1. Maize primary root zone-specific plasma membrane proteome Zhe Zhang (Jenny) University of Missouri – Columbia Division of Biochemistry Bond Life Sciences Center
2. Differential elongation during water stress Elongation Cell division Differentiation What are plasma membrane-located proteins (e.g., transporters, aquaporins, receptors), that may function in water stress responses? Spollen WG, et al., BMC Plant Biol. 2008; 8: 32.
12. Zone-specific PM proteome? Elongation Cell division Differentiation Zone 4 (12-20 mm) Zone 3 (7-12 mm) Zone 2 (3-7 mm) Zone 1 (0-3 mm) Root tip Two biological replica were analyzed, and results combined for data analysis.
13. Zone-specific proteins (79/1028) 13 1 10 4 8 Zone 4 Zone 4 0 4 Zone 3 Zone 3 2 0 Zone 2 Zone 2 9 4 Zone 1 24 Zone 1 Root tip Root tip Total: 28 zone-specifically presentproteins Total: 29 zone-specifically absent proteins Total: 22 zone-specifically gradient proteins
18. Conclusion & perspectives Conclusion: PM-enrichment protocol worked well Background info on well-watered condition established Perspectives: WS vs WW comparison is underway Biological replica is planned
19. Peck Lab Jeff Anderson BationaShahollari Ying Wan Jenny Zhang Holly Ziobro -------------------- George Cyriac Jarrod Dye Collaborators BobE Sharp (MU) George E Davis (MU) Ian Kovalchuk (U. of Lethbridge, Canada) Manfred Heinlein (U. of Basel, Switzerland)
Editor's Notes
Our previous work on maize primary root adaptation to water deficit showed that cell elongation is maintained in the apical region of the growth zone but progressively inhibited further from the apex.
Criteria: minimum peptide per protein >=2; Protein identification probability>99.0%; Peptide identification probability>95.0%; Unique peptide probability>95.0%.
PAL is a key biosynthetic catalyst in phenylpropanoid assembly in plants and fungi, and is involved in the biosynthesis of a wide variety of secondary metabolites such as flavanoids, furanocoumarinphytoalexins and cell wall components. These compounds are important for normal growth and in responses to environmental stress.Kinase-like protein is actually found (traced to its original literature), a downstream kinase of Pto, called “Pto-interactor-1, found in tomato. SEC14 family protein: expressed in chloroplast and has transporter activity; shares a great deal of homology with Patellin 1/2, a transporter