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Why is it important to reduce disulfide linkages prior to SDS electrophoresis?
A. to help maintain the globular structure of the protein
B. to maximize the number of positive charges on the protein
C. to allow the protein to assume a completly linear form
D. to allow the -SH groups to interact with the gel material
E. to maximaize the number of negative charges on the protein
What is NOT true about protein migration during polyacrylamide gel electrophoresis?
A. movement depends on protein size
B. positvely charged proteins move toward the anode (+)
C. movement depends on protein shape
D. smaller proteins mvoe faster than larger proteins
E. movement depends on protein charge
What is true about NAD+/NADH
a. can only act as a reducing agent
b. does not contain a vitamin component
c. can only act as an oxidizing agent
d. contains ribose, phosphate, and adenine
What is NOT true about FAD/FADH?
a. its oxidation-reduction reactions involve the transfer of two electrons and two protons
b. its phosphates are directly involved in energy transfer
c. contains a vitamin component
d. contains two phosphates
FMNH and FADH differ in?
a. the adenine component
b. their vitamin component
c. the ribitol component
d. the number of electrons and protons they transfer

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  • 1. Why is it important to reduce disulfide linkages prior to SDS electrophoresis? A. to help maintain the globular structure of the protein B. to maximize the number of positive charges on the protein C. to allow the protein to assume a completly linear form D. to allow the -SH groups to interact with the gel material E. to maximaize the number of negative charges on the protein What is NOT true about protein migration during polyacrylamide gel electrophoresis? A. movement depends on protein size B. positvely charged proteins move toward the anode (+) C. movement depends on protein shape D. smaller proteins mvoe faster than larger proteins E. movement depends on protein charge What is true about NAD+/NADH a. can only act as a reducing agent b. does not contain a vitamin component c. can only act as an oxidizing agent d. contains ribose, phosphate, and adenine What is NOT true about FAD/FADH? a. its oxidation-reduction reactions involve the transfer of two electrons and two protons b. its phosphates are directly involved in energy transfer c. contains a vitamin component d. contains two phosphates FMNH and FADH differ in?
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