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International Journal of Trend in Scientific Research and Development (IJTSRD)
Volume 7 Issue 1, January-February 2023 Available Online: www.ijtsrd.com e-ISSN: 2456 – 6470
@ IJTSRD | Unique Paper ID – IJTSRD52719 | Volume – 7 | Issue – 1 | January-February 2023 Page 501
Development of Nachos using Cucurbita (Pumpkin) Seed Powder,
Sorghum (Jowar) Flour, Zea Mays (Maize) Flour, and Cicer
Arietunum (Besan)
Pratik Anant Thakar, Prof. Nisha Wagh
Department of Food Technology, Parul University Applied Science, Parul University, Waghodia, Gujarat, India
ABSTRACT
The study of Nachos was carried out at the Department of Food
Technology, Parul Institute of Applied Sciences, Parul University,
Vadodara, Gujarat. This research shows the health benefits of
pumpkin seeds and the major goal was to develop Nachos that
could be enjoyed by all age groups by using the same as Pumpkin
seed have an Antimicrobial and Antioxidant property and are rich
in protein content as well. Three formulations with control sample
were formulated for the preparation of Nachos. Nachos was
developed by using pumpkin seeds, which was first roasted and
ground into powder and combined with jowar flour, maize flour,
and Chickpea flour. Roasting enhances the flavour and gives an
aroma to the food products. The treated pumpkin flour was mixed
with jowar flour, Maize flour and Chickpea flour. Spices like Chili
powder, turmeric, amchur powder, Ginger powder Coriander
powder, Chili flakes and Oregano were also used for seasoning
Nachos for better taste and flavour. Pumpkin seed powder was
added in different variations (10%, 20%, 30%) during dough
preparation. Tortillas were firstly prepared from the dough and
further it was it was fried. Difference formulation were formed to
make Nachos; Maize flour was added in 40%, 30% and 20%. The
developed nachos were further analyzed for its sensory evaluation,
physio-chemical parameters, and microbiological analysis. Dry
heat treatments were given to pumpkin seeds prior to use in
preparation of Nachos. The nachos had a 4.84% of moisture
content and 3.87% of ash content in addition it was found to have
26.01% of protein content 37.23% of carbohydrates content and
28.02% of fat content and energy 505.14kcal.
How to cite this paper: Pratik Anant
Thakar | Prof. Nisha Wagh
"Development of Nachos using
Cucurbita (Pumpkin) Seed Powder,
Sorghum (Jowar) Flour, Zea Mays
(Maize) Flour, and Cicer Arietunum
(Besan)" Published
in International
Journal of Trend in
Scientific Research
and Development
(ijtsrd), ISSN:
2456-6470,
Volume-7 | Issue-1,
February 2023, pp.501-505, URL:
www.ijtsrd.com/papers/ijtsrd52719.pdf
Copyright © 2023 by author (s) and
International Journal of Trend in
Scientific Research and Development
Journal. This is an
Open Access article
distributed under the
terms of the Creative Commons
Attribution License (CC BY 4.0)
(http://creativecommons.org/licenses/by/4.0)
KEYWORDS: Pumpkin seed, Maize
flour, Sorghum flour, Chickpea
1. INTRODUCTION
Nachos are the tortilla chips which are made by using
corn flour or wheat flour. Tortilla chips is another
name for Nachos. It is a Mexican dish consist of fried
tortilla. Nachos can be fried or baked. It is the most
important food in Mexico, America, and Colombia.
Nachos comes in different sizes and shapes of the
stripes. Shapes like Square, Round, Triangular,
Rhombus etc. Nachos can be served with or without
sauces and spices as per persons requirement. It is a
snack which have good sensory and Nutritional
characteristics due to their high Carbohydrates,
Protein and Fats. In recent year, consumption of food
habits such as Potato chips, corn flour nachos are
some of the products available in the market cannot
meet the requirement. The balance between health
and disease is influenced by social, cultural,
environmental, and economic factors, influences
which need to be kept in mind when defining and
developing public health advice for health promotion.
(L. Marletta et.al., 2010).
Family Cucurbitaceae Pumpkin belongs to the genus
Cucurbita is a versatile fruit that is used for various
food processing applications. Ranging not only in
agricultural purposes but also commercial and
ornamental sales, pumpkins are grown all over the
world for a variety of reasons with versatile uses as to
the seeds, even the flowers, cooking from the fleshy
shell most parts of which are edible. The pumpkin
seeds are by-product in the food industry. Pumpkin
IJTSRD52719
International Journal of Trend in Scientific Research and Development @ www.ijtsrd.com eISSN: 2456-6470
@ IJTSRD | Unique Paper ID – IJTSRD52719 | Volume – 7 | Issue – 1 | January-February 2023 Page 502
fruits bear numerous seeds like members of
Cucurbitaceae. They are variable in size, shape,
colour, and weight. Depending upon the polyphenolic
pigments present in it, the colour of pumpkins ranges
from golden-yellow to orange flesh (Ningthoujam
Manda Devi et.al., August 2018).
Maize or corn (Zea mays L.) belonging to family
Poaceae is known as an important annual cereal crop
of the world. Maize kernel is an edible and nutritive
part of the plant which contains vitamin C, vitamin E,
vitamin K, vitamin (thiamine) B1, vitamin (niacin)
B2, vitamin (riboflavin) B3, vitamin (pantothenic
acid) B5, vitamin (pyridoxine) B6, folic acid,
selenium, and tryptamine. Potassium is a major
nutrient present which has a good significance
because an average human diet is deficient in it
(Tajamul Rouf Shah et.al., Kumar & Jhariya et.al.
