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Genetic Variability, Heritability and Genetic Advance in Potato (Solanum Tuberosum L.) for Processing Quality, Yield and Yield Related Traits
Genetic Variability, Heritability and Genetic Advance in
Potato (Solanum Tuberosum L.) for Processing Quality,
Yield and Yield Related Traits
Ebrahim Seid1*, Wassu Mohammed2 and Tessfaye Abebe1
1Ethiopia Institute of Agricultural Research, Holetta Agricultural Research Centre, P. O. Box: 2003, Addis Ababa, Ethiopia.
2Haramaya University, P.O.Box 138, Dire Dawa, Ethiopia.
Potato (Solanum tuberosum L.) used as fresh products and commercially processed foods
such as French fries and chips.The objective of the experiment was to assess the nature and
magnitude of variability in potato genotypes for tuber quality, yield and yield-related traits.
Twenty four potato genotypes were evaluated at Holetta Agricultural Research Centre using a
randomized complete block design with three replications during the growing season of 2017.
The results of the analysis of variance indicated there was highly significant differences among
the genotypes for all traits excepted peel content. The phenotypic (PCV) and genotypic (GCV)
coefficient of variation ranged between 0.90 to 46.43% and 0.75 to 40.0%. Heritability in the
broad sense (H2
) and genetic advance as percent of the mean (GAM) ranged from 38.13 to
91.64% and 1.28 to 73.50%. High phenotypic coefficients of variation and genotypic coefficients
of variation coupled with high heritability and genetic advance as percent of mean were
observed for shoot dry mass weight, average tuber number, average tuber weight,
unmarketable tuber yield, small size tuber and large size tubers. Therefore, selection for these
characters would be effective for the emerging processing industry and could be selected as
parents for future crossing program in Ethiopia.
Keywords: Tuber quality, potato variability, genotypic variation, phenotypic variation, co-efficient of variance
INTRODUCTION
Potato (Solanum tuberosum L.) is the third most important
food crop in the world after rice and wheat in terms of
human consumption. More than a billion people eat potato
worldwide, and global total production exceeds 300 million
metric tons. Ther are more than 4,000 varieties of native
potato and also over 180 wild species, mostly found in the
Andes (CIP, 2020). In central highlands Ethiopia an
adaptation trial of potato was conducted to identify potato
varieties that is better to adaptation, yield and other
agronomic traits and disease tolerant. The national
average potato yield in Ethiopia is 13.9 t ha-1 (CSA., 2018),
which is lower than the experimental yield of over 35 t ha -
1 (Berihun, 2013), and world average yield to 20 t ha-1
(FAOSTAT., 2019). It is a nutritious vegetable containing
16% carbohydrates, 2% proteins, 1% minerals, 0.6%
dietary fiber and a negligible amount of fat (Gumul et al.,
2011). In Eastern African potato is the high yield potential
and plasticity to environmental regimes makes it on of the
best crops for food and nutrition security (Kyamanywa et
al., 2011). The large proportion (60 to 80%) of dry matter
is composed of starches from 20% of the average dry
matter content, it is a food rich in carbohydrate (Lutaladio
and Castaidi, 2009). Besides being a rich source of
carbohydrates, potato also contains some health-
promoting compounds such as phenolic acids, ascorbic
acid and carotenoids (Ezekiel et al., 2013).
*Corresponding Author: Ebrahim Seid; Ethiopia Institute
of Agricultural Research, Holetta Agricultural Research
Centre, P. O. Box: 2003, Addis Ababa, Ethiopia
*1
Corresponding Author Email:
ibrahussen32@gmail.com
2
Co-Author Email: wasmoha@yahoo.com
1
Co-author Email: destaadera@gmail.com
International Journal of Plant Breeding and Crop Science
Vol. 7(3), pp. 928-936, November, 2020. © www.premierpublishers.org, ISSN: 2167-0449
Research Article
Genetic Variability, Heritability and Genetic Advance in Potato (Solanum Tuberosum L.) for Processing Quality, Yield and Yield Related Traits
Ebrahim et al. 929
The genetic makeup, crop maturity, agronomic practices,
environmental conditions, storage temperatures, pests
and diseases are affecting tuber. Studying the genetic
variability present among different potato genotypes for a
given character is a basic precondition to design
systematic breeding methods. Predominantly, genetic
variability for a given character is a basic precondition for
its improvement by systematic breeding (Engida et al.,
2007; Arslanoglu et al., 2011).
Strategic potato research in Ethiopia began in 1975 with
the understanding of the constraints challenging its
production and productivity (Baye and Gebremedhin,
2013). The development and dissemination of more than
36 ( Belete, Gudanie, Dagim and Jalene…) improved
varieties, coupled with other technological packages,
contributed greatly to the improvement and rapid
expansion in potato production (MANR, 2016). The major
objective of potato breeding has been to develop potato
cultivars that have maximum yield potential, adaptable to
wide agro-ecologies and resistant to late blight that has
been the most devastating disease throughout the
dominant potato producing highlands of the country
(Wassu, 2016). Few studies have been conducted on
processing quality potato genotypes (Elfnesh et al., 2011;
Tesfaye et al., 2012; Wassu, 2016; Wassu, 2017).
However, the released varieties have not satisfied the
consumer for French fries and chips making process
(Habtamu et al., 2016). Therefore, it is necessary to
conduct a comprehensive study on genetic variability of
available genotype for tuber quality, yield and yield-related
traits and document information on the available varieties
quality features. The present experiment was conducted to
assess the nature and magnitude of variability in potato
genotypes for tuber quality, yield and yield-related traits
with the help of genetic parameters, such as phenotypic as
well as genotypic coefficient of variation and genotypic
coefficients of variation and estimate of heritability in the
broad sense.
MATERIALS AND METHODS
Experimental Site, Design and Materials
A total of 24 potato genotypes were used for the
experiment. These included 21 genotypes and three
released varieties (Table 1). The 24 genotypes were
planted at Holleta Agricultural Research Centre
experiment station during the main cropping season of
2017. The experiment was laid out in randomized
complete block design (RCBD) with three replications and
each plot was 3.6 m (length) x 4.5 m (width) (16.2 m2 gross
plot size) consisted six rows each containing 12 plants and
thus 72 plants per plot. The spacing between rows and
plants was 0.75 m and 0.30 m, respectively. The spacing
between plots and adjacent replications was 1 m and 1.5
m, respectively.
