SlideShare a Scribd company logo

Characterization of the phi29 Bacteriophage Nanomotor

•Download as PPT, PDF•
•
P
pcpchic

The purpose is to present the evolution of our understanding to the Bacillus subtilis phi29 bacteriophage viral motor\'s structure based on the work of a prominent scientist, Peixuan Guo.

Technology
1 of 19
Characterization of the Bacteriophage Φ 29 Nanomotor May 1, 2009 Pearl C. Pfiester
Outline ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]
Purpose ,[object Object],[object Object],[object Object]
Figure 1. (Guo, Erickson, & Anderson, 1987) (A) Restriction maps of the left end of phi29 for restriction enzymes. (B) Restriction fragment designations of (C). (D) Autoradiograph of a Southern blot of fragments in (C) after hybridization with [32P]RNA probe that was purified from prohead. Discovering pRNA ,[object Object],[object Object]
Overview pRNA ? gp 16 gp 3
Number of Copies ,[object Object],[object Object]
Number of Copies ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],Figure 2. (Shu, Zhang, Jin, & Guo, 2007). Experimental set up for single-molecule imaging. (A) pRNA labeled with fluorescent label. (B) Experimental design for before initiation of DNA translocation and during translocation (C).
Number of Copies ,[object Object],[object Object],[object Object],[object Object],[object Object],Figure 3. (Shu, Zhang, Jin, & Guo, 2007). Dual-view imaging of procapsid/pRNA complexes containing dimers labeled Cy3 pRNA I and Cy5 pRNA II. (A) Dimer with both labels. (B) Fluorescence image of procapsids dual-labeled dimers. (C) Comparison of empirical and predicted distribution of photobleaching steps of Cy3 pRNA I. (D) Florescence intensity versus time to show photobleaching steps which shows each procapsid/pRNA complex had three Cy3 pRNA I and three Cy5 pRNA II.
Number of Copies Confirmed ,[object Object],[object Object],Figure 4. (Shu, Zhang, Jin, & Guo, 2007). Negatively stained EM images of pRNA conjugated with ferritin.
Structure of pRNA ,[object Object],Figure 5. (Chen, Sheng, Shao, & Guo, 2000). (I) Wild type of pRNA. (II) One of the circularly permutated pRNA or cpRNA of the right and left hand loops. (III) Hexameric pRNA formed by hand-in-hand interaction between loops from pRNA pairs.
Do pRNA pairs form dimers or trimers? ,[object Object],[object Object],Figure 6. (Chen, Sheng, Shao, & Guo, 2000). (A) Polyacrylamide gel showing monomers (M), dimers (D) and trimers (T) in lanes 1-10. Lanes 9 and 10 showed isolation of dimers and trimers and then subjected to 10% denaturing gels in lanes 11-13. (B) Purified dimers and trimers were compared after gradient sedimentation.
[object Object],Figure 7. (Chen, Sheng, Shao, & Guo, 2000). Cyro-AFM images of pRNA dimers, monomers, and trimers.
[object Object],[object Object],[object Object],[object Object],Figure 8. (Chen, Sheng, Shao, & Guo, 2000). Packaging activities of pRNA dimers.
Summary ,[object Object],[object Object],[object Object],[object Object],[object Object]
Proposed Models ,[object Object],[object Object],[object Object],[object Object],[object Object],See Movie: http://bilbo.bio.purdue.edu/~viruswww/Rossmann_home/movies/phi29rot.php
Proposed Models ,[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object],[object Object]
Nanomotor Innovation ,[object Object],[object Object],[object Object],[object Object],[object Object]
Literature Cited Chen C., Sheng S., Shao Z., & Guo P. (2000). A dimer as a building block in assembling RNA. Journal of Biological Chemistry . 23: 17510-17516. Guo P., Erickson S., & Anderson D. (1987). A small viral RNA is required for in vitro packaging of bacteriophage φ29 DNA. 236(4802): 690-694. Guo P. & Lee T. (2007). Viral nanomotors for packaging of dsDNA and dsRNA. Molecular Microbiology . 64(4): 886-903. Review Article. Shu D., Zhang H., Jin J., & Guo P. (2007). Counting of six pRNAs of phi29 DNA-packaging motor with customized single-molecule dual-view system. EMBO Journal . 26: 527-537.
Summary ,[object Object],[object Object],[object Object],[object Object],[object Object]

Recommended

Lecture2 nucleic acid (1)
Lecture2 nucleic acid (1)Lecture2 nucleic acid (1)
Lecture2 nucleic acid (1)SourabhKumar240
 
Role of dna polymerase delta
Role of dna polymerase deltaRole of dna polymerase delta
Role of dna polymerase deltaBudha Ratna RAV
 
Hoofdstuk 17 2008 deel 1
Hoofdstuk 17 2008 deel 1Hoofdstuk 17 2008 deel 1
Hoofdstuk 17 2008 deel 1guest29b928
 
Unit 1 transcription
Unit 1 transcriptionUnit 1 transcription
Unit 1 transcriptionDr. Mafatlal Kher
 
ASB PosterCompletepdf
ASB PosterCompletepdfASB PosterCompletepdf
ASB PosterCompletepdfKevin Loftiskg
 