2013)
Sorghum is an excellent source of starch and protein
like other cereals and they are gluten-free cereal,
which bears significance in the present-day scenario
where the occurrence of Celiac Disease (CD), an
immunological response on the rise to gluten
intolerance. Grain sorghum contains phenolic
compounds like flavonoids, (Shahidi et. al. 1995)
which inhibit tumour development. (Huang et.al.
1992) The starches and sugars in sorghum are
released more slowly than in other cereals
(Klopfenstein et.al. 1995) and it could be beneficial to
diabetics. (Toomey et.al. 1988) Sorghum is been
consumed in various forms around the world like
baked bread, porridge, tortillas, couscous, gruel,
steam-cooked products, alcoholic, and non-alcoholic
beverages. (Arun G. Kulamarva et. al. 2009)
Chickpea (Cicer arietinum L.) plant derived from the
Fabaceae family (Nwokolo et al., 1996). A leading
manufacturer of chickpeas is India, which provides
approximately 66% of global production, (Smith, et
al., 2005; Menale, et al., 2009). Chickpeas is
reproduced from grains. Like all legumes leaves it in
good shape and it enriches the soil with nitrogen
(Biggs et al., 2007). Chickpeas occurs mainly in two
varieties Kabuli and Desi (Maheri-Sis et al., 2008).
Chickpea flour was added in Nachos for binding,
before adding chickpea flour it was observed that the
dough was not binding properly for preparation of
tortilla. It also helps in maintaining the Taste of
Nachos.
2. MATERIALS AND METHODS
The present study entitled “Development Pumpkin
seed Nachos” was carried out in the Department of
Food Technology, Parul Institute of Applied Science,
Parul University, Vadodara. This section enlists the
material used and elaborates the processing
techniques, organoleptic evaluation and analytical
procedures following during the research.
2.1. Materials
A. Raw materials used in studies
The ingredients used in preparation of Nachos were
pumpkin seed powder, Jowar flour, Maize flour,
Gram flour, Spices like turmeric, Chili powder, Salt,
Chili flakes, Coriander powder, Amchur powder,
Ginger powder and oregano procured from a local
market of Vadodara
B. Processing Equipment
Equipment required for the preparation of Nachos
are: Weighing balance, Grinder, Tawa, Spatula,
Bowl, and other utensils were obtained from Food
Processing Lab, Department of Food Technology,
Parul Institute of Applied Sciences, Parul University,
Vadodara.
2.2. Methods
A. Physio-chemical Analysis
Pumpkin seed powder, Jowar flour, Maize flour,
Gram flour, Spices like turmeric, Chili powder, Salt,
Chili flakes, Coriander powder, Amchur powder,
Ginger powder and oregano were used to prepared
Nachos were analyzed for proximate composition
i
n
c
l
u
d
i
n
g moisture, ash, protein, fat, carbohydrate, and
calories content as per the standard procedure given
by (AOAC 2005
a. Moisture content
Moisture content was estimated by drying the empty
dish and 10g of sample was weighed andgrounded in
the dish. The dish was then subjected to oven for
drying at 1350
C for 2hrs. It was again weighed after
cooling in desiccator until constant weight. The
resultant loss in weight was calculated as moisture
content.
Moisture % = Initial weight (W1)-final
weight(W2) / Initial weight (W1) × 100
b. Ash content
Ash content was determined using (AOAC 2005)
procedure. 5g of sample was weighed into pre-
weighed crucible and it was heated at low flame till all
the material was completely charred(smokeless) and
cooled. The sample was then kept in the muffle
furnace for about 2hrs, at 7000
C. It was cooled in
desiccator and weight of a sample was taken. The
procedure was repeated until two consecutive weights
were constant. The percent ash was calculated by
knowing the differencebetween the initial and final
weight.
Ash %=Weight before heating – Weight after
heating/ weight of sample × 100
International Journal of Trend in Scientific Research and Development @ www.ijtsrd.com eISSN: 2456-6470
@ IJTSRD | Unique Paper ID – IJTSRD52719 | Volume – 7 | Issue – 1 | January-February 2023 Page 503
c. Determination of Protein Carbohydrates and
Fats: The results are Protein was 26.01%,
Carbohydrate was 37.23%, and Fat was 28.02%
B. Microbial Parameter
The microbial quality of prepared Nachos was
determined. In the present study different microbial
parameters such as Total Plate Count, Yeast and
Mould were examined also the samples were
examined during the storage at ambient temperature.
a. Determination of Total Plate Count
Preparation of nutrient agar medium: 25g of
nutrient agar was added in 550ml of distilled water
and it was heated till it dissolved properly. Its
mouth was plugged with cotton and it was
sterilized in an autoclave for 20min at 121° C
Preparation of sample solution (serial dilution):
Four sterilized test tubes were taken and
numbered. In each tube 9ml of distilled water was
poured. The test tubes were plugged with cotton
plugs and were sterilized in an autoclave at 121°C
for 15min. In 9 ml distilled water of sterile test
tube 1 ml of sample was added serially
Preparation of plates: Petri plates and pipettes
were sterilized by hot air oven (dryheat treatment)
or by autoclave (moist heat treatment). Sterilized
petri dishes were taken to the laminar airflow
cabinet and ultraviolet light was switched on for
30min. After 30min UV light was switched off
and then blower was switched on, and the working
surface was cleaned by 70% 1ml sample was
poured into the plates and marked. 15-20ml of
molten media was poured into each plate. This
was done near a flame to prevent contamination of
the plate by microbes. The Plates were kept for the
solidification after swirling. The plates were then
placed into the incubator for 48hrs at 37° C and
then observed for the colonies on the plates.
b. Determination of Yeast and Mould
Preparation of potato dextrose agar medium: 11g
of Potato dextrose agar medium was added in
250ml of distilled water and it was heated to
dissolve properly. Using cotton plug the mouth
was plugged and it was sterilized in an autoclave
at 121° C for 15min.
