Microscopy - Magnification, Resolving power, Principles, Types and ApplicationsNethravathi Siri
Magnification, Resolving power, Principles and Applications of Simple, Compound, Stereozoom, Phase contrast, Fluorescent and Electron microscopes (TEM & SEM).
Microscopy is the technical field that uses microscopes to observe samples which are not in the resolution range of the normal-unaided eye.
The Microscope Zacharias Janssen is to be credited for i.docxoreo10
The Microscope
Zacharias Janssen is to be credited for inventing the first truly compound microscope
Microscope
What is a microscope?It is a tool or instrument often used in laboratories to aid in viewing organisms that cannot be seen with an unaided eye
Types of microscope
Biologist often used different microscope
Compound light microscope
Dissection microscope
Scanning electron microscope(SEM)
Transmission electron microscope(TEM)
but of all, the compound light microscope is what we shall be using
Dissecting Vs. Compound light microscope
Scanning Vs. Transmission electron Microscope
Microscope Compound light microscope is simply a microscope that has at least two magnifying lenses (the oculars and the objectives)Binocular microscope possess two eyepieces (what we shall be using in the lab)Monocular microscope possess one eyepieces
Bi = two mono = one
Microscopes Electron microscope magnifies the specimen 1000X larger than the light microscopeThe Dissecting microscope on the other hand has a low magnification power (7X to 30X). It is used to view large objects
*
Microscope There are a variety of light microscope but each differs by the emission of light through the specimen that is viewed
Phase contrast
Darkfield
Polarizing
UV
Terms Bright Field: illumination, sample contrast comes from absorbance of light in the sample.
Cross polarized light: illumination, sample contrast comes from rotation of polarized light through the sample.
TermsDark Field: illumination, sample contrast comes from light scattered by the sample.Working distance: distance between the specimen and the objective lens ( is this distance greater with the 40x or the 10x objective ? Ans: 10x )
Phase Contrast: illumination, sample contrast comes from interference of different path lengths of light through the sample.
labelsLight source, a light or a mirror (7)Diaphragm and condenser lens (8)Objective (3)Ocular lens (eyepiece) (1)In addition the vast majority of microscopes have the same 'structural' components:Objective turret/ nosepiece (to hold multiple objective lenses) (2)
LabelsStage (to hold the sample) (6)Focus wheel to move the stage (4 - coarse adjustment, 5 - fine adjustment) Stage adjustment knobs (9)
Terms used when dealing with a microscope
The headIt supports the two sets of magnifying lenses
The Oculars (eyes)It is the lenses used to view the specimen.Located on the eyepieceHas a magnification of 10X
Microscope
Interpupillary distanceThis is the distance between the eyepiece on a binocular microscopeIt can be adjusted to suit your eyes
Field of view “FOV”the diameter of the circle of light that you see when looking into a microscope
Microscope (cont..)
ObjectivesThese are the four lenses on the revolving nosepieceFour types exist: 4x , 10x , 40x , 100x
4X : the scanning lens
10X: the intermediate lens
40X: the high power lens
100X: the oil immersion lens
Microscope(cont..)
The ArmThe part ...
Types of Microscopes with their applications - Microbiologynote.com
https://microbiologynote.com/types-of-microscopes-with-their-applications/
Youtube Lecture Video:
https://www.youtube.com/watch?v=nuJZtXohFFQ&ab_channel=MicrobiologyNote
How to Create Map Views in the Odoo 17 ERPCeline George
The map views are useful for providing a geographical representation of data. They allow users to visualize and analyze the data in a more intuitive manner.
Microscopy - Magnification, Resolving power, Principles, Types and ApplicationsNethravathi Siri
Magnification, Resolving power, Principles and Applications of Simple, Compound, Stereozoom, Phase contrast, Fluorescent and Electron microscopes (TEM & SEM).
Microscopy is the technical field that uses microscopes to observe samples which are not in the resolution range of the normal-unaided eye.
