3. Who are we?
Bioconjugation
What is nitzipper®
?
How to use nitzipper®
?
Why to use nitzipper®
?
Applications
Because your goal is the priority
4. Who are we?
Here at nanoimmunotech, a European company in the Nanobiotechnology sector,
we are specialized and considered a benchmark in Bioconjugation.
The company has highly qualified and internationally recognized human resources,
state-of-the-art laboratory capabilities, standardized and proprietary protocols
and finally, the know-how to perform proper supervision, advising and validation
while creating different nanosystems or biomaterials; first step to their previous use
in biotechnological applications for different sectors (biomedical, pharmaceutics,
cosmetics, veterinary, agro-food, environmental, etc.).
At nanoimmunotech, we believe in creative researchers who want to explore new
territories. We believe in those who do not like to be told how to do something nor
be restricted or limited in capabilities. We believe in those whose goal is not just
having tools but needing those tools to achieve their goals.
This is why, for all of you independent researchers with scientific curiosities, we
have created an innovative technology called nitzipper®. The simplest, quickest
and most versatile bioconjugation technology that makes it possible for you to
join many of the molecules, biomolecules, particles, linkers, drugs, etc.You need
to create the most inspiring materials or applications.
because your goal is the priority, not the tool you use to achieve it.
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Because your goal is the priority, not the tool you use to achieve it.
5. Bioconjugation
Nowadays, the main problem regarding bioconjugation is that the conjugation
processes are different, depending on the variety of micro/nanostructures or
biomolecules used; mainly due to the wide gap existing between them in terms
of size, surface area, density of reactive groups, shape, colloidal stability, etc.
Moreover, when designing a material bioconjugation protocol, it is important to
respect the orientation of biomolecules on the surface of the material in order to
maintain its biological activity, and therefore its efficiency.
Currently, there is no universal tool that would easily enable conjugation of similar
particles or bio/molecules with one another while ensuring oriented unions,
much less the ability to easily conjugate different kinds of molecules with each
other. Consequently, customers require tailor-made projects in order to optimize
complex binding protocols. With experienced personnel, projects are time
consuming and expensive solutions completed through inefficient bioconjugation
methods. The other option is not any better: customers need to purchase specific,
individual kits for the conjugation of specific molecules and particles, for their
use in specific applications, such as magnetic separation, antibody labeling, etc.,
without ensuring obtaining good results.
With nitzipper®
, the new innovative technology of nanoimmunotech, we offer you
the almost universal, economical and single-step bioconjugation solution which
allows mono- and multi-conjugation of most micro/nanostructures, biomolecules,
linkers, drugs and other molecules, creating powerful micro/nanosystems, resulting
in the sum of the functions for each of its components.
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6. What is nitzipper®
?
nitzipper®
is a new and revolutionary family of products ready to be used in an
easy, immediate and effective conjugation through an innovative bioconjugation
technology based on the complementarity of the Linker U and Linker T, that allows
you to combine many different types of micro/nanostructures, biomolecules
(antibodies, dyes, enzymes, peptides and other molecules), drugs, etc., in
practically any combination, in an oriented way and without modifying their
properties.
NITZIPPER®
added value
Its great VERSATILITY that opens infinite doors to the bioconjugation world.
With nitzipper®
you can also perform multi-functionalization, joining not only
two different types of molecules or micro/nanostructures with each other, but
many of them simultaneously, making possible to create a multitude of new and
sophisticated conjugates for their use in infinite applications.
It is not a specific solution for a specific application. The nitzipper®
products
can be used to improve traditional applications (antibody labeling, immunoassays,
flow cytometry, ELISA, biosensing, magnetic separation, and many others) but
also to explore even the most unthinkable new applications.
You already have developed your own biomolecule or micro/nanostructure and you want to conju-
gate it? We can make it compatible with the nitzipper®
technology and so, you will be able to use
it with any other nitzipper®
product.
Versatility, regarding all the products combinations you can design
A strong and stable
union between the
different pieces of
the micro/nanosys-
tems is performed
thanks to matching
linkersnamedLinker
U and Linker T, each
one on the surface
of nitzipper®
pieces.
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7. Once you have received the nitzipper®
products, simply mix the appropriate
quantity of piece with Linker U you wish to
connect with the piece with Linker T and wait
a few minutes to quickly achieve a stable
conjugation between both pieces.
nitzipper® is compatible with many more structures and
moleculesthantheonesyouseeinthestore. Ifyoucannotfind
the pieces you need or if you want us to make a custom piece,
specific for your micro/nanostructures and/or molecules,
compatible with our technology, just contact us:
customer_support@nanoimmunotech.es
How to use nitzipper®
? A SINGLE BIOCONJUGATION STEP
You now have your conjugate
ready to use!