Preparation of sample solution (serial dilution): 4
sterilized test tube were taken and numbered
accordingly. 9ml distilled water was poured in
each tube. The test tubes were closed with cotton
plugs and were sterilized inn an autoclave at
121°C for 15min. 1ml of sample was added in 9ml
distilled water of sterile test tube serially.
Preparation of plates: Petri plates and pipettes
were sterilized in hot air oven (dry heat treatment)
or by autoclave (moist heat treatment). Sterilized
petri dishes were taken to laminar air flow cabinet
and ultraviolet light was switched on for 30min.
After 30min UV light was switched off and then
blower was switched on, and the working surface
was cleaned by 70% alcohol. Plates were properly
marked and then 1ml of samples were poured into
the plates. 15-20ml of molten media was poured
into each plate. This was done near a flame to
prevent the contamination of the plate by
microbes. The Plates were kept for the
solidification after swirling. Then the plates were
kept into the incubator for 48hrs at 37°C and the
colonies were observed on the plates. The former
colonies were counted on the plate.
3. Preparation of Nachos
Formu
lation
Jowar
Flour
Pumpkin
seed
Powder
Maize
flour
Chickpea
Flour
C1 5 10 40 45
C2 5 20 20 45
C3 5 30 30 45
Table 4.1: Formulation of Nachos
The various ingredients used for the standardization
of recipe for the preparation of pumpkin seed nachos
consist of 100. Nachos were prepared in three
formulations C1; C2: C3. The amount (in grams) of
C1 Formulation was 5g of Jowar flour, 10g of
Pumpkin seed powder, 40g Maize flour, 45g Gram
flour. C2 Formulation was 5g of Jowar flour, 20g of
Pumpkin seed powder, 30g of Maize flour, 45g of
Gram flour. C3 Formulation was 5g of Jowar flour,
30g of Pumpkin seed powder, 20g of Maize flour,
45g of Gram flour. 2-3g of spices like chili powder,
pinch of salt, ginger powder, coriander powder,
Amchur powder, Chili flakes and Oregano. The
above ingredient required for making 3 formulations
weight 100g to 120g. Gram flour was used as binding
in dough preparation. The dry spices were added for
taste. Dough was prepared and tortilla was made by
using tawa for 105℃ for 2 mins. The baked tortilla
was cut into Triangular strips and fried at 120℃ for 5
minutes. The Fried tortilla was kept in Parchment
paper to remove excess of oil. Chili flakes and
Oregano were added as a seasoning after frying. The
Prepared Nachos was kept in Low Density
Polyethylene (LDPE) Zip bags. Nachos can be stored
at normal room temperature for shelf-life
International Journal of Trend in Scientific Research and Development @ www.ijtsrd.com eISSN: 2456-6470
@ IJTSRD | Unique Paper ID – IJTSRD52719 | Volume – 7 | Issue – 1 | January-February 2023 Page 504
3. RESULT AND DISCUSSION
Figure 1: Developed Nachos
The result obtained during the investigation
“Development of Nachos using Pumpkin seeds" is
discussed here. The final product was analysed for
physio-chemical analysis, microbial-analysis sensory
evaluation and stored at room temperature. Research
experiments undertaken to standardized the method
for manufacturing of Nutri-bar have been discussed
under heading
Characteristics Values
Moisture content (%) 4.84%
Ash content (%) 3.84%
Protein (%) 26.01%
Carbohydrate (%) 37.23%
Fat (%) 28.02%
Calorific value (%) 505.14 Kcal
Table 3.1.1: Proximate composition of Nachos
The moisture content in Nachos was 4.84%. Ash
content was 3.87%, Protein was 26.01%,
Carbohydrate was 37.23%, Fat was 28.02% and
calories found was 505.14 Kcal
3.1. Microbial analysis of Nacho
Total plate count (TPC) of Nachos: The mean
values for TPC of Nachos samples is 2.6x102
Log10 cfu/g.
Total yeast and mould count of Nachos: The
mean values for mould count of Nachos sample is
2x102
Log10 cfu/g.
Table 3.1.1: Sensory evaluation of produced
Nachos
Sample code C1 C2 C3
Appearance 6 9 7
Color 8 9 8
Texture 6 8 7
Taste 6 9 6
Overall Acceptability 6.5 9 7
The quality of Nachos was greatly influenced by their
flavour, texture, and taste. There were significant
changes in texture and taste of Nachos due to different
concentration Maize flour and Pumpkin seeds. The
Nachos with F1 formulation got 6 hedonic score
appearance, 8 hedonic score on Color, 8 hedonic score
on Texture, 6 hedonic score on taste and its overall
acceptability was 6.5. The bar with F1 formulation got
6.5 for overall acceptability which indicates that the
nachos is Liked slightly according to 9-point hedonic
scale. It can be because of dull taste, poor texture and
appearance and taste. F2 formulation got 9 hedonic
score appearance, 9 hedonic score on Color, 8 hedonic
score on Texture, 9 hedonic score on taste and its
overall acceptability was 9. The bar with F2
formulation got 9 for overall acceptability which
indicates Like extremely. The F3 formulation got 7
hedonic score appearance, 8 hedonic score on Color, 7
hedonic score on Texture, 7 hedonic score on taste and
its overall acceptability was 7. The bar with F3
formulation got 7 for overall acceptability which
indicates the nachos to be like moderately. Therefore,
Nutri-bar with F2 formulation was selected as the best
Nachos.
