage Your Lost Opportunities in Odoo 17 CRMCeline George
Odoo 17 CRM allows us to track why we lose sales opportunities with "Lost Reasons." This helps analyze our sales process and identify areas for improvement. Here's how to configure lost reasons in Odoo 17 CRM
Main Java[All of the Base Concepts}.docxadhitya5119
This is part 1 of my Java Learning Journey. This Contains Custom methods, classes, constructors, packages, multithreading , try- catch block, finally block and more.
LAND USE LAND COVER AND NDVI OF MIRZAPUR DISTRICT, UPRAHUL
This Dissertation explores the particular circumstances of Mirzapur, a region located in the
core of India. Mirzapur, with its varied terrains and abundant biodiversity, offers an optimal
environment for investigating the changes in vegetation cover dynamics. Our study utilizes
advanced technologies such as GIS (Geographic Information Systems) and Remote sensing to
analyze the transformations that have taken place over the course of a decade.
The complex relationship between human activities and the environment has been the focus
of extensive research and worry. As the global community grapples with swift urbanization,
population expansion, and economic progress, the effects on natural ecosystems are becoming
more evident. A crucial element of this impact is the alteration of vegetation cover, which plays a
significant role in maintaining the ecological equilibrium of our planet.Land serves as the foundation for all human activities and provides the necessary materials for
these activities. As the most crucial natural resource, its utilization by humans results in different
'Land uses,' which are determined by both human activities and the physical characteristics of the
land.
The utilization of land is impacted by human needs and environmental factors. In countries
like India, rapid population growth and the emphasis on extensive resource exploitation can lead
to significant land degradation, adversely affecting the region's land cover.
Therefore, human intervention has significantly influenced land use patterns over many
centuries, evolving its structure over time and space. In the present era, these changes have
accelerated due to factors such as agriculture and urbanization. Information regarding land use and
cover is essential for various planning and management tasks related to the Earth's surface,
providing crucial environmental data for scientific, resource management, policy purposes, and
diverse human activities.
Accurate understanding of land use and cover is imperative for the development planning
of any area. Consequently, a wide range of professionals, including earth system scientists, land
and water managers, and urban planners, are interested in obtaining data on land use and cover
changes, conversion trends, and other related patterns. The spatial dimensions of land use and
cover support policymakers and scientists in making well-informed decisions, as alterations in
these patterns indicate shifts in economic and social conditions. Monitoring such changes with the
help of Advanced technologies like Remote Sensing and Geographic Information Systems is
crucial for coordinated efforts across different administrative levels. Advanced technologies like
Remote Sensing and Geographic Information Systems
9
Changes in vegetation cover refer to variations in the distribution, composition, and overall
structure of plant communities across different temporal and spatial scales. These changes can
occur natural.
This slide is special for master students (MIBS & MIFB) in UUM. Also useful for readers who are interested in the topic of contemporary Islamic banking.
Strategies for Effective Upskilling is a presentation by Chinwendu Peace in a Your Skill Boost Masterclass organisation by the Excellence Foundation for South Sudan on 08th and 09th June 2024 from 1 PM to 3 PM on each day.
How to Add Chatter in the odoo 17 ERP ModuleCeline George
In Odoo, the chatter is like a chat tool that helps you work together on records. You can leave notes and track things, making it easier to talk with your team and partners. Inside chatter, all communication history, activity, and changes will be displayed.
বাংলাদেশের অর্থনৈতিক সমীক্ষা ২০২৪ [Bangladesh Economic Review 2024 Bangla.pdf] কম্পিউটার , ট্যাব ও স্মার্ট ফোন ভার্সন সহ সম্পূর্ণ বাংলা ই-বুক বা pdf বই " সুচিপত্র ...বুকমার্ক মেনু 🔖 ও হাইপার লিংক মেনু 📝👆 যুক্ত ..
আমাদের সবার জন্য খুব খুব গুরুত্বপূর্ণ একটি বই ..বিসিএস, ব্যাংক, ইউনিভার্সিটি ভর্তি ও যে কোন প্রতিযোগিতা মূলক পরীক্ষার জন্য এর খুব ইম্পরট্যান্ট একটি বিষয় ...তাছাড়া বাংলাদেশের সাম্প্রতিক যে কোন ডাটা বা তথ্য এই বইতে পাবেন ...
তাই একজন নাগরিক হিসাবে এই তথ্য গুলো আপনার জানা প্রয়োজন ...।
বিসিএস ও ব্যাংক এর লিখিত পরীক্ষা ...+এছাড়া মাধ্যমিক ও উচ্চমাধ্যমিকের স্টুডেন্টদের জন্য অনেক কাজে আসবে ...
