BSC NURSING NOTE

2. Introduction
 Medically important Pseudomonas:
P. aeruginosa
 Commonly present in moist environments in
hospitals.
 It is primarily a nosocomial pathogen.

3.  Gram-negative rods.
 Motile by polar flagella.
 aerobics.
 Grow well at 42⁰C.
 Non-lactose fermenting

4. Pathogenesis

6.  can produce pigments, such as:
 Pyocyanine (blue-green)
 Pyoverdin (fluorescent yellow- greenish
pigment)
 Pyorubrin (red)
 Pyomelanin (brown)

7. Specimen:
 Wound discharge
 sputum
 Blood
 Urine
 CSF

8. Gram stain
 Gram negative rod
undistinguished from
enerobacteriaceae

10.  Form smooth and round colonies.
 Fluorescent greenish colour.
 production of fruity odor (grape-like) .
 Inability to ferment lactose.

11.  Colonies are smooth, large, translucent
 Greenish blue diffusible pigment

12.  Grayish colonies
 Many are haemolytic
(beta hemolysis).
This picture shows Pseudomonas
aeruginosa (on the right) and Shigella
dysenteriae (on the left) in blood agar.

13.  Non-lactose fermenting (colourless colonies)

14. Pseudomonas aeruginosa fluorescence under UV
illumination

16. Oxidase test
 Oxidase positive
Catalase test
 Catalase positive