This document analyzes data from 85,637 email campaigns totaling 1.7 billion emails sent and 232 million emails opened to determine what factors influence open rates. Some of the key findings include that shorter subject lines under 30 characters performed best, personalization like adding a name did not reliably increase opens, and polite language like "please" and "thanks" as well as asking questions increased opens while all-caps and exclamation points decreased opens. The document advocates testing assumptions and focusing on compelling subject lines written in a natural voice to increase email open rates.