Table 1: List of experimental materials included in the
study
No. Accession code No. Accession code
1 CIP-396034.268 13 CIP-394611.112
2 CIP-393220.54 14 CIP-392617.54
3 CIP-395017.229 15 CIP-381381.20
4 CIP-392797.27 16 CIP-398180.289
5 CIP-395112.19 17 CIP-.398190.89
6 CIP-399075.7 18 CIP-398190.404
7 CIP-393280.64 19 CIP-391058.175
8 CIP-398098.65 20 CIP-396034.103
9 CIP-393385.39 21 CIP-391046.14
10 CIP-396027.205 22 Belete (CIP-393371.58)
11 CIP-393077.159 23 Gudanie (CIP-386423.13)
12 CIP-399002.52 24 Dagim (CIP-396004.337)
CIP = International Potato Center
Data Collection
Phenology and Growth Parameters
Data was recorded for phenology and growth parameters;
days to 50% flowering, days to maturity, plant height (cm),
average stems number and leaf area index (cm-3).
Yield and Yield Components
Data was recorded for yield parameters; shoot dry mass
weight (g), tubers dry mass weight (g): total biomass
weight (g), average tuber number /hill, average tuber
weight (g/tuber), tuber size distribution:- small (< 35mm),
medium (35 to 50mm), and large (>50 mm) size tubers
(%), total tuber yield (t/ha), marketable tuber yield(/ha) and
unmarketable tuber yield(t/ha). The amount of tuber
number in different size categories was changed to a
percentage (Ekin et al., 2009).
Tuber Physical and Internal Quality Traits
Peel content (%): Ten fresh tubers were randomly
selected from each plot, weight and peeled. The peel of
the tubers was weighted. The mean weight of single tuber
and peel content was recorded and then expressed as a
percentage as follows:
Peel content (%) =
tuberofWeight
contentpeel
x 100
Geometric mean diameter (Dg) (mm): The sizes of ten
randomly selected tubers from each plot were measured
as length, width and thickness using a digital caliper with
an accuracy of 0.01mm. The following equation calculated
the geometric mean diameter (Dg):
Dg= (LWT) 0.333 where: L is length; W is width and T is the
thickness of the tuber.
Genetic Variability, Heritability and Genetic Advance in Potato (Solanum Tuberosum L.) for Processing Quality, Yield and Yield Related Traits
Int. J. Plant Breed Crop Sci. 930
Length to width ratio: Recorded as the ratio of tubers
length to width and then expressed in terms ratio.
Sphericity of tuber () (%): the sphericity was
determined by the following formula as described by
Ahmadi et al. (2008):  = (Dg/L) x 100
Where,  is the sphercity of the tuber, Dg is the geometric
mean diameter and L is the length
Surface area (S) (mm2
): Tubers surface area was
determined according to Bareh (2000) by the following
formula: S =  Dg2 where: S is surface area and Dg is the
geometric mean diameter
Specific gravity of tubers (Sg) (gcm-3
): It was
determined using the weight in air/weight in water
methods. Five kilogram tubers of all shapes and sizes
were randomly taken from each plot. The selected tubers
were washed with water. First, it was weight in air and then
re-weighed suspended in water and the specific gravity
was determined according to the following formula (Gould,
1995).
Specific gravity =
weight in air
weight in air − weight in water
Dry matter content (%): The total dry matter content
(DMC) was calculated according to Porras et al. (2014).
Five tubers of each plot were chopped (about 500g total)
into small 1-2 cm cubes. They were mixed thoroughly and
two subsamples of 200g each were taken. The exact
weight of each subsample was recorded as fresh weight.
Consequently, each subsample was placed in an oven set
at 80°C for 48 hours and dried until constant weight. Each
subsample were weighted immediately and recorded as
dry weight. The dry matter content for each subsample
was then computed with the following formula.
Dry matter content (%) =
dry weight
fresh weight
∗ 100
Total starch content (g/100g): This was estimated from
dry matter content. Total starch content (g/100g): This was
estimated from dry matter. Starch content (%) = 17.55 +
0.891* ( tuber dry weight % - 24.182) AOAC, 1980) where
the dry matter was determined as indicated above it was
measured from tubers of the five randomly selected plants
to be used for tuber dry mass estimation was sliced and
kept in an oven at 80°C for 48 hours and weighed after
cooling in room temperature.
Data Analysis
Analysis of variance
The data was subjected to analysis of variance (ANOVA)
using the SAS statistical software 9.2 (SAS, 2008). The
comparison of the mean performance of genotypes was
done the significance of mean squares using Duncan’s
multiple range test (DMRT). The traits that exhibited
significant mean squares in general ANOVA was further
subjected to genetic analyses.
Phenotypic and Genotypic Variability
The genotypic and phenotypic variability of each
quantitative traits was estimated as genotypic and
phenotypic variances and coefficients of variation. The
genotypic and phenotypic coefficient of variation was
determined by the formula as described by Burton and de
Vane (1953)
Genotypic variance (σ2g) =
𝑀𝑆𝑔−𝑀𝑆𝑒
𝑟
Where, σ2g = genotypic variance, MSg = mean square
of genotype, MSe = mean square of error, r = number
of replications and environmental variance (𝜎2 𝑒) =
mean square of the error
Phenotypic variance (σ2p) = σ2g + σ2e
Where, σ2g = genotypic variance, σ2e = Environmental
variance and σ2p = Phenotypic variance
100
x
_
2
p
PCV 











 And 100_
2












x
g
GCV

Where: PCV= Phenotypic coefficient of variation,
GCV= Genotypic coefficient of variation and 𝐱 =
population mean of the character being evaluated
PCV and GCV values were categorized as low,
moderate, and high values, as indicated by
Sivasubramaniah and Menon (1973) as follows.
0-10% = low, 10-20% moderate and also > 20% = High
Heritability and Genetic Advance
Broad sense heritability values were estimated using
the formula adopted by Falconer and Mackay (1996) as
follows:
H2 = (σ2g/σ2p) x 100
Where, H2 = heritability in broad sense, σ2p =
Phenotypic variance and σ2g = genotypic variance
The heritability percentage was categorized as low,
moderate and high, as suggested by Robinson and
Callbeck (1955).