The Microscope Zacharias Janssen is to be credited for i.docxoreo10
The Microscope
Zacharias Janssen is to be credited for inventing the first truly compound microscope
Microscope
What is a microscope?It is a tool or instrument often used in laboratories to aid in viewing organisms that cannot be seen with an unaided eye
Types of microscope
Biologist often used different microscope
Compound light microscope
Dissection microscope
Scanning electron microscope(SEM)
Transmission electron microscope(TEM)
but of all, the compound light microscope is what we shall be using
Dissecting Vs. Compound light microscope
Scanning Vs. Transmission electron Microscope
Microscope Compound light microscope is simply a microscope that has at least two magnifying lenses (the oculars and the objectives)Binocular microscope possess two eyepieces (what we shall be using in the lab)Monocular microscope possess one eyepieces
Bi = two mono = one
Microscopes Electron microscope magnifies the specimen 1000X larger than the light microscopeThe Dissecting microscope on the other hand has a low magnification power (7X to 30X). It is used to view large objects
*
Microscope There are a variety of light microscope but each differs by the emission of light through the specimen that is viewed
Phase contrast
Darkfield
Polarizing
UV
Terms Bright Field: illumination, sample contrast comes from absorbance of light in the sample.
Cross polarized light: illumination, sample contrast comes from rotation of polarized light through the sample.
TermsDark Field: illumination, sample contrast comes from light scattered by the sample.Working distance: distance between the specimen and the objective lens ( is this distance greater with the 40x or the 10x objective ? Ans: 10x )
Phase Contrast: illumination, sample contrast comes from interference of different path lengths of light through the sample.
labelsLight source, a light or a mirror (7)Diaphragm and condenser lens (8)Objective (3)Ocular lens (eyepiece) (1)In addition the vast majority of microscopes have the same 'structural' components:Objective turret/ nosepiece (to hold multiple objective lenses) (2)
LabelsStage (to hold the sample) (6)Focus wheel to move the stage (4 - coarse adjustment, 5 - fine adjustment) Stage adjustment knobs (9)
Terms used when dealing with a microscope
The headIt supports the two sets of magnifying lenses
The Oculars (eyes)It is the lenses used to view the specimen.Located on the eyepieceHas a magnification of 10X
Microscope
Interpupillary distanceThis is the distance between the eyepiece on a binocular microscopeIt can be adjusted to suit your eyes
Field of view “FOV”the diameter of the circle of light that you see when looking into a microscope
Microscope (cont..)
ObjectivesThese are the four lenses on the revolving nosepieceFour types exist: 4x , 10x , 40x , 100x
4X : the scanning lens
10X: the intermediate lens
40X: the high power lens
100X: the oil immersion lens
Microscope(cont..)
The ArmThe part ...
Types of Microscopes with their applications - Microbiologynote.com
https://microbiologynote.com/types-of-microscopes-with-their-applications/
Youtube Lecture Video:
https://www.youtube.com/watch?v=nuJZtXohFFQ&ab_channel=MicrobiologyNote
How to Create Map Views in the Odoo 17 ERPCeline George
The map views are useful for providing a geographical representation of data. They allow users to visualize and analyze the data in a more intuitive manner.
The Roman Empire A Historical Colossus.pdfkaushalkr1407
The Roman Empire, a vast and enduring power, stands as one of history's most remarkable civilizations, leaving an indelible imprint on the world. It emerged from the Roman Republic, transitioning into an imperial powerhouse under the leadership of Augustus Caesar in 27 BCE. This transformation marked the beginning of an era defined by unprecedented territorial expansion, architectural marvels, and profound cultural influence.
The empire's roots lie in the city of Rome, founded, according to legend, by Romulus in 753 BCE. Over centuries, Rome evolved from a small settlement to a formidable republic, characterized by a complex political system with elected officials and checks on power. However, internal strife, class conflicts, and military ambitions paved the way for the end of the Republic. Julius Caesar’s dictatorship and subsequent assassination in 44 BCE created a power vacuum, leading to a civil war. Octavian, later Augustus, emerged victorious, heralding the Roman Empire’s birth.
Under Augustus, the empire experienced the Pax Romana, a 200-year period of relative peace and stability. Augustus reformed the military, established efficient administrative systems, and initiated grand construction projects. The empire's borders expanded, encompassing territories from Britain to Egypt and from Spain to the Euphrates. Roman legions, renowned for their discipline and engineering prowess, secured and maintained these vast territories, building roads, fortifications, and cities that facilitated control and integration.
The Roman Empire’s society was hierarchical, with a rigid class system. At the top were the patricians, wealthy elites who held significant political power. Below them were the plebeians, free citizens with limited political influence, and the vast numbers of slaves who formed the backbone of the economy. The family unit was central, governed by the paterfamilias, the male head who held absolute authority.
