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Experimental Pathology Laboratories, Inc.
Stereology
Stereology Basics
Stereology is the science in which estimates of
three-dimensional measurements are obtained
using two-dimensional sampling.
There are several ways in which stereology differs from two-
dimensional image analysis:
– Stereological procedures may utilize glass slides
directly or digitized images
– Stereology provides a more accurate assessment of
the number of objects within a given three-
dimensional region of interest
– Special attention is given to procedures for
minimizing bias during specimen sampling and data
collection
– Stereology can be used to obtain measurements that
cannot be assessed using two dimensional image
analysis (e.g., the degree of blood vessel branching)
x
y
Two Dimensional Analysis vs. Stereology
2-D Image Analysis
– Counts
– Lengths
– Areas
– Color Intensity
Stereology
– Counts
– Lengths
– Surface areas
– Volumes
– Connectivity
When to Utilize Stereology
Many types of morphologic measurements are
amenable to stereological evaluation. Various examples
include:
– Estimates of neuronal cell counts within specified
brain nuclei
– Differential counts and volumes of ovarian
follicles
– Estimates of cell number, total volume, mean
volume and mean height of enterochromaffin
cells in the stomach
– Estimates of thyroid follicular colloid volumes
– Estimates of proximal tubule cell numbers and
tubular cell volume in the kidney
– Estimates of alveolar volumes in the lung
EPL Stereology Experience
• EPL has experience with modern design-
based stereological techniques
• For the past five years, EPL has worked with a
mouse model of Parkinson’s disease
• Projects utilize MBF’s Stereo Investigator
software
• Projects supervised by a toxicologic
pathologist, and performed in accordance with
GLP principles

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EPL, Inc. - Stereology Services

  • 2. Stereology Basics Stereology is the science in which estimates of three-dimensional measurements are obtained using two-dimensional sampling. There are several ways in which stereology differs from two- dimensional image analysis: – Stereological procedures may utilize glass slides directly or digitized images – Stereology provides a more accurate assessment of the number of objects within a given three- dimensional region of interest – Special attention is given to procedures for minimizing bias during specimen sampling and data collection – Stereology can be used to obtain measurements that cannot be assessed using two dimensional image analysis (e.g., the degree of blood vessel branching) x y
  • 3. Two Dimensional Analysis vs. Stereology 2-D Image Analysis – Counts – Lengths – Areas – Color Intensity Stereology – Counts – Lengths – Surface areas – Volumes – Connectivity
  • 4. When to Utilize Stereology Many types of morphologic measurements are amenable to stereological evaluation. Various examples include: – Estimates of neuronal cell counts within specified brain nuclei – Differential counts and volumes of ovarian follicles – Estimates of cell number, total volume, mean volume and mean height of enterochromaffin cells in the stomach – Estimates of thyroid follicular colloid volumes – Estimates of proximal tubule cell numbers and tubular cell volume in the kidney – Estimates of alveolar volumes in the lung
  • 5. EPL Stereology Experience • EPL has experience with modern design- based stereological techniques • For the past five years, EPL has worked with a mouse model of Parkinson’s disease • Projects utilize MBF’s Stereo Investigator software • Projects supervised by a toxicologic pathologist, and performed in accordance with GLP principles