0-30% = low, 30-60% = moderate and > 60% = high
Expected genetic advance under selection (GA):
Expected genetic advance (GA) at 5% percent
selection intensity and genetic advance as percentage
of mean (GAM) was calculated through the method of
Johnson et al. (1955)
GA = K*SDp*H2
Where, GA = Genetic advance, SDp = phenotypic
standard deviation on mean basis, H2 = Heritability in
the broad sense and K = standardized selection
differential at 5% selection intensity (K = 2.063)
Genetic Variability, Heritability and Genetic Advance in Potato (Solanum Tuberosum L.) for Processing Quality, Yield and Yield Related Traits
Ebrahim et al. 931
Genetic advance as percent of the mean (GAM)
Genetic advance as percent of mean was estimated as
follows:
GAM =
GA
X
X100
Where GAM = Genetic advance as percent of the mean,
GA = genetic advance and
X = Population mean of the character being evaluated
The magnitude of genetic advance as percentage of
mean was categorized as low (0-10%), moderate (10-
20%), and high (> 20%), as suggested by Johanson et
al. (1955).
RESULT AND DISCUSSION
Analysis of Variance
The results of the analysis of variance indicated the
presence of highly significant (p<0.01) differences among
the tested potato genotypes for all traits excepted peel
content (Table 2). This will provide a good opportunity for
the breeder to select genotypes of varied maturity groups,
tuber yield performance and traits related to processing
aspects. Several workers, Misgana et al. (2015);
Getachew et al. (2016); Habtamu et al. (2016); Wassu
(2016); Wassu (2017) reported highly significant
differences among genotypes, for phenology, tuber yield
and processing quality traits.
Table 2: Mean squares from analysis of growth parameters yield and quality-related traits and yield of 24 potato
genotypes evaluated at Holetta in 2017.
Traits Mean Replication (2) Genotype (23) Error (46) CV (%)
Days to 50% flowering 54.19 7.51 54.72** 1.82 2.49
Days to maturity 95.92 18.38 117.86** 13.04 3.77
Plant height (cm) 80.48 385.17 495.62** 14.62 4.75
Average stems number 4.05 0.67 1.86** 0.24 12.06
Leaf area index 2.56 0.17 0.55** 0.05 8.95
Shoot dry mass weight (g/plant) 46.00 145.82 617.52** 69.66 18.14
Tuber dry mass weight (g/plant) 166.14 497.39 1918.32** 558.03 14.22
Total biomass weight (g/plant) 212.14 593.79 2790.60** 723.03 12.68
Average tuber number per hill 11.08 12.49 16.43** 1.58 11.34
Average tuber weight (g/tuber) 66.36 15.19 640.17** 34.77 8.89
Small size tubers (%) 34.12 259.42 248.00** 41.86 18.96
Medium size tubers (%) 39.93 143.46 105.25** 30.34 13.79
Large size tubers (%) 25.95 30.79 351.23** 28.03 20.41
Total tuber yield (t/ha) 31.63 59.35 91.85** 11.70 10.81
Marketable tuber yield (t/ha) 28.74 42.02 75.09** 11.65 11.88
Unmarkatable tuber yield (t/ha) 2.89 1.63 4.37** 0.52 24.82
Peel content (%) 14.85 5.73 4.73ns 3.41 12.39
Geometric mean diameter (mm3) 55.60 13.19 37.24** 13.07 6.50
Sphericity of the tuber (%) 80.20 0.01 179.79** 7.20 3.35
Surface area (mm2) 9772.71 1626891.6 4480789.00** 1552507.4 12.75
Length to width ratio 1.27 0.002 0.122** 0.005 5.64
Specific gravity of tubers (g/cm3) 1.09 0.00000768 0.00022599** 0.00002919 0.50
Dry matter content (%) 21.94 0.34 10.83** 1.39 5.37
Total starch content (g/100g) 15.55 0.26 8.60** 1.10 6.76
** - Significant at P<0.01 and ns - non-significant, number in parenthesis represented degree of freedom for the respective
source of variation, cm = centimeter, g= gram, t/ha= ton per hectare, mm3 = millimeter cub, mm2 = millimeter square and
g/cm3 = gram per centimeter cub
Genetic Variability, Heritability and Genetic Advance in Potato (Solanum Tuberosum L.) for Processing Quality, Yield and Yield Related Traits
Estimates of Variability Components
Phenotypic and Genotypic Variation
The phenotypic coefficient of variation (PCV) and
genotypic coefficient of variation (GCV) ranged from 0.90
to 46.43% and 0.75 to 40.0%, respectively (Table 3).
Sivasubramaniah and Menon (1973) categorized PCV and
GCV coefficient of variation values low (<10%), moderate
(10-20), and high (>20%). Based on these categories,
days to 50% flowering, days to maturity, geometric mean
diameter, sphericity of the tuber, specific gravity of tuber
and dry matter content showed low PCV and GCV except
sphericity the tuber that had moderate PCV. Plant height,
average stems number, leaf area index, tubers dry mass
weight, total biomass weight, total tuber yield, marketable
tuber yield, medium size tuber, surface area, length to
width ratio and total starch content had both moderate
PCV and GCV values except average stems number that
had high PCV values.
Shoot dry mass weight, average tuber number, average
tuber weight, unmarketable tuber yield, small size tubers
and large size tuber traits had high PCV and GCV values.
The high PCV and GCV indicated that the traits are
controlled by genetic factors and hence there is a higher
chance of improvement of the crop through selection.
Singh (1990) suggested that traits that exhibited high
estimates of GCV and PCV have a high probability of
improvement through selection. However, the author
indicated the improvement of traits is difficult and
impractical through selection for traits that exhibited low
estimates for both variability components due to the
masking effect of the environment on the genotypic effect.
Int. J. Plant Breed Crop Sci. 932
A similar study carried by Regassa and Basavaraj (2005);
Addisu et al. (2013); Getachew et al. (2016); Tripura et al.
(2016); Panigrahi et al. (2017) observed high GCV and
PCV for average tuber number, average tuber weight,
small size tubers, large size tubers and unmarketable
tuber yield. Ummyiah et al. (2010) reported moderate PCV
and GCV for total tuber yield and low PCV and GCV for
specific gravity and dry matter content. Ara et al. (2009),
Mishra et al. (2017) reported moderate PCV and GCV for
total starch content, shoot dry mass weight and average
stems number.