Polymerases,
Polymerases,Polymerases,
Polymerases,Dr.M.Prasad Naidu
 
RNA structure and functions
RNA structure and functionsRNA structure and functions
RNA structure and functionsNamrata Chhabra
 
L10. enzymes used in genetic engineering i-1
L10. enzymes used in genetic engineering i-1L10. enzymes used in genetic engineering i-1
L10. enzymes used in genetic engineering i-1Rishabh Jain
 

Recommended

Lecture2 nucleic acid (1)
Lecture2 nucleic acid (1)Lecture2 nucleic acid (1)
Lecture2 nucleic acid (1)SourabhKumar240
 
Role of dna polymerase delta
Role of dna polymerase deltaRole of dna polymerase delta
Role of dna polymerase deltaBudha Ratna RAV
 
Hoofdstuk 17 2008 deel 1
Hoofdstuk 17 2008 deel 1Hoofdstuk 17 2008 deel 1
Hoofdstuk 17 2008 deel 1guest29b928
 
Unit 1 transcription
Unit 1 transcriptionUnit 1 transcription
Unit 1 transcriptionDr. Mafatlal Kher
 
ASB PosterCompletepdf
ASB PosterCompletepdfASB PosterCompletepdf
ASB PosterCompletepdfKevin Loftiskg
 
Polymerases,
Polymerases,Polymerases,
Polymerases,Dr.M.Prasad Naidu
 
RNA structure and functions
RNA structure and functionsRNA structure and functions
RNA structure and functionsNamrata Chhabra
 
L10. enzymes used in genetic engineering i-1
L10. enzymes used in genetic engineering i-1L10. enzymes used in genetic engineering i-1
L10. enzymes used in genetic engineering i-1Rishabh Jain
 
Cell physio 202
Cell physio 202Cell physio 202
Cell physio 202aaronpaulbaliga
 
(7) dental genetics
(7) dental genetics(7) dental genetics
(7) dental geneticsIAU Dent
 
Therapeutic gene modulation
Therapeutic gene modulationTherapeutic gene modulation
Therapeutic gene modulationErin Davis
 
Regulation of pten activity by its carboxyl terminal autoinhibitory
Regulation of pten activity by its carboxyl terminal autoinhibitoryRegulation of pten activity by its carboxyl terminal autoinhibitory
Regulation of pten activity by its carboxyl terminal autoinhibitoryChau Chan Lao
 
Presentation Protein Synthesis
Presentation Protein SynthesisPresentation Protein Synthesis
Presentation Protein Synthesisangelsalaman
 
Dna to rna to amino acids
Dna to rna to amino acidsDna to rna to amino acids
Dna to rna to amino acidsdrott22
 
Transposones
TransposonesTransposones
Transposonesmicrobiology Notes
 
Initiation and termination codons , mutation and genetic code
Initiation and termination codons , mutation and genetic codeInitiation and termination codons , mutation and genetic code
Initiation and termination codons , mutation and genetic codegohil sanjay bhagvanji
 
Rna structure 2019
Rna structure 2019Rna structure 2019
Rna structure 2019lalvarezmex
 
RNA Splicing
RNA SplicingRNA Splicing
RNA SplicingAmna Jalil
 
MOLECULAR ORGANIZATION OF EKARYOTIC RNA
MOLECULAR ORGANIZATION OF EKARYOTIC RNA MOLECULAR ORGANIZATION OF EKARYOTIC RNA
MOLECULAR ORGANIZATION OF EKARYOTIC RNAgohil sanjay bhagvanji
 
Bachelor Thesis Presentation
Bachelor Thesis PresentationBachelor Thesis Presentation
Bachelor Thesis PresentationMariya Licheva
 
Central dogma MCQ
Central dogma MCQCentral dogma MCQ
Central dogma MCQAfra Fathima
 
Second genetic code overlapping and split genes
Second genetic code overlapping and split genesSecond genetic code overlapping and split genes
Second genetic code overlapping and split genesgohil sanjay bhagvanji
 
Gene expression
Gene expressionGene expression
Gene expressionARPUTHA SELVARAJ A
 
LIGATION OF DNA
LIGATION OF DNALIGATION OF DNA
LIGATION OF DNAsaquib khan
 
Gutell 004.plasmid.1981.06.0112
Gutell 004.plasmid.1981.06.0112Gutell 004.plasmid.1981.06.0112
Gutell 004.plasmid.1981.06.0112Robin Gutell
 
Types of RNA
Types of RNATypes of RNA
Types of RNAKayeen Vadakkan
 
Prabhakar singh ii sem-paper-dna sequencing
Prabhakar singh ii sem-paper-dna sequencingPrabhakar singh ii sem-paper-dna sequencing
Prabhakar singh ii sem-paper-dna sequencingDepartment of Biochemistry, Veer Bahadur Singh Purvanchal Univarsity, Jaunpur
 
3.wall.transcription
3.wall.transcription3.wall.transcription
3.wall.transcriptionThumz Industries (MBBS)
 
nanotecnología
nanotecnologíananotecnología
nanotecnologíaconejo888g
 
A new methodology for a dramatic increase in efficiency of nanomotors of vary...
A new methodology for a dramatic increase in efficiency of nanomotors of vary...A new methodology for a dramatic increase in efficiency of nanomotors of vary...
A new methodology for a dramatic increase in efficiency of nanomotors of vary...shumikhm
 

