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Design PCR primers template

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This document discusses the design of PCR primers for a DNA template. A forward primer and reverse primer were designed that are complementary to the 5' and 3' ends of the template respectively. The forward primer is 5'-AGACTCAGAGAGAACCC-3' and the reverse primer is 5'-ATTCAAAGACCACGGGG-3'.

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1 Design of PCR primers Template 3’-TCTGAGTCTCTCTTGGG------GGGGCACCAGAAACTTA-5’ 5’-AGACTCAGAGAGAACCC------CCCCGTGGTCTTTGAAT-3’ Forward primer – 5’-AGACTCAGAGAGAACCC-3’ Reverse primer – 5’-ATTCAAAGACCACGGGG-3’

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Design PCR primers template

  • 1. 1 Design of PCR primers Template 3’-TCTGAGTCTCTCTTGGG------GGGGCACCAGAAACTTA-5’ 5’-AGACTCAGAGAGAACCC------CCCCGTGGTCTTTGAAT-3’ Forward primer – 5’-AGACTCAGAGAGAACCC-3’ Reverse primer – 5’-ATTCAAAGACCACGGGG-3’