Genetic Variability, Heritability and Genetic Advance in Potato (Solanum Tuberosum L.) for Processing Quality, Yield and Yield Related Traits
Ebrahim et al. 933
Table 3: Estimate of variability components for 23 traits in 24 potato genotypes evaluated at Holetta in 2017
σ2g σ2p σ2e PCV (%) GCV (%) H2 (%) GA GAM (5 %) Difference
between PCV &
GCV
Days to 50% flowering 17.63 19.45 1.82 8.14 7.75 90.65 8.25 15.22 0.39
Days to maturity 34.94 47.98 13.04 7.22 6.16 72.82 10.41 10.85 1.06
Plant height (cm) 160.33 174.95 14.62 16.44 15.73 91.64 25.01 31.07 0.71
Average stems number 0.54 0.78 0.24 21.81 18.17 69.43 1.27 31.24 3.64
Leaf area index 0.17 0.22 0.05 18.30 15.97 76.10 0.74 28.73 2.33
Shoot dry mass weight (g/plant) 182.62 252.28 69.66 34.53 29.38 72.39 23.72 51.56 5.15
Tubers dry mass weight (g/plant) 453.43 1011.46 558.03 19.14 12.82 44.83 29.41 17.70 6.32
Total biomass weight (g/plant) 689.19 1412.23 723.04 17.71 12.38 48.80 37.83 17.83 5.33
Average tuber number per hill 4.95 6.53 1.58 23.06 20.08 75.80 4.00 36.06 2.98
Average tuber weight (g/tuber) 201.80 236.57 34.77 23.18 21.41 85.30 27.07 40.79 1.77
Small size tubers (%) 68.71 110.57 41.86 30.82 24.29 62.14 13.48 39.51 6.53
Medium size tubers (%) 24.97 55.31 30.34 18.62 12.51 45.15 6.93 17.35 6.11
Large size tubers (%) 107.73 135.77 28.03 44.90 40.00 79.35 19.07 73.50 4.90
Total tuber yield (t/ha) 26.72 38.42 11.70 19.60 16.34 69.54 8.89 28.12 3.26
Marketable tuber yield (t/ha) 21.14 32.80 11.65 19.93 16.00 64.47 7.62 26.50 3.93
Unmarketable tuber yield (t/ha) 1.29 1.80 0.52 46.43 39.23 71.41 1.98 68.39 7.20
Geometric mean diameter (mm3) 8.06 21.13 13.07 8.27 5.10 38.13 3.62 6.50 3.17
Sphericity of the tuber (%) 57.53 64.73 7.20 10.03 9.46 88.87 14.75 18.39 0.57
Surface area (mm2) 976093.87 2528601.27 1552507.40 16.27 10.11 38.60 1266.34 12.96 6.16
Length to width ratio 0.04 0.04 0.01 16.52 15.52 82.32 0.38 30.10 1.00
Specific gravity of tubers (g/cm3) 0.0000656 0.0000948 0.00002919 0.90 0.75 69.21 0.01 1.28 0.15
Dry matter content (%) 3.15 4.54 1.39 9.71 8.09 69.40 3.05 13.90 1.62
Total starch content (g/100g) 2.50 3.60 1.10 12.20 10.16 69.35 2.72 17.46 2.04
σ2g = genotypic variance, σ2p = phenotypic variance. σ2e = environment variance, PCV = phenotypic coefficient of variation, GCV = genotypic coefficient, H2 =
heritability in broad sense, GA = genetic advance, GAM = genetic advance as percent of mean, cm = centimeter, g= gram, t/ha= ton per hectare, mm3 = millimeter
cub, mm2 = millimeter square and g/cm3 = gram per centimeter cub
Genetic Variability, Heritability and Genetic Advance in Potato (Solanum Tuberosum L.) for Processing Quality, Yield and Yield Related Traits
Int. J. Plant Breed Crop Sci. 934
Estimate of Heritability and Genetic Advance
Estimates of heritability in the broad sense (H2) and
genetic advance as percent of the mean (GAM) for 23
traits of potato genotypes are presented in Table 3. The
associated values for heritability in the broad sense and
genetic advance as percent of mean ranged from 38.13 to
91.64% and 1.28 to 73.50%, respectively. Heritability and
genetic advance as percent of mean values were high for
plant height, average stem number, leaf area index, shoot
dry mass weight, average tuber number, average tuber
weight, total tuber yield, marketable tuber yield,
unmarketable tuber yield, small size tubers, large size
tubers and length to width ratio. A similar study carried by
Ikbal and Khan (2003); Ummyiah et al. (2010); Getachew
et al. (2016); Tripura et al. (2016) reported for plant height,
average stem number, average tuber number, average
tuber weight, total tuber yield and marketable tuber yield
both high heritability (> 60%) and genetic advance (> 20%)
as a percent of the mean.
High heritability values associated with moderate values of
genetic advance as percent of mean were recorded for
days to 50% flowering, days to maturity, sphericity of the
tuber, dry matter content and total starch content.
Heritability and genetic advance as percent of mean
values moderate for tubers dry mass weight, total biomass
weight, medium-size tubers, and geometric mean
diameter. Ummyiah et al. (2010) reported high heritability
(>60%) with moderate genetic advance (>20%) as a
percent of the mean for dry matter content. Addisu et al.
(2013); Getachew et al. (2016) reported moderate
heritability (30-60%) associated with moderate genetic
advance as percent of the mean (10-20%) for total
biomass weight and medium-size tuber.
CONCLUSION
The difference between the values of PCV and GCV was
low (<5%) for the majority of the traits. This suggested that
most of the traits were less influenced by environmental
factors and selection based on phenotype expression of
the genotypes could be applied as breeding methods to
improve the traits. The use of heritability and genetic
advance is used to determining the degree of genetic gain
from the selection of a trait. The selection efficiency for
yield and processing quality can be obtained by identifying
traits that exhibit high GA and heritability. The variation
within the traits means that there is a possibility of
maximizing gains during crop improvement. Average tuber
number, average tuber weight, specific gravity of tubers,
dry matter content and total starch content, are major traits
used during selection for yield and processing quality.
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Genetic Variability, Heritability and Genetic Advance in Potato (Solanum Tuberosum L.) for Processing Quality, Yield and Yield Related Traits
Int. J. Plant Breed Crop Sci. 936
Accepted 6 November 2020
Citation: Ebrahim S, Wassu M, Tessfaye A (2020). Genetic Variability, Heritability and Genetic Advance in Potato
(Solanum Tuberosum L.) for Processing Quality, Yield and Yield Related Traits. International Journal of Plant Breeding
and Crop Science, 7(3): 928-936.
