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Introduction-to-Microscopy.pptx
1. Earl Adam B. Prado, RMT, MLS (ASCPi)
Introduction to Microscopy
Earl Adam B. Prado, RMT, MLS (ASCPi)
2. • Define microscope;
• Describe the tenets of microscope;
• Determine the key components of light microscopy;
• Identify the parts of microscope;
• To know how to handle and take care of a microscope.
Learning Objectives:
3. Microscope
• A tool used to view tissues and
organs’ microscopic structure.
• “mikros” – small
• “skopein” – to look
• Uses two lenses to magnify the
objects.
4. Tenets of Light Microscopy
• Light object (specimen) lenses final magnified virtual image
5. Key components to light microscopy
• MAGNIFICATION
1. Ocular lens – magnifies 10x
2. Objective lenses
*scanner (4x), LPO (10x), HPO(40x), OIO (100x)
Scanner
Low power
High power
Oil Immersion
6. • RESOLUTION (resolving power)
- Is the ability of the microscope to which detail in magnified is
maintained.
- The higher the magnification, the more blur the objects are.
- Optimization of resolving power:
1. Proper use of condenser and iris diaphragm.
2. Use of immersion oil.
3. Proper use of diopter and adjustment knobs.
Key components to light microscopy
7. • CONTRAST
- Needed to make objects stand out
from the background.
- Dark objects against a bright
background.
- To increase contrast = staining
techniques (e.g. H&E stain)
Key components to
light microscopy
9. Parts of a microscope
1. Base – holds various parts of the microscope
2. Arm – connects ocular and objective lenses
3. Mechanical stage – holds the microscope slide
4. Head – holds the ocular lens
5. Ocular lens
6. Objective lenses
7. Condenser – focuses a cone of light on the slide
8. Iris Diaphragm – control the light that passes through
the condenser
9. Light source
10. Fine and coarse adjustment knobs – sharpen the image
Illuminating
parts
Magnifying
parts
Mechanical
parts
10. General Procedures
• Make sure all backpacks and materials
are out of the aisles and off the tops of
desks.
desks.
• Store with cord wrapped around
microscope and the scanning objective
clicked into place.
• Carry by the base and arm with both
hands.
11. Focusing Specimens
1. Always start with the scanning objective.
Use the Coarse Knob to focus and then the fine
adjustment knob until clear, image may be small at this
magnification.
2. Once you've focused on Scanning, switch to
Low Power.
Use the Coarse Knob to refocus, then fine
adjustment until clear.
Now switch to High Power.. At this point, ONLY
use the Fine Adjustment Knob to focus specimens.
12. Recap
1. Scanning --> use coarse and fine knob
2. Low power --> use coarse and fine knob
3. High power --> use fine knob only
DO NOT
SKIP
STEPS!!!!
What will happen if I use the course knob
under high power?
The slide or lens could crack