More Related Content

What's hot

(7) dental genetics
(7) dental genetics(7) dental genetics
(7) dental geneticsIAU Dent
 
Therapeutic gene modulation
Therapeutic gene modulationTherapeutic gene modulation
Therapeutic gene modulationErin Davis
 
Regulation of pten activity by its carboxyl terminal autoinhibitory
Regulation of pten activity by its carboxyl terminal autoinhibitoryRegulation of pten activity by its carboxyl terminal autoinhibitory
Regulation of pten activity by its carboxyl terminal autoinhibitoryChau Chan Lao
 
Presentation Protein Synthesis
Presentation Protein SynthesisPresentation Protein Synthesis
Presentation Protein Synthesisangelsalaman
 
Dna to rna to amino acids
Dna to rna to amino acidsDna to rna to amino acids
Dna to rna to amino acidsdrott22
 
Initiation and termination codons , mutation and genetic code
Initiation and termination codons , mutation and genetic codeInitiation and termination codons , mutation and genetic code
Initiation and termination codons , mutation and genetic codegohil sanjay bhagvanji
 
Rna structure 2019
Rna structure 2019Rna structure 2019
Rna structure 2019lalvarezmex
 
MOLECULAR ORGANIZATION OF EKARYOTIC RNA
MOLECULAR ORGANIZATION OF EKARYOTIC RNA MOLECULAR ORGANIZATION OF EKARYOTIC RNA
MOLECULAR ORGANIZATION OF EKARYOTIC RNAgohil sanjay bhagvanji
 
Bachelor Thesis Presentation
Bachelor Thesis PresentationBachelor Thesis Presentation
Bachelor Thesis PresentationMariya Licheva
 
Second genetic code overlapping and split genes
Second genetic code overlapping and split genesSecond genetic code overlapping and split genes
Second genetic code overlapping and split genesgohil sanjay bhagvanji
 
Gutell 004.plasmid.1981.06.0112
Gutell 004.plasmid.1981.06.0112Gutell 004.plasmid.1981.06.0112
Gutell 004.plasmid.1981.06.0112Robin Gutell
 

What's hot (20)

Cell physio 202
Cell physio 202Cell physio 202
Cell physio 202
 
(7) dental genetics
(7) dental genetics(7) dental genetics
(7) dental genetics
 
Therapeutic gene modulation
Therapeutic gene modulationTherapeutic gene modulation
Therapeutic gene modulation
 
Regulation of pten activity by its carboxyl terminal autoinhibitory
Regulation of pten activity by its carboxyl terminal autoinhibitoryRegulation of pten activity by its carboxyl terminal autoinhibitory
Regulation of pten activity by its carboxyl terminal autoinhibitory
 
Presentation Protein Synthesis
Presentation Protein SynthesisPresentation Protein Synthesis
Presentation Protein Synthesis
 
Dna to rna to amino acids
Dna to rna to amino acidsDna to rna to amino acids
Dna to rna to amino acids
 
Transposones
TransposonesTransposones
Transposones
 
Initiation and termination codons , mutation and genetic code
Initiation and termination codons , mutation and genetic codeInitiation and termination codons , mutation and genetic code
Initiation and termination codons , mutation and genetic code
 
Rna structure 2019
Rna structure 2019Rna structure 2019
Rna structure 2019
 
RNA Splicing
RNA SplicingRNA Splicing
RNA Splicing
 
MOLECULAR ORGANIZATION OF EKARYOTIC RNA
MOLECULAR ORGANIZATION OF EKARYOTIC RNA MOLECULAR ORGANIZATION OF EKARYOTIC RNA
MOLECULAR ORGANIZATION OF EKARYOTIC RNA
 
Bachelor Thesis Presentation
Bachelor Thesis PresentationBachelor Thesis Presentation
Bachelor Thesis Presentation
 
Central dogma MCQ
Central dogma MCQCentral dogma MCQ
Central dogma MCQ
 
Second genetic code overlapping and split genes
Second genetic code overlapping and split genesSecond genetic code overlapping and split genes
Second genetic code overlapping and split genes
 
Gene expression
Gene expressionGene expression
Gene expression
 
LIGATION OF DNA
LIGATION OF DNALIGATION OF DNA
LIGATION OF DNA
 
Gutell 004.plasmid.1981.06.0112
Gutell 004.plasmid.1981.06.0112Gutell 004.plasmid.1981.06.0112
Gutell 004.plasmid.1981.06.0112
 
Types of RNA
Types of RNATypes of RNA
Types of RNA
 
Prabhakar singh ii sem-paper-dna sequencing
Prabhakar singh ii sem-paper-dna sequencingPrabhakar singh ii sem-paper-dna sequencing
Prabhakar singh ii sem-paper-dna sequencing
 
3.wall.transcription
3.wall.transcription3.wall.transcription
3.wall.transcription
 

Viewers also liked

nanotecnología
nanotecnologíananotecnología
nanotecnologíaconejo888g
 
A new methodology for a dramatic increase in efficiency of nanomotors of vary...
A new methodology for a dramatic increase in efficiency of nanomotors of vary...A new methodology for a dramatic increase in efficiency of nanomotors of vary...
A new methodology for a dramatic increase in efficiency of nanomotors of vary...shumikhm
 
