Yerisinia enterocolitica hear bored by pigs Under cooked pork During slaughter Improper food handling Healthy adults --- gastro Interaction between bacteria and host cell (intestine ) Inside the cell of intestine yisin uses molecule syring to inject bacteria (called effector) To know how to develop Yspl interacts with mammalian protein How to determine protein interaction Using a mixture of yeast and human cell FAK is targeted by those yspl : fak regulates cellular function , affects cell movement Fak functions at focal adhesions Focal adhesion : contains many proteins (Add more info ) How yspl function at focal adhesion Hypothesis : yspl localizes to focal adhesion It doesn't interact with FAK But yes yspl localizes it focal adhesion So as a result yspl affects cell movement Yspl reduces FAK and inhibits cell migration Neutrophil chasing bacteria .