Table 3.1.2: Hedonic Scale
3.2. Packaging and Storage
The Nachos were packed in Low Density Poly-
Ethylene (LDPE) bags and can be stored in room
temperature for 3 to 4 months.
Table 3.5 Cost Estimation of per 100g Nachos
Ingredients Grams Cost
Pumpkin seeds 20g 20/-
Jowar 5g 2.5/-
Maize 30g 15/-
Besan 45g 13.5/-
Spices 5/-
TOTAL 100g 56/-
Per packet contains 20g of developed Pumpkin seed
Nachos. Hence, per 100g Nachos will produce 5
Packets out of it. Total cost estimation for 100g
Nachos is 56/-. Therefore, Nachos were sold for 20
grams at 12/-. Hence, per 100g Nacho gives 7%
profit.
International Journal of Trend in Scientific Research and Development @ www.ijtsrd.com eISSN: 2456-6470
@ IJTSRD | Unique Paper ID – IJTSRD52719 | Volume – 7 | Issue – 1 | January-February 2023 Page 505
4. Conclusion
It can be concluded from above research that from all
the formulation containing different concentration of
Maize and Pumkin seeds, F2 was found to be the best
among all the different treatments. Nachos with F2
formulation contains 4.84% moisture. 3.87% Ash,
26.01% Protein, 37.23% Carbohydrate, 28.02% Fat
and 505.14 Kcal of Energy. The prepared nachos can
provide enough energy and protein to the body. The
prepared Nachos can be eaten with or without sauce
and toppings. Nachos can be consumed by all age
groups. Nachos is called as snacks and can have
anytime while consuming meal. The ingredients for
Nachos were chosen with the intention to utilize the
waste product and develop a healthy snack. Nachos
can be stored in Low Density Poly-Ethylene (LDPE)
and can be kept in the room temperature for 3 to 4
months. The cost of the product is 12/- rupees for 20
grams per packet.
REFERENCES
[1] Agriculture and Agri-Food Canada. (2006).
Chickpeas: Situation and outlook. Bi-weekly
Bulletin. 19: 1–14.
[2] Biggs, M., McVicar, J., and Flowerdew, B.
(2007). Wielka Księga warzy. Bellona. Poland.
72–73.
[3] Huang, M.T.; Ferraro, T., Phenolics compounds
in food and cancer prevention. In Phenolic
Compounds in Food and Their Effects on
Health, II; Huang, M.T., Ho, C.T., Lee, C.Y.,
Eds.; American Chemical Society, Washington
D.C.: 1992
[4] Jambunathan, R., Subramanian, V. Grain
quality and utilization of sorghum and pearl
millet. In Biotechnology in tropical crop
improvement. Proceedings of the International
Biotechnology Workshop, ICRISAT,
Patancheru, India, 12–15 January 1987
[5] Khan, M. A., Akhtar, I. U., and Jaffery, S.
(1995). Nutritional evaluation of desi and
kabuli chickpeas and their products commonly
consumed in Pakistan. International Journal of
Food Sciences & Nutrition. 46: 215–223.
[6] Klopfenstein, C.F.; Hoseney, R.C., Nutritional
properties of sorghum and millets. In Sorghum
and Millets Chemistryand Technology; Dendy,
D.A.V., Ed.; 125 St Paul, Minn.: American
Association of Cereal Chemistry: 1995.
[7] Kumar, D., & Jhariya, N. A. (2013).
Nutritional, medicinal and economical
importance of corn: A mini review. Research
Journal of Pharmaceutical Sciences, 2, 7–8.
[8] Maheri-Sis, N., Chamani, M., Sadeghi, A-A.,
Mirza- Aghazadeh, A., and
AghajanzadehGolshani, A. (2008). Nutritional
evaluation of kabuli and desi type chickpeas
(Cicer arietinum L.) for ruminants using in
vitro gas production technique. African Journal
of Biotechnology. 7: 2946–2951.
[9] Maize—A potential source of human nutrition
and health: A review Tajamul Rouf Shah *,
Kamlesh Prasad1 and Pradyuman Kumar1
[10] Menale, K., Bekele, S., Solomon, A., Tsedeke,
A., Geoffrey, M., Setotaw, F., Million, E., and
Kebebew, A. (2009). Current Situation and
Future Outlooks of the Chickpea Sub‐sector in
Ethiopia ICRISAT and EIAR 1–35.
[11] Nandini, C.D. and Salimath, P.V. Structural
features of arabinoxylans from sorghum having
good roti-making quality. Food Chemistry
2001, 74 (4), 417–422.
[12] Nisha M Wagh, Prof. Hanmant Bodhankar,
Prof. Todkar Ashish. 2022. Development of
Nutri-bar Using Quinoa.
[13] Nutritional and Rheological Properties of
Sorghum Arun G. Kulamarva, Venkatesh R.
Sosle & G.S. Vijaya Raghavan
[14] Nwokolo, E., and Smartt, J. (1996). Food and
feed from legumes and oilseeds. In: Chapman
and Hall E-publishing. pp. 4–5.
[15] Physico-chemical characterisation of pumpkin
seeds Ningthoujam Manda Devi, RV Prasad,
Palmei Gaibimei August 2018
[16] Shahidi, F.; Naczk, M. Phenolics in cereals and
legumes. In Food phenolics; Shahidi, F.; Ed.;
Lancaster, Pa.: Technomic Publishing Co.,
1995; 9–52.
[17] Smith, V., and Jimmerson, J. (2005). Chickpeas
(Garbanzo beans). Agricultural Marketing
Policy Center (AMPC). 55.
[18] The nutritional composition of selected ethnic
foods consumed in Italynbu_1850 350..356 L.
Marletta*, E. Camilli*, A. Turrini*, P.