Copyright: © 2020: Ebrahim et al. This is an open-access article distributed under the terms of the Creative Commons
Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original
author and source are cited.

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Genetic Variability and Advance in Potato for Quality and Yield

  • 1. Genetic Variability, Heritability and Genetic Advance in Potato (Solanum Tuberosum L.) for Processing Quality, Yield and Yield Related Traits Genetic Variability, Heritability and Genetic Advance in Potato (Solanum Tuberosum L.) for Processing Quality, Yield and Yield Related Traits Ebrahim Seid1*, Wassu Mohammed2 and Tessfaye Abebe1 1Ethiopia Institute of Agricultural Research, Holetta Agricultural Research Centre, P. O. Box: 2003, Addis Ababa, Ethiopia. 2Haramaya University, P.O.Box 138, Dire Dawa, Ethiopia. Potato (Solanum tuberosum L.) used as fresh products and commercially processed foods such as French fries and chips.The objective of the experiment was to assess the nature and magnitude of variability in potato genotypes for tuber quality, yield and yield-related traits. Twenty four potato genotypes were evaluated at Holetta Agricultural Research Centre using a randomized complete block design with three replications during the growing season of 2017. The results of the analysis of variance indicated there was highly significant differences among the genotypes for all traits excepted peel content. The phenotypic (PCV) and genotypic (GCV) coefficient of variation ranged between 0.90 to 46.43% and 0.75 to 40.0%. Heritability in the broad sense (H2 ) and genetic advance as percent of the mean (GAM) ranged from 38.13 to 91.64% and 1.28 to 73.50%. High phenotypic coefficients of variation and genotypic coefficients of variation coupled with high heritability and genetic advance as percent of mean were observed for shoot dry mass weight, average tuber number, average tuber weight, unmarketable tuber yield, small size tuber and large size tubers. Therefore, selection for these characters would be effective for the emerging processing industry and could be selected as parents for future crossing program in Ethiopia. Keywords: Tuber quality, potato variability, genotypic variation, phenotypic variation, co-efficient of variance INTRODUCTION Potato (Solanum tuberosum L.) is the third most important food crop in the world after rice and wheat in terms of human consumption. More than a billion people eat potato worldwide, and global total production exceeds 300 million metric tons. Ther are more than 4,000 varieties of native potato and also over 180 wild species, mostly found in the Andes (CIP, 2020). In central highlands Ethiopia an adaptation trial of potato was conducted to identify potato varieties that is better to adaptation, yield and other agronomic traits and disease tolerant. The national average potato yield in Ethiopia is 13.9 t ha-1 (CSA., 2018), which is lower than the experimental yield of over 35 t ha - 1 (Berihun, 2013), and world average yield to 20 t ha-1 (FAOSTAT., 2019). It is a nutritious vegetable containing 16% carbohydrates, 2% proteins, 1% minerals, 0.6% dietary fiber and a negligible amount of fat (Gumul et al., 2011). In Eastern African potato is the high yield potential and plasticity to environmental regimes makes it on of the best crops for food and nutrition security (Kyamanywa et al., 2011). The large proportion (60 to 80%) of dry matter is composed of starches from 20% of the average dry matter content, it is a food rich in carbohydrate (Lutaladio and Castaidi, 2009). Besides being a rich source of carbohydrates, potato also contains some health- promoting compounds such as phenolic acids, ascorbic acid and carotenoids (Ezekiel et al., 2013). *Corresponding Author: Ebrahim Seid; Ethiopia Institute of Agricultural Research, Holetta Agricultural Research Centre, P. O. Box: 2003, Addis Ababa, Ethiopia *1 Corresponding Author Email: ibrahussen32@gmail.com 2 Co-Author Email: wasmoha@yahoo.com 1 Co-author Email: destaadera@gmail.com International Journal of Plant Breeding and Crop Science Vol. 7(3), pp. 928-936, November, 2020. © www.premierpublishers.org, ISSN: 2167-0449 Research Article
  • 2. Genetic Variability, Heritability and Genetic Advance in Potato (Solanum Tuberosum L.) for Processing Quality, Yield and Yield Related Traits Ebrahim et al. 929 The genetic makeup, crop maturity, agronomic practices, environmental conditions, storage temperatures, pests and diseases are affecting tuber. Studying the genetic variability present among different potato genotypes for a given character is a basic precondition to design systematic breeding methods. Predominantly, genetic variability for a given character is a basic precondition for its improvement by systematic breeding (Engida et al., 2007; Arslanoglu et al., 2011). Strategic potato research in Ethiopia began in 1975 with the understanding of the constraints challenging its production and productivity (Baye and Gebremedhin, 2013). The development and dissemination of more than 36 ( Belete, Gudanie, Dagim and Jalene…) improved varieties, coupled with other technological packages, contributed greatly to the improvement and rapid expansion in potato production (MANR, 2016). The major objective of potato breeding has been to develop potato cultivars that have maximum yield potential, adaptable to wide agro-ecologies and resistant to late blight that has been the most devastating disease throughout the dominant potato producing highlands of the country (Wassu, 2016). Few studies have been conducted on processing quality potato genotypes (Elfnesh et al., 2011; Tesfaye et al., 2012; Wassu, 2016; Wassu, 2017). However, the released varieties have not satisfied the consumer for French fries and chips making process (Habtamu et al., 2016). Therefore, it is necessary to conduct a comprehensive study on genetic variability of available genotype for tuber quality, yield and yield-related traits and document information on the available varieties quality features. The present experiment was conducted to assess the nature and magnitude of variability in potato genotypes for tuber quality, yield and yield-related traits with the help of genetic parameters, such as phenotypic as well as genotypic coefficient of variation and genotypic coefficients of variation and estimate of