Which is NOT true for solute retention?
A) Retention time, t r, is the elapsed time between injecting the sample to the arrival of a sample component at the detector minus the dwell time between the end of the column and the detector.
B) Adjusted retention time is t r’ = t r – t m, where t r is the retention time and t m is the time for mobile phase or unretained solutes to pass through the column.
C) The relative retention, ? = r 2 , is the ratio for the adjusted retention time for two t \' r1 components of the sample, where t r2’ > t r1’ so that ? > 1.
D) The retention factor, k = t r ? t m , is the time, tr, required to elute a component
t m
K 1coefficients for components 2 and 1.minus the time, tm, for mobile phase to pass through the column divided by t m .
E) The relative retention, ?, also equals K 2 , where K 2 and K 1 are the partition coefficients for components 2 and 1.
Solution
(A) is the answer.
Reason: The absolute time taken by a compound to travel from the injector through the column to the detector is the retention time.
Dwell time will not come into the definition.
Rest all options are correct
.
Which is NOT true for solute retention- A) Retention time- tr- is the.docx
1. Which is NOT true for solute retention?
A) Retention time, t r, is the elapsed time between injecting the sample to the arrival of a sample
component at the detector minus the dwell time between the end of the column and the detector.
B) Adjusted retention time is t r’ = t r – t m, where t r is the retention time and t m is the
time for mobile phase or unretained solutes to pass through the column.
C) The relative retention, ? = r 2 , is the ratio for the adjusted retention time for two t ' r1
components of the sample, where t r2’ > t r1’ so that ? > 1.
D) The retention factor, k = t r ? t m , is the time, tr, required to elute a component
t m
K 1coefficients for components 2 and 1.minus the time, tm, for mobile phase to pass through the
column divided by t m .
E) The relative retention, ?, also equals K 2 , where K 2 and K 1 are the partition coefficients for
components 2 and 1.
Solution
(A) is the answer.
Reason: The absolute time taken by a compound to travel from the injector through the column
to the detector is the retention time.
Dwell time will not come into the definition.
Rest all options are correct