Ferrofluid use in medicine
Ferrofluid use in medicineFerrofluid use in medicine
Ferrofluid use in medicineletmein1234567
 
Nano generators 242
Nano generators 242Nano generators 242
Nano generators 242Justin Rams
 
Nanogenerators, Producing Energy out of waste energy-The Future
Nanogenerators, Producing Energy out of waste energy-The Future Nanogenerators, Producing Energy out of waste energy-The Future
Nanogenerators, Producing Energy out of waste energy-The Future Siddhant Pathak
 
Nano generators
Nano generatorsNano generators
Nano generatorschakri218
 
Fluid mechanics in our daily life
Fluid mechanics in our daily lifeFluid mechanics in our daily life
Fluid mechanics in our daily lifeMehediHassanSourav
 
Nanogenerators
NanogeneratorsNanogenerators
NanogeneratorsParvathy M
 
Nanogenerator
Nanogenerator Nanogenerator
Nanogenerator Amit Kumar
 
Nanogenerator: Electricity with a pinch of your finger
Nanogenerator: Electricity with a pinch of your fingerNanogenerator: Electricity with a pinch of your finger
Nanogenerator: Electricity with a pinch of your fingerAKANKSHA SINGHAL
 
Magnetic nanoparticles applications and bioavailability for cancer therapy
Magnetic nanoparticles applications and bioavailability for cancer therapyMagnetic nanoparticles applications and bioavailability for cancer therapy
Magnetic nanoparticles applications and bioavailability for cancer therapyPravin Chinchole
 
Drug delivery and nanomedicine with nanomagnetic material
Drug delivery and nanomedicine with nanomagnetic materialDrug delivery and nanomedicine with nanomagnetic material
Drug delivery and nanomedicine with nanomagnetic materialshashank chetty
 
Nanotechnology and Fluid Flow Mechanics
Nanotechnology and Fluid Flow MechanicsNanotechnology and Fluid Flow Mechanics
Nanotechnology and Fluid Flow MechanicsVedant_09
 
Applications of fluid mechanics
Applications of fluid mechanicsApplications of fluid mechanics
Applications of fluid mechanicsVishu Sharma
 
Fluid mechanics applications
Fluid mechanics applicationsFluid mechanics applications
Fluid mechanics applicationsNofal Umair
 
Nanofluids PPT
Nanofluids PPT Nanofluids PPT
Nanofluids PPT Darshan J
 
Nano generator by Tanveer ahmed Ganganalli seminar ppt
Nano generator by Tanveer ahmed Ganganalli seminar pptNano generator by Tanveer ahmed Ganganalli seminar ppt
Nano generator by Tanveer ahmed Ganganalli seminar pptMD NAWAZ
 

Viewers also liked (20)

nanotecnología
nanotecnologíananotecnología
nanotecnología
 
A new methodology for a dramatic increase in efficiency of nanomotors of vary...
A new methodology for a dramatic increase in efficiency of nanomotors of vary...A new methodology for a dramatic increase in efficiency of nanomotors of vary...
A new methodology for a dramatic increase in efficiency of nanomotors of vary...
 
Ferrofluid use in medicine
Ferrofluid use in medicineFerrofluid use in medicine
Ferrofluid use in medicine
 
Ferrofluid v2
Ferrofluid v2Ferrofluid v2
Ferrofluid v2
 
Nano generators 242
Nano generators 242Nano generators 242
Nano generators 242
 
Ferrofluid
FerrofluidFerrofluid
Ferrofluid
 
Nanogenerators, Producing Energy out of waste energy-The Future
Nanogenerators, Producing Energy out of waste energy-The Future Nanogenerators, Producing Energy out of waste energy-The Future
Nanogenerators, Producing Energy out of waste energy-The Future
 
Nano generators
Nano generatorsNano generators
Nano generators
 
Fluid mechanics in our daily life
Fluid mechanics in our daily lifeFluid mechanics in our daily life
Fluid mechanics in our daily life
 
Nanogenerators
NanogeneratorsNanogenerators
Nanogenerators
 
Nanogenerator
Nanogenerator Nanogenerator
Nanogenerator
 
Nanogenerator: Electricity with a pinch of your finger
Nanogenerator: Electricity with a pinch of your fingerNanogenerator: Electricity with a pinch of your finger
Nanogenerator: Electricity with a pinch of your finger
 
Nanogenerator
NanogeneratorNanogenerator
Nanogenerator
 
Magnetic nanoparticles applications and bioavailability for cancer therapy
Magnetic nanoparticles applications and bioavailability for cancer therapyMagnetic nanoparticles applications and bioavailability for cancer therapy
Magnetic nanoparticles applications and bioavailability for cancer therapy
 
Drug delivery and nanomedicine with nanomagnetic material
Drug delivery and nanomedicine with nanomagnetic materialDrug delivery and nanomedicine with nanomagnetic material
Drug delivery and nanomedicine with nanomagnetic material
 
Nanotechnology and Fluid Flow Mechanics
Nanotechnology and Fluid Flow MechanicsNanotechnology and Fluid Flow Mechanics
Nanotechnology and Fluid Flow Mechanics
 
