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Reproduction in bacteria
- 1. Reproduction in Bacteria Sr. Kaslin Juliat SAL Assistant Professor St. Xavier’s College Mahuadanr Nilamber Pitamber University
- 2. Bacteria are the simplest, the smallest, and the most successful microorganisms. They were first discovered by Anton Leeuwenhoek (1676). In the five kingdom classification, they are placed in Kingdom Monera.
- 5. In Bacteria a small protuberance, called bud, develops at one end of the cell. Genome replication follows, and one copy of the genome gets into the bud. Then the bud enlarges, eventually become a daughter cell and finally gets separated from the parent cell. Budding
- 6. Mostly during unfavorable conditions, bacterial protoplasm undergoes compartmentalization and subsequent fragmentation, forming minute bodies called gonidia. Under favorable conditions, each gonidium grows to a new bacterium. It becomes apparent that prior to fragmentation the bacterial genome has to undergo repeated replication so that each fragment gets a copy of it. Fragmentation
- 7. It is the common type of reproduction under favorable conditions in which cell divides into two similar daughter cells. During the process, the bacterial chromosomes get attached to the cell membrane and replicates to the bacterial chromosomes. As the cell enlarges the daughter chromosomes gets separated. A cross wall is formed between the separating daughter chromosomes. It divides the cell into two daughter cells. The daughter cells soon grow to maturity within 20-30 minutes. Under favorable conditions many bacteria divide once in 20-30 minutes. Binary fission
- 9. Endospore are resting spores formed in some gram positive bacteria (Bacillus and Clostridium) during unfavourable conditions. They are formed within the cells. During this process a part of the protoplast becomes concentrated around the chromosome. A hard resistant wall is secreted around it. The rest of the bacterial cell degenerates; Endospore are very resistant to extreme physical conditions and chemicals. During favorable conditions the spore wall gets ruptured and the protoplasmic mass gives rise to a new bacterium. Endospore formation
- 11. Sexual reproduction occurs in the form of genetic recombination. There are three main methods of Genetic Recombination Sexual reproduction Sexual reproduction Transformation Transduction Conjugation
- 12. Conjugation •Bacteria showing conjugation are dimorphic, i.e., they have two types of cells, male (F+) or donor and female (F-) or recipient. •The male or donor cell possesses 1-4 sex pili on the surface and fertility factor (transfer factor, sex factor) in its plasmid. Fertility factor contains genes for producing sex pili and other characters needed for gene transfer. •Sex pili are 1- 4 narrow protoplasmic outgrowths. Both sex pili and fertility factor are absent in female or recipient cells.
- 13. If these two types of cells happen to come nearer, a piles of male cell establishes a protoplasmic bridge or conjugation tube with the female cell. It takes 6-8 minutes. Gene exchange can occur by two methods.
- 14. Transformation It is the absorption of DNA segment from the surrounding medium by a living bacterium. The phenomenon was discovered by Griffith in 1928. Its mechanism was worked out by Avery (1944). Receptivity for transformation is present for a brief period when the cells have reached the end period of active growth. At this time they develop specific receptor sites in the wall. Normally E. coli does not pick up foreign DNA but it can do so in the presence of calcium chloride.
- 15. Transduction •It is the transfer of foreign genes by means of viruses. •The process also occurs in E. coli and a number of other hosts. A virus may pick up gene of the host in place of its own gene during its multiplication in the host cell. •Such a virus is never virulent. It passes over the gene of the previous host to the new host. •Transducing viruses may carry the same genes (restricted transduction) or different genes (generalized transduction) at different times.
- 16. Thank You