This presentation was provided by Steph Pollock of The American Psychological Association’s Journals Program, and Damita Snow, of The American Society of Civil Engineers (ASCE), for the initial session of NISO's 2024 Training Series "DEIA in the Scholarly Landscape." Session One: 'Setting Expectations: a DEIA Primer,' was held June 6, 2024.
Pride Month Slides 2024 David Douglas School District
Anu thubagere breadagami_fri
1. Team Presentation for Friday, Sept 28th
Final Team Presentation for Friday, Sept 28th
Get out of the building and talk to 10-15 customers Guillaume:
· Get out of the building and talk to 10-15 customers François!Rouelle!
presentation format:
Final presentation format:
Breadagami
J.!B.!M.!Bucquet!
2 minute Youtube video
o · 2 minute Youtube video
Revolutionize Membrane Protein
examples here: http://www.slideshare.net/sblank/videos
Crystallography
o examples
Day 1: 16
20 minutes presentation here: http://www.slideshare.net/sblank/videos
o · 20 2: 10 presentation
minutes Antoine!François!
examples here: http://www.slideshare.net/sblank/tagged/Columbia
Day o examples here: http://www.slideshare.net/sblank/tagged/Columbia
o and here http://www.slideshare.net/sblank/tagged/stanford
Day 3: 11 here http://www.slideshare.net/sblank/tagged/stanford
o and
Here's what we thought - day 1
o · Here's6
Louis!Nicolas!
Day 1 4: what we thought - day 1
Day canvas 1 canvas Vauquelin!
o Day
o diagrams if you can draw any
TotaloInterviews: 42can draw any
o Text summary if not if you
diagrams
o Text summary if
Here's what we learned - days 2-4not
Friedrich!
Stromeyer!
· Here's what we learned - days 2-4
o Day 2-4 canvases
o Day 2-4 canvases
o Use diagrams
o Use diagrams
Here's where we ended - day 5 Robert!Bunsen!
· Here's where we ended - day 5
o Use diagrams
o Use diagrams
o Text summary
o Text summary
Here’s What We Are Going to Do Next Viktor!Meyer!
· Here’s What We Are Going to Do Next
Moses!Gomberg!
John!C.!Bailar,!Jr.!
R.!G.!Pearson! Fred!Basolo!
Anu Thubagere Fadl Saadi Andrew Hoff Matt Gethers
Graduate Student Graduate Student Graduate StudentHarry!Gray! Graduate Student
Bioengineering Materials Science Materials Science Bioengineering
2. What we thought on Day 1
Novel Technology: Ability to tether biomolecules such as
DNA/RNA/Proteins on a wafer
Initial thoughts:
• Target market would be Academics and
pharmaceutical companies who wished to do
in-vitro studies
• Spatial Control main selling point
3. Business Canvas V1
Oligo manufacturers. Must make chips. Make We can provide a We provide the chip and Academics and
Perhaps strike agreement “master mix” that is scaffold onto which the DNA Scaffold. The pharmaceutical
making chemistry added to oligo staples. small molecules and customer provides the companies who want to
conjugating molecule of Must maintain biologics can be oligo “staples” that are test interactions
interest to oligo standard advertising in journals templated to allow for conjugated to the between small
part of repertoire. and other venues. experiments with high molecules of interest. molecules and biologics
Perhaps they could have spatial control. with high spatial control.
a pool of oligos that are
used specifically for our
devices.
Access to E-beam Advertising in journals.
Also need an agreement lithography. Making Could market services
with facility that has master mix. Must through larger supplier
electron beam cultivate relationships like Invitrogen, Sigma.
lithography capability. with oligo companies.
Relationships with
suppliers?
Must buy chips, constituents of master mix. Need Either direct sale to customers or payment
storage facility. Need access to or need to own SEM. from suppliers when each chip is bought.
4. What we learned on Day 1
• Found three potential markets for our
technology
– Stem Cell Researchers
– Cancer Pharmaceutical Companies
– Protein Crystallography
• No silver bullet yet, many suggestions are for
fields outside of experts’ own
5. Business Canvas V2
Oligo manufacturers. Perhaps strike Must make chips. Make “master We can provide a scaffold onto We provide the chip and the DNA Academics and pharmaceutical
agreement making chemistry mix” that is added to oligo staples. which small molecules and biologics Scaffold. The customer provides the companies who want to test
conjugating molecule of interest to Must maintain advertising in can be templated to allow for oligo “staples” that are conjugated to interactions between small
oligo standard part of repertoire. journals and other venues. experiments with high spatial the molecules of interest. molecules and biologics with high
Perhaps they could have a pool of control. spatial control.
oligos that are used specifically for our Product development Personal Assistance
devices.