heritability in the broad sense. MATERIALS AND METHODS Experimental Site, Design and Materials A total of 24 potato genotypes were used for the experiment. These included 21 genotypes and three released varieties (Table 1). The 24 genotypes were planted at Holleta Agricultural Research Centre experiment station during the main cropping season of 2017. The experiment was laid out in randomized complete block design (RCBD) with three replications and each plot was 3.6 m (length) x 4.5 m (width) (16.2 m2 gross plot size) consisted six rows each containing 12 plants and thus 72 plants per plot. The spacing between rows and plants was 0.75 m and 0.30 m, respectively. The spacing between plots and adjacent replications was 1 m and 1.5 m, respectively. Table 1: List of experimental materials included in the study No. Accession code No. Accession code 1 CIP-396034.268 13 CIP-394611.112 2 CIP-393220.54 14 CIP-392617.54 3 CIP-395017.229 15 CIP-381381.20 4 CIP-392797.27 16 CIP-398180.289 5 CIP-395112.19 17 CIP-.398190.89 6 CIP-399075.7 18 CIP-398190.404 7 CIP-393280.64 19 CIP-391058.175 8 CIP-398098.65 20 CIP-396034.103 9 CIP-393385.39 21 CIP-391046.14 10 CIP-396027.205 22 Belete (CIP-393371.58) 11 CIP-393077.159 23 Gudanie (CIP-386423.13) 12 CIP-399002.52 24 Dagim (CIP-396004.337) CIP = International Potato Center Data Collection Phenology and Growth Parameters Data was recorded for phenology and growth parameters; days to 50% flowering, days to maturity, plant height (cm), average stems number and leaf area index (cm-3). Yield and Yield Components Data was recorded for yield parameters; shoot dry mass weight (g), tubers dry mass weight (g): total biomass weight (g), average tuber number /hill, average tuber weight (g/tuber), tuber size distribution:- small (< 35mm), medium (35 to 50mm), and large (>50 mm) size tubers (%), total tuber yield (t/ha), marketable tuber yield(/ha) and unmarketable tuber yield(t/ha). The amount of tuber number in different size categories was changed to a percentage (Ekin et al., 2009). Tuber Physical and Internal Quality Traits Peel content (%): Ten fresh tubers were randomly selected from each plot, weight and peeled. The peel of the tubers was weighted. The mean weight of single tuber and peel content was recorded and then expressed as a percentage as follows: Peel content (%) = tuberofWeight contentpeel x 100 Geometric mean diameter (Dg) (mm): The sizes of ten randomly selected tubers from each plot were measured as length, width and thickness using a digital caliper with an accuracy of 0.01mm. The following equation calculated the geometric mean diameter (Dg): Dg= (LWT) 0.333 where: L is length; W is width and T is the thickness of the tuber.
  • 3. Genetic Variability, Heritability and Genetic Advance in Potato (Solanum Tuberosum L.) for Processing Quality, Yield and Yield Related Traits Int. J. Plant Breed Crop Sci. 930 Length to width ratio: Recorded as the ratio of tubers length to width and then expressed in terms ratio. Sphericity of tuber () (%): the sphericity was determined by the following formula as described by Ahmadi et al. (2008):  = (Dg/L) x 100 Where,  is the sphercity of the tuber, Dg is the geometric mean diameter and L is the length Surface area (S) (mm2 ): Tubers surface area was determined according to Bareh (2000) by the following formula: S =  Dg2 where: S is surface area and Dg is the geometric mean diameter Specific gravity of tubers (Sg) (gcm-3 ): It was determined using the weight in air/weight in water methods. Five kilogram tubers of all shapes and sizes were randomly taken from each plot. The selected tubers were washed with water. First, it was weight in air and then re-weighed suspended in water and the specific gravity was determined according to the following formula (Gould, 1995). Specific gravity = weight in air weight in air − weight in water Dry matter content (%): The total dry matter content (DMC) was calculated according to Porras et al. (2014). Five tubers of each plot were chopped (about 500g total) into small 1-2 cm cubes. They were mixed thoroughly and two subsamples of 200g each were taken. The exact weight of each subsample was recorded as fresh weight. Consequently, each subsample was placed in an oven set at 80°C for 48 hours and dried until constant weight. Each subsample were weighted immediately and recorded as dry weight. The dry matter content for each subsample was then computed with the following formula. Dry matter content (%) = dry weight fresh weight ∗ 100 Total starch content (g/100g): This was estimated from dry matter content. Total starch content (g/100g): This was estimated from dry matter. Starch content (%) = 17.55 + 0.891* ( tuber dry weight % - 24.182) AOAC, 1980) where the dry matter was determined as indicated above it was measured from tubers of the five randomly selected plants to be used for tuber dry mass estimation was sliced and kept in an oven at 80°C for 48 hours and weighed after cooling in room temperature. Data Analysis Analysis of variance The data was subjected to analysis of variance (ANOVA) using the SAS statistical software 9.2 (SAS, 2008). The comparison of the mean performance of genotypes was done the significance of mean squares using Duncan’s multiple range test (DMRT). The traits that exhibited significant mean squares in general ANOVA was further subjected to genetic analyses. Phenotypic and Genotypic Variability The genotypic and phenotypic variability of each quantitative traits was estimated as genotypic and phenotypic variances and coefficients of variation. The genotypic and phenotypic coefficient of variation was determined by the formula as described by Burton and de Vane (1953) Genotypic variance (σ2g) = 𝑀𝑆𝑔−𝑀𝑆𝑒 𝑟 Where, σ2g = genotypic variance, MSg = mean square of genotype, MSe = mean square of error, r = number of replications and environmental variance (𝜎2 𝑒) = mean square of the error Phenotypic variance (σ2p) = σ2g + σ2e Where, σ2g = genotypic variance, σ2e = Environmental variance and σ2p = Phenotypic variance 100 x _ 2 p PCV              And 100_ 2             x g GCV  Where: PCV= Phenotypic coefficient of variation, GCV= Genotypic coefficient of variation and 𝐱 = population mean of the character being evaluated PCV and GCV values were categorized as low, moderate, and high values, as indicated by Sivasubramaniah and Menon (1973) as follows. 0-10% = low, 10-20% moderate and also > 20% = High Heritability and Genetic Advance Broad sense heritability values were estimated using the formula adopted by Falconer and Mackay (1996) as follows: H2 = (σ2g/σ2p) x 100 Where, H2 = heritability in broad sense, σ2p = Phenotypic variance and σ2g = genotypic variance The heritability percentage was categorized as low, moderate and high, as suggested by Robinson and Callbeck (1955). 0-30% = low, 30-60% = moderate and > 60% = high Expected genetic advance under selection (GA): Expected genetic advance (GA) at 5% percent selection intensity and genetic advance as percentage of mean (GAM) was calculated through the method of Johnson et al. (1955) GA = K*SDp*H2 Where, GA = Genetic advance, SDp = phenotypic standard deviation on mean basis, H2 = Heritability in the broad sense and K = standardized selection differential at 5% selection intensity (K = 2.063)
