Laminin 5: roles & utility in wound healing
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Laminin 5-containing topical medications may help to increase the speed of wound healing
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Laminin 5: roles & utility in wound healing
- 1. LAMININ 5: ROLES & UTILITY IN WOUND HEALING Peter Nugraha Soekmadji Faculty of Medicine Manaratha Christian University Immanuel Hospital Bandung
- 2. Conflict of Interest Statement • This presentation is supported by Pro-Health International
- 3. Laminins: critical structural components of basement membranes
- 9. Wound: expression of specific laminins ↑ → deposited at wound margins → substrate for reepithelialization & blood vessel growth
- 14. Laminins interact with receptors on epithelial & endothelial cells → determine behavioral responses
- 18. Laminin-derived peptides: mimic aspects of laminin functions
- 22. Laminins: critical structural components of basement membranes Wound: expression of specific laminins ↑ → deposited at wound margins → substrate for reepithelialization & blood vessel growth
- 23. Laminins interact with receptors on epithelial & endothelial cells → determine behavioral responses Laminin-derived peptides: mimic aspects of laminin functions
- 24. Future directions: scaffolding mechanisms → stable delivery of laminin-derived peptides to wound environments
- 25. references Aumailley M, Rousselle P. Laminins of the dermo-epidermal junction. Matrix Biol. 1999 Feb;18(1):19-28. Frank DE, Carter WG. Laminin 5 deposition regulates keratinocyte polarization and persist ent migration. J Cell Sci. 2004 Mar 15;117(Pt 8):1351-63. Iorio V, Troughton LD, Hamill KJ. Laminins: Roles and Utility in Wound Repair. Adv Wound Care (New Rochelle). 2015 Apr 1;4(4):250-263. Rousselle P, Beck K. Laminin 332 processing impacts cellular behavior. Cell Adh Migr. 2013 Jan-Feb;7(1):122-34. doi: 10.4161/cam.23132. Senyürek I et al. Processing of laminin α chains generates peptides involved in woun d healing and host defense. J Innate Immun. 2014;6(4):467-84.
Editor's Notes
- LN (coloured circles), laminin N-terminal domain; LE, laminin-type epidermal growth factor-like repeats; L4/LF, laminin globular domains; LCC, laminin coiled coil; LG, laminin globular domains
- Laminin 5 deposits direct processive migration on collagen. Step 1.Deposition contacts: rapid switch of adhesion from exogenous collagen (blue) and integrin α2 (open ovals) to laminin 5 deposits (green ovals) and integrin α3β1 (red bars). Deposition of laminin 5 down onto the substratum is regulated differently than apical secretion of laminin 5, fibronectin, laminin 1 and thrombospondin into the culture medium. Step 2.Polarization: integrin α3-laminin 5 interactions mediate polarization. Focal complexes form under the lamellipodia with exogenous ligand while laminin 5 deposits are restricted to the rear of the cell. Step 3.Processive migration removes exogenous collagen at precise sites of laminin 5 deposits that are restricted to the rear of the cell. Persistence of linear migration is not in response to a chemotactic gradient.
- LN (coloured circles), laminin N-terminal domain; LE, laminin-type epidermal growth factor-like repeats; L4/LF, laminin globular domains; LCC, laminin coiled coil; LG, laminin globular domains