Eliminate uncertainty in
Also need an agreement with facility Shipping quantity of stimulants Automated Service Stem cell researchers
that has electron beam lithography
capability. Customer Service Simplify control of Word of mouth
Crystallographers
molecular orientation
Kavli Nanoscience Cancer pharmaceutical
Institute (Caltech) Advertising in journals. Could market companies
Access to E-beam lithography. services through larger supplier like
Making master mix. Must cultivate
Invitrogen, Sigma.
Silicon Wafer Supplier relationships with oligo companies.
Relationships with suppliers?
Integrated DNA Trade shows,
Technologies Technical expertise Conferences,
Publications, Internet
Glen Research Fabrication Facilities
Shipping
Must buy chips, constituents of master mix. Need Yearly subscription model
storage facility. Kavli Nanoscience Institute partnership.
Direct sales
6. What we learned on Day 2
• Interviewed 4 Protein Crystallographers
– 3 of them said they were ‘not interested in our product at
all’
– 1 said it was very interesting for Membrane Protein
Crystallography
• One third of all proteins
• Very difficult to obtain crystal structure
• Started winnowing interesting fields and focusing on
those where technology could have maximum impact
– Removed cancer research
– Ceased searching for new fields
• Started resorting to cold calls
7. Business Canvas V3
Kavli Nanoscience Product development Eliminate uncertainty in Cancer pharmaceutical
Institute (Caltech) quantity of stimulants companies
Shipping Automated Service
Silicon Wafer Supplier Simplify control of
Customer Service molecular orientation Word of mouth Stem cell researchers
Integrated DNA
Differentiate cells with Membrane Protein
Technologies
much higher yield and Crystallographers
Glen Research greater reliability
VWR International Make crystallization
Direct Sales Force
faster & simpler
IP
Trade shows,
Technical expertise Conferences,
Publications, Internet
Fabrication Facilities
Vendor
Must buy chips, constituents of master mix. Need Yearly subscription model
storage facility. Kavli Nanoscience Institute partnership.
Direct sales
8. What we Learned on Day 3
• Membrane Protein Crystallographers are good
early adopters
– Many have resorted to in house technology to
resolve situation
– Focus of Membrane Protein Crystallography
• Specialized market limited need for
Vendors
• Trade shows and conferences are extremely
important in this field
9. Business Canvas V4
Kavli Nanoscience Get: Word of mouth &
Product development Make crystallization Stem cell researchers
Institute (Caltech) Publications &
faster & simpler
Conferences
Shipping
Silicon Wafer Supplier Membrane Protein
Problem: Crystallizing Keep: Customer Service
Customer Service Crystallographers
Membrane Proteins
Integrated DNA “It’s voodoo” Grow: Cross Selling
Technologies Best case: couple
months
Glen Research Often: Never
TEM Manufacturers
Solution: Direct Sales Force
VWR International IP -Easy to use kit
-Works for all Trade shows
Technical expertise -One tenth of the time
required Vendor
Fabrication Facilities
SBIR/STTR
Must buy chips, constituents of master mix. Need Yearly subscription model
storage facility. Kavli Nanoscience Institute partnership.
Direct sales
10. What we learned on Day 4
• Numbers
– Time scale
– Cost
– Yield
“[If you can do this], it would be revolutionary…the world
would be at your door”
James Bowie
Professor and Vice-Chair; Department of Chemistry and Biochemistry, UCLA-
DOE Institute for Genomics and Proteomics and Molecular Biology Institute
11. Current Business Canvas
Kavli Nanoscience Problem: Crystallizing Get: Word of mouth
Product development Publications Membrane Protein
Institute (Caltech) Membrane Proteins
Conferences Crystallographers
Shipping “It’s voodoo”
Silicon Wafer Supplier Best case: couple
Customer Service Keep: Customer Service -Grad Student
months
-Postdoctoral Scholar
Integrated DNA Often: Never
Grow: Cross Selling -Staff Scientist
Technologies
Solution:
Glen Research -Easy to use kit
-Works consistently
-One tenth of the time
required Direct Sales Force
IP
Trade shows
Technical expertise
Fabrication Facilities
SBIR/STTR/Angel
Consumables (wafers, oligos etc.) Personnel Direct sales
Storage and Work Facility Conference Travel
Kavli Nanoscience Institute partnership
12. Business Canvas V1
Oligo manufacturers. Must make chips. Make We can provide a We provide the chip and Academics and
Perhaps strike agreement “master mix” that is scaffold onto which the DNA Scaffold. The pharmaceutical
making chemistry added to oligo staples. small molecules and customer provides the companies who want to
conjugating molecule of Must maintain biologics can be oligo “staples” that are test interactions
interest to oligo standard advertising in journals templated to allow for conjugated to the between small
part of repertoire. and other venues. experiments with high molecules of interest. molecules and biologics
Perhaps they could have spatial control. with high spatial control.
a pool of oligos that are
used specifically for our
devices.
Access to E-beam Advertising in journals.