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Select and purchase the products/pieces that you wish to conjugate
in our nitzipper® store. As an example, you can choose to purchase
a magnetic nanoparticle with Linker U (yellow tape tube) and an
antibody with Linker T (red tape tube), or an antibody with Linker U
and a dye with Linker T or… The possibilities are endless.
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8. Let’s focus on what really matters. Whether you have the
knowledge on conjugation techniques or not, in just 20-
30 seconds you will have performed all the manipulations
required to conjugate different compounds.
Saves Time
Simplicity
Why to use nitzipper®
?
Versatility
Avoids searching for different solutions for every
type of conjugation and helps reduce the stock of
products you need.You can bioconjugate any kind of
nitzipper®
piece with any other. You simply need to choose
pieces with the matching Linker U and Linker T.
Biomolecules are conjugated in a properly oriented direction
avoiding, for example, blocking the antibody recognition
region. As a result, you receive better results for your
application.
Better Results
nitzipper®
is a strong and stable union that binds in an
oriented way and without altering the properties of the
micro/nanostructures or molecules.
Stable
Strong and stable bonds. nitzipper®
can be used in many
applications with the certainty that it will work.
Efficiency
High-standard results can be obtained with less quantity of
biomolecules or particles, which significantly reduces your
conjugation costs.
Reduce costs
=
+ 11
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With a single step of mixing the pieces of Linker U with the
pieces of Linker T, you will be able to carry out the
bioconjugation of your micro/nanostructures and/or
molecules, without being an expert or needing any additional
equipment.
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9. “nitzipper®
is the simplest, quickest
and most versatile bioconjugation
solution
”
Efficiency Medium Medium Medium
Parameters nitzipper® Biotinylation kit Other Bioconjugation kits (propietary linker technology)Amine reactive linker
Operator’s Manipulation Time
Preactivation step
Bioconjugation Overall Time
Desalting and/or purification step
Application Versatility
Operator’s Level of Qualification
Average Cost
Efficiency
Incompatibilities
Technologies
High Medium Medium
1h to 24h
30min 30min
30min to 3h
20-30sec up to 1h
30min to 24h
required required coul be required
15min
20-30sec
not required
not required
not required could be required could be required
Medium
High
No
Medium
Medium
Medium
may vary
Low Medium
Medium
Medium
Amine
cointaining
buffers
Background
in application
due to endogen
biotin
Medium-High
Medium Medium
Medium
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10. Biosensing
Versatility that opens new doors to
infinite applications
Flow cytometryMagnetic Separation
Nanovaccines
Immuno-PCR
Antibody Labelling
Immunofluorescence
Immunoassays
ELISA
Magnetic hyperthermia
Develop your own application with nitzipper bioconjugation technology
Applications
Thousands of scientists, both in academia and industry, use bioconjugation in
multiple applications.
Thanks to its incredible versatility, nitzipper®
bioconjugation technology offers
an endless panel of applications, performing a variety of different combinations
with varied micro/nanostructures and/or biomolecules within different
applications. Additionally, each combination can be utilized for different kinds of
applications.
Here you can find some examples of applications that you can develop with
nitzipper®
. Some of them were performed by nanoimmunotech while others
are results our customers achieved through different uses and applications in
many different sectors such as: biotechnology, pharmaceutics, cosmetics, agro
food, environment, veterinary and many others. With or without experience in
bioconjugation, our customers achieve successful results, thanks to nitzipper®
technology.
So focus on your own application and be the next to use nitzipper®
. Remember,
nitzipper®
applications are infinite, only limited by your own imagination.
Should you need assistance or advice when planning or designing your new
application, just let us know and we will be glad to help you:
customer_support@nanoimmunotech.es
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11. HEADQUARTERS IN ZARAGOZA, SPAIN
Edificio Cero Emisiones
Avda. Autonomía 7
0003 Zaragoza, Spain
Phone: (+34) 976 369 300
HEADQUARTERS IN VIGO, SPAIN
Edificio CITEXVI
Fonte das Abelleiras s/n
Campus Universitario de Vigo
36310 Vigo, Pontevedra, Spain
Phone: (+34) 986 167 990
info@nanoimmunotech.es
www.nitzipper.com
Your Bioconjugation Friendly Solution