Applications of fluid mechanics
Applications of fluid mechanicsApplications of fluid mechanics
Applications of fluid mechanics
 
Fluid mechanics applications
Fluid mechanics applicationsFluid mechanics applications
Fluid mechanics applications
 
Nanofluids PPT
Nanofluids PPT Nanofluids PPT
Nanofluids PPT
 
Nano generator by Tanveer ahmed Ganganalli seminar ppt
Nano generator by Tanveer ahmed Ganganalli seminar pptNano generator by Tanveer ahmed Ganganalli seminar ppt
Nano generator by Tanveer ahmed Ganganalli seminar ppt
 

Similar to Characterization of the phi29 Bacteriophage Nanomotor

High throughput sequencing with Thermostable Group II Intron Reverse Transcri...
High throughput sequencing with Thermostable Group II Intron Reverse Transcri...High throughput sequencing with Thermostable Group II Intron Reverse Transcri...
High throughput sequencing with Thermostable Group II Intron Reverse Transcri...Douglas Wu
 
ShRNA-specific regulation of FMNL2 expression in P19 cells
ShRNA-specific regulation of FMNL2 expression in P19 cellsShRNA-specific regulation of FMNL2 expression in P19 cells
ShRNA-specific regulation of FMNL2 expression in P19 cellsYousefLayyous
 
Identification of Skeleton of Monoterpenoids from 13CNMR Data Using Generaliz...
Identification of Skeleton of Monoterpenoids from 13CNMR Data Using Generaliz...Identification of Skeleton of Monoterpenoids from 13CNMR Data Using Generaliz...
Identification of Skeleton of Monoterpenoids from 13CNMR Data Using Generaliz...IOSR Journals
 
Triplex dna 1
Triplex dna 1Triplex dna 1
Triplex dna 1CHAL91
 
Structure and genesis of mitochondrial and chloroplast, DNA replication , tra...
Structure and genesis of mitochondrial and chloroplast, DNA replication , tra...Structure and genesis of mitochondrial and chloroplast, DNA replication , tra...
Structure and genesis of mitochondrial and chloroplast, DNA replication , tra...Khalid Mukhtar
 
Summer 2015 REU Poster AK_FINAL
Summer 2015 REU Poster AK_FINALSummer 2015 REU Poster AK_FINAL
Summer 2015 REU Poster AK_FINALAndrew Kocian
 
High-throughput RNA sequencing with Thermostable Group II Intron Reverse Tran...
High-throughput RNA sequencing with Thermostable Group II Intron Reverse Tran...High-throughput RNA sequencing with Thermostable Group II Intron Reverse Tran...
High-throughput RNA sequencing with Thermostable Group II Intron Reverse Tran...Douglas Wu
 
New Generation Sequencing Technologies: an overview
New Generation Sequencing Technologies: an overviewNew Generation Sequencing Technologies: an overview
New Generation Sequencing Technologies: an overviewPaolo Dametto
 
Gutell 022.mbp.1992.52.0075
Gutell 022.mbp.1992.52.0075Gutell 022.mbp.1992.52.0075
Gutell 022.mbp.1992.52.0075Robin Gutell
 
New methods for high-throughput nucleic sequencing and diagnostics using a th...
New methods for high-throughput nucleic sequencing and diagnostics using a th...New methods for high-throughput nucleic sequencing and diagnostics using a th...
New methods for high-throughput nucleic sequencing and diagnostics using a th...Douglas Wu
 
Cpf1- a new tool for CRISPR genome editing
Cpf1- a new tool for CRISPR genome editingCpf1- a new tool for CRISPR genome editing
Cpf1- a new tool for CRISPR genome editingSachin Bhor
 
Molecular Biology Revision-Part1
Molecular Biology Revision-Part1Molecular Biology Revision-Part1
Molecular Biology Revision-Part1Namrata Chhabra
 
Candidacy Exam Final Version
Candidacy Exam Final VersionCandidacy Exam Final Version
Candidacy Exam Final VersionAnthony Salvagno
 
Use of TGIRT for ssDNA-seq
Use of TGIRT for ssDNA-seqUse of TGIRT for ssDNA-seq
Use of TGIRT for ssDNA-seqDouglas Wu
 

Similar to Characterization of the phi29 Bacteriophage Nanomotor (20)

High throughput sequencing with Thermostable Group II Intron Reverse Transcri...
High throughput sequencing with Thermostable Group II Intron Reverse Transcri...High throughput sequencing with Thermostable Group II Intron Reverse Transcri...
High throughput sequencing with Thermostable Group II Intron Reverse Transcri...
 
Gene expression
Gene expressionGene expression
Gene expression
 
ShRNA-specific regulation of FMNL2 expression in P19 cells
ShRNA-specific regulation of FMNL2 expression in P19 cellsShRNA-specific regulation of FMNL2 expression in P19 cells
ShRNA-specific regulation of FMNL2 expression in P19 cells
 
Identification of Skeleton of Monoterpenoids from 13CNMR Data Using Generaliz...
Identification of Skeleton of Monoterpenoids from 13CNMR Data Using Generaliz...Identification of Skeleton of Monoterpenoids from 13CNMR Data Using Generaliz...
Identification of Skeleton of Monoterpenoids from 13CNMR Data Using Generaliz...
 