Scardella†, R. Spada†, L. Piombo‡, S.
Khokhar§, P. Finglas¶, E. Carnovale**
[19] Toomey, G. Sorghum as substitute. Food
enterprises for Indian woman. Int. Develop.
Res. Centre Reports 1988, 17 (3), 20–21.
[20] Waggle, D.H., Deyoe, C.W. Relationship
between protein level and amino acid
composition of sorghum grain. Feedstuffs 1966,
38, 1819

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Development of Nachos using Cucurbita Pumpkin Seed Powder, Sorghum Jowar Flour, Zea Mays Maize Flour, and Cicer Arietunum Besan

  • 1. International Journal of Trend in Scientific Research and Development (IJTSRD) Volume 7 Issue 1, January-February 2023 Available Online: www.ijtsrd.com e-ISSN: 2456 – 6470 @ IJTSRD | Unique Paper ID – IJTSRD52719 | Volume – 7 | Issue – 1 | January-February 2023 Page 501 Development of Nachos using Cucurbita (Pumpkin) Seed Powder, Sorghum (Jowar) Flour, Zea Mays (Maize) Flour, and Cicer Arietunum (Besan) Pratik Anant Thakar, Prof. Nisha Wagh Department of Food Technology, Parul University Applied Science, Parul University, Waghodia, Gujarat, India ABSTRACT The study of Nachos was carried out at the Department of Food Technology, Parul Institute of Applied Sciences, Parul University, Vadodara, Gujarat. This research shows the health benefits of pumpkin seeds and the major goal was to develop Nachos that could be enjoyed by all age groups by using the same as Pumpkin seed have an Antimicrobial and Antioxidant property and are rich in protein content as well. Three formulations with control sample were formulated for the preparation of Nachos. Nachos was developed by using pumpkin seeds, which was first roasted and ground into powder and combined with jowar flour, maize flour, and Chickpea flour. Roasting enhances the flavour and gives an aroma to the food products. The treated pumpkin flour was mixed with jowar flour, Maize flour and Chickpea flour. Spices like Chili powder, turmeric, amchur powder, Ginger powder Coriander powder, Chili flakes and Oregano were also used for seasoning Nachos for better taste and flavour. Pumpkin seed powder was added in different variations (10%, 20%, 30%) during dough preparation. Tortillas were firstly prepared from the dough and further it was it was fried. Difference formulation were formed to make Nachos; Maize flour was added in 40%, 30% and 20%. The developed nachos were further analyzed for its sensory evaluation, physio-chemical parameters, and microbiological analysis. Dry heat treatments were given to pumpkin seeds prior to use in preparation of Nachos. The nachos had a 4.84% of moisture content and 3.87% of ash content in addition it was found to have 26.01% of protein content 37.23% of carbohydrates content and 28.02% of fat content and energy 505.14kcal. How to cite this paper: Pratik Anant Thakar | Prof. Nisha Wagh "Development of Nachos using Cucurbita (Pumpkin) Seed Powder, Sorghum (Jowar) Flour, Zea Mays (Maize) Flour, and Cicer Arietunum (Besan)" Published in International Journal of Trend in Scientific Research and Development (ijtsrd), ISSN: 2456-6470, Volume-7 | Issue-1, February 2023, pp.501-505, URL: www.ijtsrd.com/papers/ijtsrd52719.pdf Copyright © 2023 by author (s) and International Journal of Trend in Scientific Research and Development Journal. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (CC BY 4.0) (http://creativecommons.org/licenses/by/4.0) KEYWORDS: Pumpkin seed, Maize flour, Sorghum flour, Chickpea 1. INTRODUCTION Nachos are the tortilla chips which are made by using corn flour or wheat flour. Tortilla chips is another name for Nachos. It is a Mexican dish consist of fried tortilla. Nachos can be fried or baked. It is the most important food in Mexico, America, and Colombia. Nachos comes in different sizes and shapes of the stripes. Shapes like Square, Round, Triangular, Rhombus etc. Nachos can be served with or without sauces and spices as per persons requirement. It is a snack which have good sensory and Nutritional characteristics due to their high Carbohydrates, Protein and Fats. In recent year, consumption of food habits such as Potato chips, corn flour nachos are some of the products available in the market cannot meet the requirement. The balance between health and disease is influenced by social, cultural, environmental, and economic factors, influences which need to be kept in mind when defining and developing public health advice for health promotion. (L. Marletta et.al., 2010). Family Cucurbitaceae Pumpkin belongs to the genus Cucurbita is a versatile fruit that is used for various food processing applications. Ranging not only in agricultural purposes but also commercial and ornamental sales, pumpkins are grown all over the world for a variety of reasons with versatile uses as to the seeds, even the flowers, cooking from the fleshy shell most parts of which are edible. The pumpkin seeds are by-product in the food industry. Pumpkin IJTSRD52719
  • 2. International Journal of Trend in Scientific Research and Development @ www.ijtsrd.com eISSN: 2456-6470 @ IJTSRD | Unique Paper ID – IJTSRD52719 | Volume – 7 | Issue – 1 | January-February 2023 Page 502 fruits bear numerous seeds like members of Cucurbitaceae. They are variable in size, shape, colour, and weight. Depending upon the polyphenolic pigments present in it, the colour of pumpkins ranges from golden-yellow to orange flesh (Ningthoujam Manda Devi et.al., August 2018). Maize or corn (Zea mays L.) belonging to family Poaceae is known as an important annual cereal crop of the world. Maize kernel is an edible and nutritive part of the plant which contains vitamin C, vitamin E, vitamin K, vitamin (thiamine) B1, vitamin (niacin) B2, vitamin (riboflavin) B3, vitamin (pantothenic acid) B5, vitamin (pyridoxine) B6, folic acid, selenium, and tryptamine. Potassium is a major nutrient present which has a good significance because an average human diet is deficient in it (Tajamul