  • 4. Genetic Variability, Heritability and Genetic Advance in Potato (Solanum Tuberosum L.) for Processing Quality, Yield and Yield Related Traits Ebrahim et al. 931 Genetic advance as percent of the mean (GAM) Genetic advance as percent of mean was estimated as follows: GAM = GA X X100 Where GAM = Genetic advance as percent of the mean, GA = genetic advance and X = Population mean of the character being evaluated The magnitude of genetic advance as percentage of mean was categorized as low (0-10%), moderate (10- 20%), and high (> 20%), as suggested by Johanson et al. (1955). RESULT AND DISCUSSION Analysis of Variance The results of the analysis of variance indicated the presence of highly significant (p<0.01) differences among the tested potato genotypes for all traits excepted peel content (Table 2). This will provide a good opportunity for the breeder to select genotypes of varied maturity groups, tuber yield performance and traits related to processing aspects. Several workers, Misgana et al. (2015); Getachew et al. (2016); Habtamu et al. (2016); Wassu (2016); Wassu (2017) reported highly significant differences among genotypes, for phenology, tuber yield and processing quality traits. Table 2: Mean squares from analysis of growth parameters yield and quality-related traits and yield of 24 potato genotypes evaluated at Holetta in 2017. Traits Mean Replication (2) Genotype (23) Error (46) CV (%) Days to 50% flowering 54.19 7.51 54.72** 1.82 2.49 Days to maturity 95.92 18.38 117.86** 13.04 3.77 Plant height (cm) 80.48 385.17 495.62** 14.62 4.75 Average stems number 4.05 0.67 1.86** 0.24 12.06 Leaf area index 2.56 0.17 0.55** 0.05 8.95 Shoot dry mass weight (g/plant) 46.00 145.82 617.52** 69.66 18.14 Tuber dry mass weight (g/plant) 166.14 497.39 1918.32** 558.03 14.22 Total biomass weight (g/plant) 212.14 593.79 2790.60** 723.03 12.68 Average tuber number per hill 11.08 12.49 16.43** 1.58 11.34 Average tuber weight (g/tuber) 66.36 15.19 640.17** 34.77 8.89 Small size tubers (%) 34.12 259.42 248.00** 41.86 18.96 Medium size tubers (%) 39.93 143.46 105.25** 30.34 13.79 Large size tubers (%) 25.95 30.79 351.23** 28.03 20.41 Total tuber yield (t/ha) 31.63 59.35 91.85** 11.70 10.81 Marketable tuber yield (t/ha) 28.74 42.02 75.09** 11.65 11.88 Unmarkatable tuber yield (t/ha) 2.89 1.63 4.37** 0.52 24.82 Peel content (%) 14.85 5.73 4.73ns 3.41 12.39 Geometric mean diameter (mm3) 55.60 13.19 37.24** 13.07 6.50 Sphericity of the tuber (%) 80.20 0.01 179.79** 7.20 3.35 Surface area (mm2) 9772.71 1626891.6 4480789.00** 1552507.4 12.75 Length to width ratio 1.27 0.002 0.122** 0.005 5.64 Specific gravity of tubers (g/cm3) 1.09 0.00000768 0.00022599** 0.00002919 0.50 Dry matter content (%) 21.94 0.34 10.83** 1.39 5.37 Total starch content (g/100g) 15.55 0.26 8.60** 1.10 6.76 ** - Significant at P<0.01 and ns - non-significant, number in parenthesis represented degree of freedom for the respective source of variation, cm = centimeter, g= gram, t/ha= ton per hectare, mm3 = millimeter cub, mm2 = millimeter square and g/cm3 = gram per centimeter cub
  • 5. Genetic Variability, Heritability and Genetic Advance in Potato (Solanum Tuberosum L.) for Processing Quality, Yield and Yield Related Traits Estimates of Variability Components Phenotypic and Genotypic Variation The phenotypic coefficient of variation (PCV) and genotypic coefficient of variation (GCV) ranged from 0.90 to 46.43% and 0.75 to 40.0%, respectively (Table 3). Sivasubramaniah and Menon (1973) categorized PCV and GCV coefficient of variation values low (<10%), moderate (10-20), and high (>20%). Based on these categories, days to 50% flowering, days to maturity, geometric mean diameter, sphericity of the tuber, specific gravity of tuber and dry matter content showed low PCV and GCV except sphericity the tuber that had moderate PCV. Plant height, average stems number, leaf area index, tubers dry mass weight, total biomass weight, total tuber yield, marketable tuber yield, medium size tuber, surface area, length to width ratio and total starch content had both moderate PCV and GCV values except average stems number that had high PCV values. Shoot dry mass weight, average tuber number, average tuber weight, unmarketable tuber yield, small size tubers and large size tuber traits had high PCV and GCV values. The high PCV and GCV indicated that the traits are controlled by genetic factors and hence there is a higher chance of improvement of the crop through selection. Singh (1990) suggested that traits that exhibited high estimates of GCV and PCV have a high probability of improvement through selection. However, the author indicated the improvement of traits is difficult and impractical through selection for traits that exhibited low estimates for both variability components due to the masking effect of the environment on the genotypic effect. Int. J. Plant Breed Crop Sci. 932 A similar study carried by Regassa and Basavaraj (2005); Addisu et al. (2013); Getachew et al. (2016); Tripura et al. (2016); Panigrahi et al. (2017) observed high GCV and PCV for average tuber number, average tuber weight, small size tubers, large size tubers and unmarketable tuber yield. Ummyiah et al. (2010) reported moderate PCV and GCV for total tuber yield and low PCV and GCV for specific gravity and dry matter content. Ara et al. (2009), Mishra et al. (2017) reported moderate PCV and GCV for total starch content, shoot dry mass weight and average stems number.