Triplex dna 1
Triplex dna 1Triplex dna 1
Triplex dna 1
 
Structure and genesis of mitochondrial and chloroplast, DNA replication , tra...
Structure and genesis of mitochondrial and chloroplast, DNA replication , tra...Structure and genesis of mitochondrial and chloroplast, DNA replication , tra...
Structure and genesis of mitochondrial and chloroplast, DNA replication , tra...
 
CRISPR REPORT
CRISPR REPORTCRISPR REPORT
CRISPR REPORT
 
Summer 2015 REU Poster AK_FINAL
Summer 2015 REU Poster AK_FINALSummer 2015 REU Poster AK_FINAL
Summer 2015 REU Poster AK_FINAL
 
Rna
RnaRna
Rna
 
High-throughput RNA sequencing with Thermostable Group II Intron Reverse Tran...
High-throughput RNA sequencing with Thermostable Group II Intron Reverse Tran...High-throughput RNA sequencing with Thermostable Group II Intron Reverse Tran...
High-throughput RNA sequencing with Thermostable Group II Intron Reverse Tran...
 
New Generation Sequencing Technologies: an overview
New Generation Sequencing Technologies: an overviewNew Generation Sequencing Technologies: an overview
New Generation Sequencing Technologies: an overview
 
Gutell 022.mbp.1992.52.0075
Gutell 022.mbp.1992.52.0075Gutell 022.mbp.1992.52.0075
Gutell 022.mbp.1992.52.0075
 
GENE SEQUENCING
GENE SEQUENCINGGENE SEQUENCING
GENE SEQUENCING
 
New methods for high-throughput nucleic sequencing and diagnostics using a th...
New methods for high-throughput nucleic sequencing and diagnostics using a th...New methods for high-throughput nucleic sequencing and diagnostics using a th...
New methods for high-throughput nucleic sequencing and diagnostics using a th...
 
Cpf1- a new tool for CRISPR genome editing
Cpf1- a new tool for CRISPR genome editingCpf1- a new tool for CRISPR genome editing
Cpf1- a new tool for CRISPR genome editing
 
sequencing-methods-review
sequencing-methods-reviewsequencing-methods-review
sequencing-methods-review
 
Molecular Biology Revision-Part1
Molecular Biology Revision-Part1Molecular Biology Revision-Part1
Molecular Biology Revision-Part1
 
Candidacy Exam Final Version
Candidacy Exam Final VersionCandidacy Exam Final Version
Candidacy Exam Final Version
 
Use of TGIRT for ssDNA-seq
Use of TGIRT for ssDNA-seqUse of TGIRT for ssDNA-seq
Use of TGIRT for ssDNA-seq
 
Rnaseq forgenefinding
Rnaseq forgenefindingRnaseq forgenefinding
Rnaseq forgenefinding
 

Recently uploaded

08448380779 Call Girls In Diplomatic Enclave Women Seeking Men
08448380779 Call Girls In Diplomatic Enclave Women Seeking Men08448380779 Call Girls In Diplomatic Enclave Women Seeking Men
08448380779 Call Girls In Diplomatic Enclave Women Seeking MenDelhi Call girls
 
GenCyber Cyber Security Day Presentation
GenCyber Cyber Security Day PresentationGenCyber Cyber Security Day Presentation
GenCyber Cyber Security Day PresentationMichael W. Hawkins
 
2024: Domino Containers - The Next Step. News from the Domino Container commu...
2024: Domino Containers - The Next Step. News from the Domino Container commu...2024: Domino Containers - The Next Step. News from the Domino Container commu...
2024: Domino Containers - The Next Step. News from the Domino Container commu...Martijn de Jong
 
The Role of Taxonomy and Ontology in Semantic Layers - Heather Hedden.pdf
The Role of Taxonomy and Ontology in Semantic Layers - Heather Hedden.pdfThe Role of Taxonomy and Ontology in Semantic Layers - Heather Hedden.pdf
The Role of Taxonomy and Ontology in Semantic Layers - Heather Hedden.pdfEnterprise Knowledge
 
TrustArc Webinar - Stay Ahead of US State Data Privacy Law Developments
TrustArc Webinar - Stay Ahead of US State Data Privacy Law DevelopmentsTrustArc Webinar - Stay Ahead of US State Data Privacy Law Developments
TrustArc Webinar - Stay Ahead of US State Data Privacy Law DevelopmentsTrustArc
 
Tata AIG General Insurance Company - Insurer Innovation Award 2024
Tata AIG General Insurance Company - Insurer Innovation Award 2024Tata AIG General Insurance Company - Insurer Innovation Award 2024
Tata AIG General Insurance Company - Insurer Innovation Award 2024The Digital Insurer
 
Scaling API-first – The story of a global engineering organization
Scaling API-first – The story of a global engineering organizationScaling API-first – The story of a global engineering organization
Scaling API-first – The story of a global engineering organizationRadu Cotescu
 
Automating Google Workspace (GWS) & more with Apps Script
Automating Google Workspace (GWS) & more with Apps ScriptAutomating Google Workspace (GWS) & more with Apps Script
Automating Google Workspace (GWS) & more with Apps Scriptwesley chun
 