Culturally, the Romans were eclectic, absorbing and adapting elements from the civilizations they encountered, particularly the Greeks. Roman art, literature, and philosophy reflected this synthesis, creating a rich cultural tapestry. Latin, the Roman language, became the lingua franca of the Western world, influencing numerous modern languages.
Roman architecture and engineering achievements were monumental. They perfected the arch, vault, and dome, constructing enduring structures like the Colosseum, Pantheon, and aqueducts. These engineering marvels not only showcased Roman ingenuity but also served practical purposes, from public entertainment to water supply.
Operation “Blue Star” is the only event in the history of Independent India where the state went into war with its own people. Even after about 40 years it is not clear if it was culmination of states anger over people of the region, a political game of power or start of dictatorial chapter in the democratic setup.
The people of Punjab felt alienated from main stream due to denial of their just demands during a long democratic struggle since independence. As it happen all over the word, it led to militant struggle with great loss of lives of military, police and civilian personnel. Killing of Indira Gandhi and massacre of innocent Sikhs in Delhi and other India cities was also associated with this movement.
How to Make a Field invisible in Odoo 17Celine George
It is possible to hide or invisible some fields in odoo. Commonly using “invisible” attribute in the field definition to invisible the fields. This slide will show how to make a field invisible in odoo 17.
2024.06.01 Introducing a competency framework for languag learning materials ...Sandy Millin
http://sandymillin.wordpress.com/iateflwebinar2024
Published classroom materials form the basis of syllabuses, drive teacher professional development, and have a potentially huge influence on learners, teachers and education systems. All teachers also create their own materials, whether a few sentences on a blackboard, a highly-structured fully-realised online course, or anything in between. Despite this, the knowledge and skills needed to create effective language learning materials are rarely part of teacher training, and are mostly learnt by trial and error.
Knowledge and skills frameworks, generally called competency frameworks, for ELT teachers, trainers and managers have existed for a few years now. However, until I created one for my MA dissertation, there wasn’t one drawing together what we need to know and do to be able to effectively produce language learning materials.
This webinar will introduce you to my framework, highlighting the key competencies I identified from my research. It will also show how anybody involved in language teaching (any language, not just English!), teacher training, managing schools or developing language learning materials can benefit from using the framework.
The Art Pastor's Guide to Sabbath | Steve ThomasonSteve Thomason
What is the purpose of the Sabbath Law in the Torah. It is interesting to compare how the context of the law shifts from Exodus to Deuteronomy. Who gets to rest, and why?
Model Attribute Check Company Auto PropertyCeline George
In Odoo, the multi-company feature allows you to manage multiple companies within a single Odoo database instance. Each company can have its own configurations while still sharing common resources such as products, customers, and suppliers.
Unit 8 - Information and Communication Technology (Paper I).pdfThiyagu K
This slides describes the basic concepts of ICT, basics of Email, Emerging Technology and Digital Initiatives in Education. This presentations aligns with the UGC Paper I syllabus.
8. Keep in mind...
The Occular lens
= 10X
(sometimes15X)
The Scanning objective lens
=4X
The Low Power
objective lens =
10X
The High Power objective
lens = 40X
9. What is the total magnification when the High
power objective is in place?
200X 400X
100X 40X
15. Expectations – Unlimited attempts on Homework Assignments
This week, you have 5 assignments to complete:
□ Lesson #2 – Regional Terms Assignment (Visible Body) Due Friday
□ Lesson #2 – Cytology Assignment (Visible Body) Due Friday
□ lesson #2- Regional Terms Online Homework Assignment (Blackboard) Due Sunday
Lesson #2- Microscopy and Cytology Online Homework Assignment (Blackboard) Due Sunday
Lab Goup Wiki Project- Part 1
To help you prepare for these assignments, you should do the following:
1. Complete all parts of this worksheet. Use Online Guided lesson. Found in Google Drive and VB
2. Practice identifying the parts of the body using specific anatomical regional terms.
3. Practice naming the parts of the microscope & describing their functions.
4. Practice calculating total magnification.
5. Practice explaining the major principles of microscopy.
6. Practice identifying the parts of the cell on models & describing the functions of each.
7. VISIT THE SCIENCE LEARNING CENTER (SLC) Virtual Tutoring Page!*
*REMEMBER: The Science Learning Center has a group of tutors trained to assist you with your A&P class.
