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As its name implies, connective tissue connects,
holds, and supports other body tissues.
The cells of connective tissue are characteristically
separated from one another by large amounts of
intercellular material.
This extracellular material is that gives connective
tissue its strenght.
Parts of the connective tissue:
Cells
Extracellular matrix (ECM)
Two types of cells are present in the
            connective tissue
FIXED                    MOBILE

Fibroblast (fibrocyte)   Plasma cell
Fat cells (adipocyte)    Mast cell
Melanocyte               Histiocyte
Mesoblast                (macrophage)
Reticular cell           Lymphocyte
                         Granulocyte
Fibers             Amorphous material

Collagen fibers    GAG and
Elastic fibers     proteoglycans
Reticular fibers   Adhesion molecules
Microfibrils
Mesenchyma
Embryonic
            Wharton s jelly
                                Areolar
             Rich in cells      Reticular
                              Spinocellular
 Mature
                                 Loose
            Rich in fibers                    Regular
                                 Dense
                                              Irregular
Slide 4, Unilocular fat (paraffin section, HE), 10X




                                adipocytes




                         vessels
Slide 4, Unilocular fat (paraffin section, HE), 40X




         adipocytes



                                                      vessels




               Nuclei of adipocytes
To identify the following structures:

  adipocytes and their nuclei
  vessels
Slide 5, Unilocular fat (frozen section, Sudan), 4X




   epidermis           dermis               hypodermis
Slide 5, Unilocular fat (frozen section, Sudan), 10X



                                        adipocytes




            panniculus adiposus
Slide 5, Unilocular fat (frozen section, Sudan), 40X




                          adipocytes
To divide the 3 parts of the skin
To identify the adipocytes
Slide 78, Skin (HE), 4X




    epidermis




    dermis




    hypodermis
Slide 78, Skin (HE), 10X




irregular dense connective tissue loose connective tissue

              dermis                    hypodermis
To divide the 3 parts of the skin
To identify the:
loose connective tissue
dense connective tissue
Slide 6, Tendon (HE), 10X




              regular dense connective tissue
Slide 6, Tendon (HE), 40X




                            fibrocytes




               collagen bundles
To identify:
collagen bundles
fibrocytes
Cartilage consists of cells (chondrocytes)
   and an extensive extracellular matrix
   composed of fibers and ground substance.
   3 forms of cartilage have evolved, each
   exhibiting variations in matrix composition:
1. Hyaline cartilage
2. Elastic cartilage
3. Fibrocartilage
1. Hyaline cartilage: in the articular surfaces of the
   movable joints; the walls of larger respiratory
   passages (nose, larynx, trachea, bronchi); the
   ventral ends of ribs; and in the epyphyseal
   plates of bones
2. Elastic cartilage: in the auricle of the ear; the
   walls of the external auditory canals, the
   auditory tubes, the epiglottis, and the cuneiform
   cartilage in the larynx
3. Fibrocartilage: in the intervertebral disks; and in
   articular meniscs
Perichondrium: outer layer (stratum fibrosum)
   type I collagen, inner layer (stratum
chondroblasticum)        rich of cells
The unit of cartilages: chondron
Chondrocyte: only one cell, or little groups of
cells, roundish shape, spherical nucleus
   In hyaline cartilage: 2-4 chondrocytes
   In elastic cartilage: 1-2 chondrocytes
   In fibrous cartilage: 1-2 chondrocytes
Lacuna
Pericellular matrix
Territorial/capsular matrix
Interterritorial matrix
Schematic representation of hyaline cartilage


                                             perichondrium




      chondron
                                           lacuna

                                           interterritorial
  chondrocyte                              matrix



pericellular matrix   territorial/capsular matrix
Slide 7, Hyaline cartilage (HE), 10X




                                                chondrons

              interterritorial matrix




                    stratum chondroblasticum
  perichondrium
                             stratum fibrosum
Slide 7, Hyaline cartilage (HE), 40X

                          lacuna

                             chondrocytes


 pericellular matrix


                                       interterritorial matrix
                          chondron
To identify the parts of hyaline cartilage:
perichondrium
chondron
chondrocyte
pericellular matrix
territorial matrix
interterritorial matrix
lacuna
Slide 8, ear (orcein), 10X



                                         perichondrium




                             chondrons



                 perichondrium
Slide 8, ear (orcein), 40X




                    chondrocytes

                                   elastic fibers



                                     chondron
To identify the components of the elastic
cartilage:
elastic fiber
chondron
chondrocyte
Slide 9, Articular menisc (HE), 40X




chondrocytes



                                      collagen fibers
To identify the parts of the fibrocartilage:
chondrocytes
collagen fibers
Schematic drawing of the bone

         outer circumferential lamellae
                                    Haversian system (osteon)

       compact bone
Inner circumferential
lamellae



   cancellous
   (spongy) bone
                                                       osteocyte
                                                        Haversian
                                                        canal
                                                      Volkmann s
                                                      canal
Slide 10, Bone (ground section) 10X

                   outer circumferential lamellae




    Volkmann s canal
                                          Haversian canal
                               osteon

             interstitial lamellae

                                                osteocytes



         inner circumferential lamellae
Slide 10, Bone (ground section) 40X




                       Haversian canal



   osteocytes


                                         osteon
To recognize the following structures:
osteon
outer and inner circumferential lamellae
interstitial lamellae
osteocyte
Volkmann s canal
Haversian canal
Enchondral
ossification
Slide 11, Enchondral ossification (HE) 4X