  • 6. Genetic Variability, Heritability and Genetic Advance in Potato (Solanum Tuberosum L.) for Processing Quality, Yield and Yield Related Traits Ebrahim et al. 933 Table 3: Estimate of variability components for 23 traits in 24 potato genotypes evaluated at Holetta in 2017 σ2g σ2p σ2e PCV (%) GCV (%) H2 (%) GA GAM (5 %) Difference between PCV & GCV Days to 50% flowering 17.63 19.45 1.82 8.14 7.75 90.65 8.25 15.22 0.39 Days to maturity 34.94 47.98 13.04 7.22 6.16 72.82 10.41 10.85 1.06 Plant height (cm) 160.33 174.95 14.62 16.44 15.73 91.64 25.01 31.07 0.71 Average stems number 0.54 0.78 0.24 21.81 18.17 69.43 1.27 31.24 3.64 Leaf area index 0.17 0.22 0.05 18.30 15.97 76.10 0.74 28.73 2.33 Shoot dry mass weight (g/plant) 182.62 252.28 69.66 34.53 29.38 72.39 23.72 51.56 5.15 Tubers dry mass weight (g/plant) 453.43 1011.46 558.03 19.14 12.82 44.83 29.41 17.70 6.32 Total biomass weight (g/plant) 689.19 1412.23 723.04 17.71 12.38 48.80 37.83 17.83 5.33 Average tuber number per hill 4.95 6.53 1.58 23.06 20.08 75.80 4.00 36.06 2.98 Average tuber weight (g/tuber) 201.80 236.57 34.77 23.18 21.41 85.30 27.07 40.79 1.77 Small size tubers (%) 68.71 110.57 41.86 30.82 24.29 62.14 13.48 39.51 6.53 Medium size tubers (%) 24.97 55.31 30.34 18.62 12.51 45.15 6.93 17.35 6.11 Large size tubers (%) 107.73 135.77 28.03 44.90 40.00 79.35 19.07 73.50 4.90 Total tuber yield (t/ha) 26.72 38.42 11.70 19.60 16.34 69.54 8.89 28.12 3.26 Marketable tuber yield (t/ha) 21.14 32.80 11.65 19.93 16.00 64.47 7.62 26.50 3.93 Unmarketable tuber yield (t/ha) 1.29 1.80 0.52 46.43 39.23 71.41 1.98 68.39 7.20 Geometric mean diameter (mm3) 8.06 21.13 13.07 8.27 5.10 38.13 3.62 6.50 3.17 Sphericity of the tuber (%) 57.53 64.73 7.20 10.03 9.46 88.87 14.75 18.39 0.57 Surface area (mm2) 976093.87 2528601.27 1552507.40 16.27 10.11 38.60 1266.34 12.96 6.16 Length to width ratio 0.04 0.04 0.01 16.52 15.52 82.32 0.38 30.10 1.00 Specific gravity of tubers (g/cm3) 0.0000656 0.0000948 0.00002919 0.90 0.75 69.21 0.01 1.28 0.15 Dry matter content (%) 3.15 4.54 1.39 9.71 8.09 69.40 3.05 13.90 1.62 Total starch content (g/100g) 2.50 3.60 1.10 12.20 10.16 69.35 2.72 17.46 2.04 σ2g = genotypic variance, σ2p = phenotypic variance. σ2e = environment variance, PCV = phenotypic coefficient of variation, GCV = genotypic coefficient, H2 = heritability in broad sense, GA = genetic advance, GAM = genetic advance as percent of mean, cm = centimeter, g= gram, t/ha= ton per hectare, mm3 = millimeter cub, mm2 = millimeter square and g/cm3 = gram per centimeter cub
  • 7. Genetic Variability, Heritability and Genetic Advance in Potato (Solanum Tuberosum L.) for Processing Quality, Yield and Yield Related Traits Int. J. Plant Breed Crop Sci. 934 Estimate of Heritability and Genetic Advance Estimates of heritability in the broad sense (H2) and genetic advance as percent of the mean (GAM) for 23 traits of potato genotypes are presented in Table 3. The associated values for heritability in the broad sense and genetic advance as percent of mean ranged from 38.13 to 91.64% and 1.28 to 73.50%, respectively. Heritability and genetic advance as percent of mean values were high for plant height, average stem number, leaf area index, shoot dry mass weight, average tuber number, average tuber weight, total tuber yield, marketable tuber yield, unmarketable tuber yield, small size tubers, large size tubers and length to width ratio. A similar study carried by Ikbal and Khan (2003); Ummyiah et al. (2010); Getachew et al. (2016); Tripura et al. (2016) reported for plant height, average stem number, average tuber number, average tuber weight, total tuber yield and marketable tuber yield both high heritability (> 60%) and genetic advance (> 20%) as a percent of the mean. High heritability values associated with moderate values of genetic advance as percent of mean were recorded for days to 50% flowering, days to maturity, sphericity of the tuber, dry matter content and total starch content. Heritability and genetic advance as percent of mean values moderate for tubers dry mass weight, total biomass weight, medium-size tubers, and geometric mean diameter. Ummyiah et al. (2010) reported high heritability (>60%) with moderate genetic advance (>20%) as a percent of the mean for dry matter content. Addisu et al. (2013); Getachew et al. (2016) reported moderate heritability (30-60%) associated with moderate genetic advance as percent of the mean (10-20%) for total biomass weight and medium-size tuber. CONCLUSION The difference between the values of PCV and GCV was low (<5%) for the majority of the traits. This suggested that most of the traits were less influenced by environmental factors and selection based on phenotype expression of the genotypes could be applied as breeding methods to improve the traits. The use of heritability and genetic advance is used to determining the degree of genetic gain from the selection of a trait. The selection efficiency for yield and processing quality can be obtained by identifying traits that exhibit high GA and heritability. The variation within the traits means that there is a possibility of maximizing gains during crop improvement. Average tuber number, average tuber weight, specific gravity of tubers, dry matter content and total starch content, are major traits used during selection for yield and processing quality. REFERENCES Addisu F, Yohannes P, Habtamu Z. (2013). 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  • 9. Genetic Variability, Heritability and Genetic Advance in Potato (Solanum Tuberosum L.) for Processing Quality, Yield and Yield Related Traits Int. J. Plant Breed Crop Sci. 936 Accepted 6 November 2020 Citation: Ebrahim S, Wassu M, Tessfaye A (2020). Genetic Variability, Heritability and Genetic Advance in Potato (Solanum Tuberosum L.) for Processing Quality, Yield and Yield Related Traits. International Journal of Plant Breeding and Crop Science, 7(3): 928-936. Copyright: © 2020: Ebrahim et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are cited.