Using an inoculating loop, demonstrate how to aseptically remove some inoculum from either a broth tube, slant tube, or petri plate and inoculate a sterile broth tube, slant tube or petri plate without introducing outside contamination. Solution Answer: 1) Before inserting loop into bacterial culture (broth tube, slant tube and petriplate), loop should be kept in flame until it appears red, after that wait for few seconds before picking a bacterial culture (as loop with more heat may kill bacterial culture) and touch from one end culture and transfer into to new nutrient medium. All this procedure should be done in sterile cabin called as laminar air flow which blows the air continuosly..

1. Using an inoculating loop, demonstrate how to aseptically remove some inoculum from either a
broth tube, slant tube, or petri plate and inoculate a sterile broth tube, slant tube or petri plate
without introducing outside contamination.
Solution
Answer:
1) Before inserting loop into bacterial culture (broth tube, slant tube and petriplate), loop should
be kept in flame until it appears red, after that wait for few seconds before picking a bacterial
culture (as loop with more heat may kill bacterial culture) and touch from one end culture and
transfer into to new nutrient medium.
All this procedure should be done in sterile cabin called as laminar air flow which blows the air
continuosly.