Rouf Shah et.al., Kumar & Jhariya et.al. 2013) Sorghum is an excellent source of starch and protein like other cereals and they are gluten-free cereal, which bears significance in the present-day scenario where the occurrence of Celiac Disease (CD), an immunological response on the rise to gluten intolerance. Grain sorghum contains phenolic compounds like flavonoids, (Shahidi et. al. 1995) which inhibit tumour development. (Huang et.al. 1992) The starches and sugars in sorghum are released more slowly than in other cereals (Klopfenstein et.al. 1995) and it could be beneficial to diabetics. (Toomey et.al. 1988) Sorghum is been consumed in various forms around the world like baked bread, porridge, tortillas, couscous, gruel, steam-cooked products, alcoholic, and non-alcoholic beverages. (Arun G. Kulamarva et. al. 2009) Chickpea (Cicer arietinum L.) plant derived from the Fabaceae family (Nwokolo et al., 1996). A leading manufacturer of chickpeas is India, which provides approximately 66% of global production, (Smith, et al., 2005; Menale, et al., 2009). Chickpeas is reproduced from grains. Like all legumes leaves it in good shape and it enriches the soil with nitrogen (Biggs et al., 2007). Chickpeas occurs mainly in two varieties Kabuli and Desi (Maheri-Sis et al., 2008). Chickpea flour was added in Nachos for binding, before adding chickpea flour it was observed that the dough was not binding properly for preparation of tortilla. It also helps in maintaining the Taste of Nachos. 2. MATERIALS AND METHODS The present study entitled “Development Pumpkin seed Nachos” was carried out in the Department of Food Technology, Parul Institute of Applied Science, Parul University, Vadodara. This section enlists the material used and elaborates the processing techniques, organoleptic evaluation and analytical procedures following during the research. 2.1. Materials A. Raw materials used in studies The ingredients used in preparation of Nachos were pumpkin seed powder, Jowar flour, Maize flour, Gram flour, Spices like turmeric, Chili powder, Salt, Chili flakes, Coriander powder, Amchur powder, Ginger powder and oregano procured from a local market of Vadodara B. Processing Equipment Equipment required for the preparation of Nachos are: Weighing balance, Grinder, Tawa, Spatula, Bowl, and other utensils were obtained from Food Processing Lab, Department of Food Technology, Parul Institute of Applied Sciences, Parul University, Vadodara. 2.2. Methods A. Physio-chemical Analysis Pumpkin seed powder, Jowar flour, Maize flour, Gram flour, Spices like turmeric, Chili powder, Salt, Chili flakes, Coriander powder, Amchur powder, Ginger powder and oregano were used to prepared Nachos were analyzed for proximate composition i n c l u d i n g moisture, ash, protein, fat, carbohydrate, and calories content as per the standard procedure given by (AOAC 2005 a. Moisture content Moisture content was estimated by drying the empty dish and 10g of sample was weighed andgrounded in the dish. The dish was then subjected to oven for drying at 1350 C for 2hrs. It was again weighed after cooling in desiccator until constant weight. The resultant loss in weight was calculated as moisture content. Moisture % = Initial weight (W1)-final weight(W2) / Initial weight (W1) × 100 b. Ash content Ash content was determined using (AOAC 2005) procedure. 5g of sample was weighed into pre- weighed crucible and it was heated at low flame till all the material was completely charred(smokeless) and cooled. The sample was then kept in the muffle furnace for about 2hrs, at 7000 C. It was cooled in desiccator and weight of a sample was taken. The procedure was repeated until two consecutive weights were constant. The percent ash was calculated by knowing the differencebetween the initial and final weight. Ash %=Weight before heating – Weight after heating/ weight of sample × 100
  • 3. International Journal of Trend in Scientific Research and Development @ www.ijtsrd.com eISSN: 2456-6470 @ IJTSRD | Unique Paper ID – IJTSRD52719 | Volume – 7 | Issue – 1 | January-February 2023 Page 503 c. Determination of Protein Carbohydrates and Fats: The results are Protein was 26.01%, Carbohydrate was 37.23%, and Fat was 28.02% B. Microbial Parameter The microbial quality of prepared Nachos was determined. In the present study different microbial parameters such as Total Plate Count, Yeast and Mould were examined also the samples were examined during the storage at ambient temperature. a. Determination of Total Plate Count Preparation of nutrient agar medium: 25g of nutrient agar was added in 550ml of distilled water and it was heated till it dissolved properly. Its mouth was plugged with cotton and it was sterilized in an autoclave for 20min at 121° C Preparation of sample solution (serial dilution): Four sterilized test tubes were taken and numbered. In each tube 9ml of distilled water was poured. The test tubes were plugged with cotton plugs and were sterilized in an autoclave at 121°C for 15min. In 9 ml distilled water of sterile test tube 1 ml of sample was added serially Preparation of plates: Petri plates and pipettes were sterilized by hot air oven (dryheat treatment) or by autoclave (moist heat treatment). Sterilized petri dishes were taken to the laminar airflow cabinet and ultraviolet light was switched on for 30min. After 30min UV light was switched off and then blower was switched on, and the working surface was cleaned by 70% 1ml sample was poured into the plates and marked. 15-20ml of molten media was poured into each plate. This was done near a flame to prevent contamination of the plate by microbes. The Plates were kept for the solidification after swirling. The plates were then placed into the incubator for 48hrs at 37° C and then observed for the colonies on the plates. b. Determination of Yeast and Mould Preparation of potato dextrose agar medium: 11g of Potato dextrose agar medium was added in 250ml of distilled water and it was heated to dissolve properly. Using cotton plug the mouth was plugged and it was sterilized in an autoclave at 121° C for 15min. Preparation of sample solution (serial dilution): 4 sterilized test tube were taken and numbered accordingly. 