MICROPROPAGATION OF Psidium guajava

1. Micropropagation of Psidium guajava L. (Guava)
Presented by Patel Sabera Banu
BT 14

2. • The growth and maintenance of plant cells ,tissues, organs or whole plants invitro in a sterile
environment on a nutrient medium.
ACCLIMATIZATION
ROOTING OF
SHOOTS
SHOOT
MULTIPLICATION
CULTURE
INITIATION AND
ESTABLISHMENT
SELECTION OF
EXPLANT

3. INTRODUCTION
• Common Name: Guava
• Also known as APPLE OF TROPICS”
• Botanical Name: Psidium guajava L.
• Family: Myrtaceae
• Origin: Mexico, Central and South America
• Growth form: Shrub or small tree
NEED OF TISSUE
CULTURE
Wilt diesease
Low yield
production
Propogation

4. MATERIAL AND METHODS
Plant Material:
Explant source:
Mature pot grown guava plant
Explant type:
Nodal segment- 2-2.5 cm
Surface Sterilization:
a)Tap water
b)5.2% sodium hypochlorite for 10 min
c)1% mercuric chloride for 5 min
d)Sterile distilled water

5. (A) stock guava plants in the greenhouse
(B) nodal section
(C) new shoots break out from healthy
nodal sections
(D) shoots proliferated
(E) elongated shoots
(F) rooted shoots by medium method
(G)rooted shoots by dipping method
(H)guava plantlets acclimatized into the
soil for 2 weeks
(I) guava plantlets acclimatized into the
soil for 10 weeks

6. Starting stage and
Antioxidant treatment
• Explant cultured on ½ MS medium
with 30 gm/l sucrose,1mg/LBAP and
6.5gm/l agar.
• Supplemented with antioxidants
(0.5%PVP mixture of 150mg/l citric
acid and 100mg/l ascorbic acid).
Multiplication stage
• Sprouted buds transferred to ½ MS
media or WPS media along with
sucrose, agar and medium with
varying BAP concentration(1,2 or 3
mg/l)
• Culture conditions: ph=5.8, autoclave
at 121℃ for 15mins, growth
chamber temp 25℃, photo period of
16Hrs.
Rooting stage
• Subcultured shoots (2-3cm) were
used.
• Single phase rooting: culturing of
shoots on ½ MS medium with
combination of
IBA+NAA.(2mg/leach)
• Two phase rooting: dipping shoot
base in 250mg/l solution of IBA or
NAA for 30 seconds then transfer
to ½ MS without growth regulators.

7. RESULT AND DISCUSSION
• STARTING STAGE
• MULTIPLICATION STAGE
• ROOTING STAGE

8. CONCLUSION
On the basis of the obtained results, it can be concluded that, media browning may be
avoided by adding citric and ascorbic acids in the starting medium.
The multiplication rate of guava was better on ½MS supplemented with 3 mg L BAP.
Enhanced rooting was obtained in medium supplemented with combination of both IBA
and NAA.