Also need an agreement lithography. Making Could market services
with facility that has master mix. Must through larger supplier
electron beam cultivate relationships like Invitrogen, Sigma.
lithography capability. with oligo companies.
Relationships with
suppliers?
Must buy chips, constituents of master mix. Need Either direct sale to customers or payment
storage facility. Need access to or need to own SEM. from suppliers when each chip is bought.
13. Workflow for creating Membrane
Protein Crystals
Cloning
Heterologous expression
Screening for detergents
Membrane preparation
Solubilization
IMAC purification
Gel filtration
Protein characterization
Ion exchange
Crystal screening
Crystal optimization Our Target
X-ray diffraction
Newby, Z, Nature Protocols 4, 619 (2009)
14. Day in the Life Diagram
Before
2 days 1-3 Months 10%
Micelle + Protein + Detergent + 10 Plate Structural
Crystal Analyze Structure
Robot Information
National Lab
Pass
Fail
90%
Total Cost: $200,000
After
2 days Days 90%
Analyze Structural
Micelle + Protein + Detergent + 10 Breadagami Crystal
Robot Structure Information
Local
Pass
equipment
Fail
Total Cost: $100,000
10%
15. Archetype/Ecosystem
Journal • Influencer Funding • Influencer Department • Influencer
Reviewer • Saboteur Agencies •Economic Head
Decider
• Influencer
PI • Economic Decider
• Possible Saboteur
• User • User
• More discretionary Staff • More discretionary
Post-Doc funds Scientist funds
• Early adopter • Early adopter
• User
Grad
• Some discretionary
Students
funds
and Techs
• Early adopter
Reagent/Equi
pment • Provider
Suppliers
16. Minimum Viable Product
Buffers
Patterned chip
Other Vendors 2-D protein crystals
Breadagami
• Easy to use
• Works with off the shelf consumables
• One size fits all
17. Value Proposition
Value proposition:
• Remove uncertainty
• Order of magnitude decrease of time spent to obtain structural
information of membrane proteins
• Higher research throughput
• Less consumables
Value added to customer:
• Old Process: Cost of obtaining structural information of a membrane
protein: $200,000 “Likely an underestimate”*
• New Process: Target cost of obtaining structural information of a
membrane protein: ~$100,000
– Graduate student cost: 9 months $37,500
– Consumables: $12,500
– Breadagami: $50,000 (@$5,000/chip)
*Prof. James Bowie
18. Addressable Market
Total Addressable Market (TAM):
~300 researchers worldwide
Served Addressable Market (SAM):
~150 researchers in USA
Current Target Market:
75% of SAM @ $60k/year/researcher
Estimated sales of ~$7 million/year
Future trends:
• Large increase in number of researchers & funds
• New customer segments develop (pharma)
19. Customer Relationships
Get:
Outreach with Trade Shows Conferences
Workshops
Partnering with prominent researchers
Keep:
Automated, simple system for reordering
Email updates on new uses of technology
Grow:
Cross selling products (buffers, etc.)
20. Revenue Model
• Revenue stream: Direct Sales,
• Pricing: Fixed, value, volume pricing, $5000/chip
•~10 chips for 1 crystal ie $50,000
•Possible cut off limit for Grad Students & Tech
Grad Student/Tech
Breadagami
Principal Investigator
Post-Doc/Senior scientists
• Revenue Streams: Direct Sales
• Pricing: Fixed, value, volume
21. Next Steps
• Talk to more Membrane Protein
Crystallographers to refine value proposition
• Create prototype
• Patent
• Publish
22. What we Really Learned
• You don’t know everything from Day 1
• Go and talk to potential customers
• Don’t be discouraged when you get bad news
• Situation is always more complex than
previously thought
• Being an Entrepreneur is tough!
23. Acknowledgments
• Interviewees
• Classmates
• Charles House
• Andy Sack
• Ken Pickar
• Jon Feiber
• Steve Blank
24. Channel
Vendor
Local (University) Marketplace
Breadagami
Customer
• Typical vendors would be Sigma, VWR,
Invitrogen
• Eager early adopters
• Small community
25. Revenue Model
Year 1 (of sales) Year 2
• Cost: • Cost:
– Raw material ~$300/wafer – Raw material ~$300/wafer
– Lab space ~$13,000/year (1 – Lab space ~$26,000/year (2 person
person space) space)
– Kavli Nanoscience Institute – Kavli Nanoscience Institute
~$30,000/year ~$60,000/year
– Cost of personnel – Cost of living ~$200,000/year
~$50,000/year
• Goal: sell 1000 kits in year 2
• Goal: sell 100 kits in year 1 @ $5000/kit
@ $5000/kit
– Revenue: $5 million
– Revenue: $50,000
– Cost: $586,000
– Cost: $80,000
– Profit: $4.4 million
– Profit: -$30,000