9ml distilled water was poured in each tube. The test tubes were closed with cotton plugs and were sterilized inn an autoclave at 121°C for 15min. 1ml of sample was added in 9ml distilled water of sterile test tube serially. Preparation of plates: Petri plates and pipettes were sterilized in hot air oven (dry heat treatment) or by autoclave (moist heat treatment). Sterilized petri dishes were taken to laminar air flow cabinet and ultraviolet light was switched on for 30min. After 30min UV light was switched off and then blower was switched on, and the working surface was cleaned by 70% alcohol. Plates were properly marked and then 1ml of samples were poured into the plates. 15-20ml of molten media was poured into each plate. This was done near a flame to prevent the contamination of the plate by microbes. The Plates were kept for the solidification after swirling. Then the plates were kept into the incubator for 48hrs at 37°C and the colonies were observed on the plates. The former colonies were counted on the plate. 3. Preparation of Nachos Formu lation Jowar Flour Pumpkin seed Powder Maize flour Chickpea Flour C1 5 10 40 45 C2 5 20 20 45 C3 5 30 30 45 Table 4.1: Formulation of Nachos The various ingredients used for the standardization of recipe for the preparation of pumpkin seed nachos consist of 100. Nachos were prepared in three formulations C1; C2: C3. The amount (in grams) of C1 Formulation was 5g of Jowar flour, 10g of Pumpkin seed powder, 40g Maize flour, 45g Gram flour. C2 Formulation was 5g of Jowar flour, 20g of Pumpkin seed powder, 30g of Maize flour, 45g of Gram flour. C3 Formulation was 5g of Jowar flour, 30g of Pumpkin seed powder, 20g of Maize flour, 45g of Gram flour. 2-3g of spices like chili powder, pinch of salt, ginger powder, coriander powder, Amchur powder, Chili flakes and Oregano. The above ingredient required for making 3 formulations weight 100g to 120g. Gram flour was used as binding in dough preparation. The dry spices were added for taste. Dough was prepared and tortilla was made by using tawa for 105℃ for 2 mins. The baked tortilla was cut into Triangular strips and fried at 120℃ for 5 minutes. The Fried tortilla was kept in Parchment paper to remove excess of oil. Chili flakes and Oregano were added as a seasoning after frying. The Prepared Nachos was kept in Low Density Polyethylene (LDPE) Zip bags. Nachos can be stored at normal room temperature for shelf-life
  • 4. International Journal of Trend in Scientific Research and Development @ www.ijtsrd.com eISSN: 2456-6470 @ IJTSRD | Unique Paper ID – IJTSRD52719 | Volume – 7 | Issue – 1 | January-February 2023 Page 504 3. RESULT AND DISCUSSION Figure 1: Developed Nachos The result obtained during the investigation “Development of Nachos using Pumpkin seeds" is discussed here. The final product was analysed for physio-chemical analysis, microbial-analysis sensory evaluation and stored at room temperature. Research experiments undertaken to standardized the method for manufacturing of Nutri-bar have been discussed under heading Characteristics Values Moisture content (%) 4.84% Ash content (%) 3.84% Protein (%) 26.01% Carbohydrate (%) 37.23% Fat (%) 28.02% Calorific value (%) 505.14 Kcal Table 3.1.1: Proximate composition of Nachos The moisture content in Nachos was 4.84%. Ash content was 3.87%, Protein was 26.01%, Carbohydrate was 37.23%, Fat was 28.02% and calories found was 505.14 Kcal 3.1. Microbial analysis of Nacho Total plate count (TPC) of Nachos: The mean values for TPC of Nachos samples is 2.6x102 Log10 cfu/g. Total yeast and mould count of Nachos: The mean values for mould count of Nachos sample is 2x102 Log10 cfu/g. Table 3.1.1: Sensory evaluation of produced Nachos Sample code C1 C2 C3 Appearance 6 9 7 Color 8 9 8 Texture 6 8 7 Taste 6 9 6 Overall Acceptability 6.5 9 7 The quality of Nachos was greatly influenced by their flavour, texture, and taste. There were significant changes in texture and taste of Nachos due to different concentration Maize flour and Pumpkin seeds. The Nachos with F1 formulation got 6 hedonic score appearance, 8 hedonic score on Color, 8 hedonic score on Texture, 6 hedonic score on taste and its overall acceptability was 6.5. The bar with F1 formulation got 6.5 for overall acceptability which indicates that the nachos is Liked slightly according to 9-point hedonic scale. It can be because of dull taste, poor texture and appearance and taste. F2 formulation got 9 hedonic score appearance, 9 hedonic score on Color, 8 hedonic score on Texture, 9 hedonic score on taste and its overall acceptability was 9. The bar with F2 formulation got 9 for overall acceptability which indicates Like extremely. The F3 formulation got 7 hedonic score appearance, 8 hedonic score on Color, 7 hedonic score on Texture, 7 hedonic score on taste and its overall acceptability was 7. The bar with F3 formulation got 7 for overall acceptability which indicates the nachos to be like moderately. Therefore, Nutri-bar with F2 formulation was selected as the best Nachos. Table 3.1.2: Hedonic Scale 3.2. Packaging and Storage The Nachos were packed in Low Density Poly- Ethylene (LDPE) bags and can be stored in room temperature for 3 to 4 months. Table 3.5 Cost Estimation of per 100g Nachos Ingredients Grams Cost Pumpkin seeds 20g 20/- Jowar 5g 2.5/- Maize 30g 15/- Besan 45g 13.5/- Spices 5/- TOTAL 100g 56/- Per packet contains 20g of developed Pumpkin seed Nachos. Hence, per 100g Nachos will produce 5 Packets out of it. Total cost estimation for 100g Nachos is 56/-. Therefore, Nachos were sold for 20 grams at 12/-. Hence, per 100g Nacho gives 7% profit.