Injustice - Developers Among Us (SciFiDevCon 2024)
Injustice - Developers Among Us (SciFiDevCon 2024)Injustice - Developers Among Us (SciFiDevCon 2024)
Injustice - Developers Among Us (SciFiDevCon 2024)Allon Mureinik
 
Unblocking The Main Thread Solving ANRs and Frozen Frames
Unblocking The Main Thread Solving ANRs and Frozen FramesUnblocking The Main Thread Solving ANRs and Frozen Frames
Unblocking The Main Thread Solving ANRs and Frozen FramesSinan KOZAK
 
CNv6 Instructor Chapter 6 Quality of Service
CNv6 Instructor Chapter 6 Quality of ServiceCNv6 Instructor Chapter 6 Quality of Service
CNv6 Instructor Chapter 6 Quality of Servicegiselly40
 
Raspberry Pi 5: Challenges and Solutions in Bringing up an OpenGL/Vulkan Driv...
Raspberry Pi 5: Challenges and Solutions in Bringing up an OpenGL/Vulkan Driv...Raspberry Pi 5: Challenges and Solutions in Bringing up an OpenGL/Vulkan Driv...
Raspberry Pi 5: Challenges and Solutions in Bringing up an OpenGL/Vulkan Driv...Igalia
 
The Codex of Business Writing Software for Real-World Solutions 2.pptx
The Codex of Business Writing Software for Real-World Solutions 2.pptxThe Codex of Business Writing Software for Real-World Solutions 2.pptx
The Codex of Business Writing Software for Real-World Solutions 2.pptxMalak Abu Hammad
 
Salesforce Community Group Quito, Salesforce 101
Salesforce Community Group Quito, Salesforce 101Salesforce Community Group Quito, Salesforce 101
Salesforce Community Group Quito, Salesforce 101Paola De la Torre
 
Workshop - Best of Both Worlds_ Combine KG and Vector search for enhanced R...
Workshop - Best of Both Worlds_ Combine KG and Vector search for enhanced R...Workshop - Best of Both Worlds_ Combine KG and Vector search for enhanced R...
Workshop - Best of Both Worlds_ Combine KG and Vector search for enhanced R...Neo4j
 
The 7 Things I Know About Cyber Security After 25 Years | April 2024
The 7 Things I Know About Cyber Security After 25 Years | April 2024The 7 Things I Know About Cyber Security After 25 Years | April 2024
The 7 Things I Know About Cyber Security After 25 Years | April 2024Rafal Los
 
Boost PC performance: How more available memory can improve productivity
Boost PC performance: How more available memory can improve productivityBoost PC performance: How more available memory can improve productivity
Boost PC performance: How more available memory can improve productivityPrincipled Technologies
 
IAC 2024 - IA Fast Track to Search Focused AI Solutions
IAC 2024 - IA Fast Track to Search Focused AI SolutionsIAC 2024 - IA Fast Track to Search Focused AI Solutions
IAC 2024 - IA Fast Track to Search Focused AI SolutionsEnterprise Knowledge
 
Driving Behavioral Change for Information Management through Data-Driven Gree...
Driving Behavioral Change for Information Management through Data-Driven Gree...Driving Behavioral Change for Information Management through Data-Driven Gree...
Driving Behavioral Change for Information Management through Data-Driven Gree...Enterprise Knowledge
 
[2024]Digital Global Overview Report 2024 Meltwater.pdf
[2024]Digital Global Overview Report 2024 Meltwater.pdf[2024]Digital Global Overview Report 2024 Meltwater.pdf
[2024]Digital Global Overview Report 2024 Meltwater.pdfhans926745
 

Recently uploaded (20)

08448380779 Call Girls In Diplomatic Enclave Women Seeking Men
08448380779 Call Girls In Diplomatic Enclave Women Seeking Men08448380779 Call Girls In Diplomatic Enclave Women Seeking Men
08448380779 Call Girls In Diplomatic Enclave Women Seeking Men
 
GenCyber Cyber Security Day Presentation
GenCyber Cyber Security Day PresentationGenCyber Cyber Security Day Presentation
GenCyber Cyber Security Day Presentation
 
2024: Domino Containers - The Next Step. News from the Domino Container commu...
2024: Domino Containers - The Next Step. News from the Domino Container commu...2024: Domino Containers - The Next Step. News from the Domino Container commu...
2024: Domino Containers - The Next Step. News from the Domino Container commu...
 
The Role of Taxonomy and Ontology in Semantic Layers - Heather Hedden.pdf
The Role of Taxonomy and Ontology in Semantic Layers - Heather Hedden.pdfThe Role of Taxonomy and Ontology in Semantic Layers - Heather Hedden.pdf
The Role of Taxonomy and Ontology in Semantic Layers - Heather Hedden.pdf
 
TrustArc Webinar - Stay Ahead of US State Data Privacy Law Developments
TrustArc Webinar - Stay Ahead of US State Data Privacy Law DevelopmentsTrustArc Webinar - Stay Ahead of US State Data Privacy Law Developments
TrustArc Webinar - Stay Ahead of US State Data Privacy Law Developments
 
Tata AIG General Insurance Company - Insurer Innovation Award 2024
Tata AIG General Insurance Company - Insurer Innovation Award 2024Tata AIG General Insurance Company - Insurer Innovation Award 2024
Tata AIG General Insurance Company - Insurer Innovation Award 2024
 
