Why are culture media sterilized before they are used? Do you think that slants are autoclaved before or after they are solidified? Explain. Solution Answer:-4:-The culture media are sterlized for preventing the explant from being infected by bacteria, spores or fungal infectuon. Its done through milipore filter because autoclaving would decompose the organic nutrients (Amino acids, vitamins, glucose) as they are thermo labile. Answer:-4:-Slabts are autoclaved before beacuse after autoclaving it would melt agar nutrient medium..