  • 5. International Journal of Trend in Scientific Research and Development @ www.ijtsrd.com eISSN: 2456-6470 @ IJTSRD | Unique Paper ID – IJTSRD52719 | Volume – 7 | Issue – 1 | January-February 2023 Page 505 4. Conclusion It can be concluded from above research that from all the formulation containing different concentration of Maize and Pumkin seeds, F2 was found to be the best among all the different treatments. Nachos with F2 formulation contains 4.84% moisture. 3.87% Ash, 26.01% Protein, 37.23% Carbohydrate, 28.02% Fat and 505.14 Kcal of Energy. The prepared nachos can provide enough energy and protein to the body. The prepared Nachos can be eaten with or without sauce and toppings. Nachos can be consumed by all age groups. Nachos is called as snacks and can have anytime while consuming meal. The ingredients for Nachos were chosen with the intention to utilize the waste product and develop a healthy snack. Nachos can be stored in Low Density Poly-Ethylene (LDPE) and can be kept in the room temperature for 3 to 4 months. The cost of the product is 12/- rupees for 20 grams per packet. REFERENCES [1] Agriculture and Agri-Food Canada. (2006). Chickpeas: Situation and outlook. Bi-weekly Bulletin. 19: 1–14. [2] Biggs, M., McVicar, J., and Flowerdew, B. (2007). Wielka Księga warzy. Bellona. Poland. 72–73. [3] Huang, M.T.; Ferraro, T., Phenolics compounds in food and cancer prevention. In Phenolic Compounds in Food and Their Effects on Health, II; Huang, M.T., Ho, C.T., Lee, C.Y., Eds.; American Chemical Society, Washington D.C.: 1992 [4] Jambunathan, R., Subramanian, V. Grain quality and utilization of sorghum and pearl millet. In Biotechnology in tropical crop improvement. Proceedings of the International Biotechnology Workshop, ICRISAT, Patancheru, India, 12–15 January 1987 [5] Khan, M. A., Akhtar, I. U., and Jaffery, S. (1995). Nutritional evaluation of desi and kabuli chickpeas and their products commonly consumed in Pakistan. International Journal of Food Sciences & Nutrition. 46: 215–223. [6] Klopfenstein, C.F.; Hoseney, R.C., Nutritional properties of sorghum and millets. In Sorghum and Millets Chemistryand Technology; Dendy, D.A.V., Ed.; 125 St Paul, Minn.: American Association of Cereal Chemistry: 1995. [7] Kumar, D., & Jhariya, N. A. (2013). Nutritional, medicinal and economical importance of corn: A mini review. Research Journal of Pharmaceutical Sciences, 2, 7–8. [8] Maheri-Sis, N., Chamani, M., Sadeghi, A-A., Mirza- Aghazadeh, A., and AghajanzadehGolshani, A. (2008). Nutritional evaluation of kabuli and desi type chickpeas (Cicer arietinum L.) for ruminants using in vitro gas production technique. African Journal of Biotechnology. 7: 2946–2951. [9] Maize—A potential source of human nutrition and health: A review Tajamul Rouf Shah *, Kamlesh Prasad1 and Pradyuman Kumar1 [10] Menale, K., Bekele, S., Solomon, A., Tsedeke, A., Geoffrey, M., Setotaw, F., Million, E., and Kebebew, A. (2009). Current Situation and Future Outlooks of the Chickpea Sub‐sector in Ethiopia ICRISAT and EIAR 1–35. [11] Nandini, C.D. and Salimath, P.V. Structural features of arabinoxylans from sorghum having good roti-making quality. Food Chemistry 2001, 74 (4), 417–422. [12] Nisha M Wagh, Prof. Hanmant Bodhankar, Prof. Todkar Ashish. 2022. Development of Nutri-bar Using Quinoa. [13] Nutritional and Rheological Properties of Sorghum Arun G. Kulamarva, Venkatesh R. Sosle & G.S. Vijaya Raghavan [14] Nwokolo, E., and Smartt, J. (1996). Food and feed from legumes and oilseeds. In: Chapman and Hall E-publishing. pp. 4–5. [15] Physico-chemical characterisation of pumpkin seeds Ningthoujam Manda Devi, RV Prasad, Palmei Gaibimei August 2018 [16] Shahidi, F.; Naczk, M. Phenolics in cereals and legumes. In Food phenolics; Shahidi, F.; Ed.; Lancaster, Pa.: Technomic Publishing Co., 1995; 9–52. [17] Smith, V., and Jimmerson, J. (2005). Chickpeas (Garbanzo beans). Agricultural Marketing Policy Center (AMPC). 55. [18] The nutritional composition of selected ethnic foods consumed in Italynbu_1850 350..356 L. Marletta*, E. Camilli*, A. Turrini*, P. Scardella†, R. Spada†, L. Piombo‡, S. Khokhar§, P. Finglas¶, E. Carnovale** [19] Toomey, G. Sorghum as substitute. Food enterprises for Indian woman. Int. Develop. Res. Centre Reports 1988, 17 (3), 20–21. [20] Waggle, D.H., Deyoe, C.W. Relationship between protein level and amino acid composition of sorghum grain. Feedstuffs 1966, 38, 1819