                                               bone marrow



                   bone spicules

                                      skeletal muscle




           enchondral ossification
Slide 11, Enchondral ossification (HE) 10X



                                                 resting zone




       bone marrow
                                         proliferative zone




                              hypertrofic cartilage zone

bone spicule              ossification zone
To identify:
enchondral ossification with its zones
bone marrow
bone spicules
skeletal muscle
adipose tissue

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Connective and supporting tissue

  • 1.
  • 2. As its name implies, connective tissue connects, holds, and supports other body tissues. The cells of connective tissue are characteristically separated from one another by large amounts of intercellular material. This extracellular material is that gives connective tissue its strenght. Parts of the connective tissue: Cells Extracellular matrix (ECM)
  • 3. Two types of cells are present in the connective tissue FIXED MOBILE Fibroblast (fibrocyte) Plasma cell Fat cells (adipocyte) Mast cell Melanocyte Histiocyte Mesoblast (macrophage) Reticular cell Lymphocyte Granulocyte
  • 4. Fibers Amorphous material Collagen fibers GAG and Elastic fibers proteoglycans Reticular fibers Adhesion molecules Microfibrils
  • 5. Mesenchyma Embryonic Wharton s jelly Areolar Rich in cells Reticular Spinocellular Mature Loose Rich in fibers Regular Dense Irregular
  • 6. Slide 4, Unilocular fat (paraffin section, HE), 10X adipocytes vessels
  • 7. Slide 4, Unilocular fat (paraffin section, HE), 40X adipocytes vessels Nuclei of adipocytes
  • 8. To identify the following structures: adipocytes and their nuclei vessels
  • 9. Slide 5, Unilocular fat (frozen section, Sudan), 4X epidermis dermis hypodermis
  • 10. Slide 5, Unilocular fat (frozen section, Sudan), 10X adipocytes panniculus adiposus
  • 11. Slide 5, Unilocular fat (frozen section, Sudan), 40X adipocytes
  • 12. To divide the 3 parts of the skin To identify the adipocytes
  • 13. Slide 78, Skin (HE), 4X epidermis dermis hypodermis
  • 14. Slide 78, Skin (HE), 10X irregular dense connective tissue loose connective tissue dermis hypodermis
  • 15. To divide the 3 parts of the skin To identify the: loose connective tissue dense connective tissue
  • 16. Slide 6, Tendon (HE), 10X regular dense connective tissue
  • 17. Slide 6, Tendon (HE), 40X fibrocytes collagen bundles
  • 19. Cartilage consists of cells (chondrocytes) and an extensive extracellular matrix composed of fibers and ground substance. 3 forms of cartilage have evolved, each exhibiting variations in matrix composition: 1. Hyaline cartilage 2. Elastic cartilage 3. Fibrocartilage
  • 20. 1. Hyaline cartilage: in the articular surfaces of the movable joints; the walls of larger respiratory passages (nose, larynx, trachea, bronchi); the ventral ends of ribs; and in the epyphyseal plates of bones 2. Elastic cartilage: in the auricle of the ear; the walls of the external auditory canals, the auditory tubes, the epiglottis, and the cuneiform cartilage in the larynx 3. Fibrocartilage: in the intervertebral disks; and in articular meniscs
  • 21. Perichondrium: outer layer (stratum fibrosum) type I collagen, inner layer (stratum chondroblasticum) rich of cells The unit of cartilages: chondron Chondrocyte: only one cell, or little groups of cells, roundish shape, spherical nucleus In hyaline cartilage: 2-4 chondrocytes In elastic cartilage: 1-2 chondrocytes In fibrous cartilage: 1-2 chondrocytes Lacuna Pericellular matrix Territorial/capsular matrix Interterritorial matrix
  • 22. Schematic representation of hyaline cartilage perichondrium chondron lacuna interterritorial chondrocyte matrix pericellular matrix territorial/capsular matrix
  • 23. Slide 7, Hyaline cartilage (HE), 10X chondrons interterritorial matrix stratum chondroblasticum perichondrium stratum fibrosum
  • 24. Slide 7, Hyaline cartilage (HE), 40X lacuna chondrocytes pericellular matrix interterritorial matrix chondron
  • 25. To identify the parts of hyaline cartilage: perichondrium chondron chondrocyte pericellular matrix territorial matrix interterritorial matrix lacuna
  • 26. Slide 8, ear (orcein), 10X perichondrium chondrons perichondrium
  • 27. Slide 8, ear (orcein), 40X chondrocytes elastic fibers chondron
  • 28. To identify the components of the elastic cartilage: elastic fiber chondron chondrocyte
  • 29. Slide 9, Articular menisc (HE), 40X chondrocytes collagen fibers
  • 30. To identify the parts of the fibrocartilage: chondrocytes collagen fibers
  • 31. Schematic drawing of the bone outer circumferential lamellae Haversian system (osteon) compact bone Inner circumferential lamellae cancellous (spongy) bone osteocyte Haversian canal Volkmann s canal
  • 32. Slide 10, Bone (ground section) 10X outer circumferential lamellae Volkmann s canal Haversian canal osteon interstitial lamellae osteocytes inner circumferential lamellae
  • 33. Slide 10, Bone (ground section) 40X Haversian canal osteocytes osteon
  • 34. To recognize the following structures: osteon outer and inner circumferential lamellae interstitial lamellae osteocyte Volkmann s canal Haversian canal
  • 36. Slide 11, Enchondral ossification (HE) 4X bone marrow bone spicules skeletal muscle enchondral ossification
  • 37. Slide 11, Enchondral ossification (HE) 10X resting zone bone marrow proliferative zone hypertrofic cartilage zone bone spicule ossification zone
  • 38. To identify: enchondral ossification with its zones bone marrow bone spicules skeletal muscle adipose tissue