Scaling API-first – The story of a global engineering organization
Scaling API-first – The story of a global engineering organizationScaling API-first – The story of a global engineering organization
Scaling API-first – The story of a global engineering organization
 
Automating Google Workspace (GWS) & more with Apps Script
Automating Google Workspace (GWS) & more with Apps ScriptAutomating Google Workspace (GWS) & more with Apps Script
Automating Google Workspace (GWS) & more with Apps Script
 
Injustice - Developers Among Us (SciFiDevCon 2024)
Injustice - Developers Among Us (SciFiDevCon 2024)Injustice - Developers Among Us (SciFiDevCon 2024)
Injustice - Developers Among Us (SciFiDevCon 2024)
 
Unblocking The Main Thread Solving ANRs and Frozen Frames
Unblocking The Main Thread Solving ANRs and Frozen FramesUnblocking The Main Thread Solving ANRs and Frozen Frames
Unblocking The Main Thread Solving ANRs and Frozen Frames
 
CNv6 Instructor Chapter 6 Quality of Service
CNv6 Instructor Chapter 6 Quality of ServiceCNv6 Instructor Chapter 6 Quality of Service
CNv6 Instructor Chapter 6 Quality of Service
 
Raspberry Pi 5: Challenges and Solutions in Bringing up an OpenGL/Vulkan Driv...
Raspberry Pi 5: Challenges and Solutions in Bringing up an OpenGL/Vulkan Driv...Raspberry Pi 5: Challenges and Solutions in Bringing up an OpenGL/Vulkan Driv...
Raspberry Pi 5: Challenges and Solutions in Bringing up an OpenGL/Vulkan Driv...
 
The Codex of Business Writing Software for Real-World Solutions 2.pptx
The Codex of Business Writing Software for Real-World Solutions 2.pptxThe Codex of Business Writing Software for Real-World Solutions 2.pptx
The Codex of Business Writing Software for Real-World Solutions 2.pptx
 
Salesforce Community Group Quito, Salesforce 101
Salesforce Community Group Quito, Salesforce 101Salesforce Community Group Quito, Salesforce 101
Salesforce Community Group Quito, Salesforce 101
 
Workshop - Best of Both Worlds_ Combine KG and Vector search for enhanced R...
Workshop - Best of Both Worlds_ Combine KG and Vector search for enhanced R...Workshop - Best of Both Worlds_ Combine KG and Vector search for enhanced R...
Workshop - Best of Both Worlds_ Combine KG and Vector search for enhanced R...
 
The 7 Things I Know About Cyber Security After 25 Years | April 2024
The 7 Things I Know About Cyber Security After 25 Years | April 2024The 7 Things I Know About Cyber Security After 25 Years | April 2024
The 7 Things I Know About Cyber Security After 25 Years | April 2024
 
Boost PC performance: How more available memory can improve productivity
Boost PC performance: How more available memory can improve productivityBoost PC performance: How more available memory can improve productivity
Boost PC performance: How more available memory can improve productivity
 
IAC 2024 - IA Fast Track to Search Focused AI Solutions
IAC 2024 - IA Fast Track to Search Focused AI SolutionsIAC 2024 - IA Fast Track to Search Focused AI Solutions
IAC 2024 - IA Fast Track to Search Focused AI Solutions
 
Driving Behavioral Change for Information Management through Data-Driven Gree...
Driving Behavioral Change for Information Management through Data-Driven Gree...Driving Behavioral Change for Information Management through Data-Driven Gree...
Driving Behavioral Change for Information Management through Data-Driven Gree...
 
[2024]Digital Global Overview Report 2024 Meltwater.pdf
[2024]Digital Global Overview Report 2024 Meltwater.pdf[2024]Digital Global Overview Report 2024 Meltwater.pdf
[2024]Digital Global Overview Report 2024 Meltwater.pdf
 

Characterization of the phi29 Bacteriophage Nanomotor

  • 1. Characterization of the Bacteriophage Φ 29 Nanomotor May 1, 2009 Pearl C. Pfiester
  • 2.
  • 3.
  • 4.
  • 5. Overview pRNA ? gp 16 gp 3
  • 6.
  • 7.
  • 8.
  • 9.
  • 10.
  • 11.
  • 12.
  • 13.
  • 14.
  • 15.
  • 16.
  • 17.
  • 18. Literature Cited Chen C., Sheng S., Shao Z., & Guo P. (2000). A dimer as a building block in assembling RNA. Journal of Biological Chemistry . 23: 17510-17516. Guo P., Erickson S., & Anderson D. (1987). A small viral RNA is required for in vitro packaging of bacteriophage φ29 DNA. 236(4802): 690-694. Guo P. & Lee T. (2007). Viral nanomotors for packaging of dsDNA and dsRNA. Molecular Microbiology . 64(4): 886-903. Review Article. Shu D., Zhang H., Jin J., & Guo P. (2007). Counting of six pRNAs of phi29 DNA-packaging motor with customized single-molecule dual-view system. EMBO Journal . 26: 527-537.
  • 19.

Editor's Notes

  1. I would like to thank Dr. Visalli for mentoring me throughout this project. Today I’m going to discuss the Characterization of the bacteriophage phi29 nanomotor.