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@ IJTSRD | Available Online @ www.ijtsrd.com
ISSN No: 2456
International
Research
UGC Approved International Open Access Journal
Study on Nutritious Multigrain Weaning
S.M.Patel College Of HomeScience,
Vallabh Vidyanagar
ABSTRACT
The study was on the development of weaning food
based on wheat, chickpea and ragi and adapting the
Malting, Germinating and Popping technology. The
composition was done for the physico-
nutritional quality of malted, germinated and popped
weaning mix with the control weaning mix with
similar composition. The results suggest that the
weaning foods based on wheat, chickpea and ragi are
nutritionally balanced and possess good growth
promoting quality. The traditional technologies such
as Malting, Germinating and Popping of cereals and
legumes could be easily adapted to prepared weaning
and supplementary foods from wheat, chickpea and
ragi. Malting, Germinating and Popping have a high
potential for developing low cost weaning and
supplementary foods.
INTRODUCTION
Having a baby at home is a wonderful experience.
Looking after the baby and caring for it is an even
more fulfilling experience. There are several
important stages in an infant's growth. But one of the
most important aspects, and often misunderstood
stage, is that of weaning. The introduction to solid
feeding and the gradual replacement of milk by solid
food as the main source of nutrition is the process
known as weaning. Weaning is a period of transition
for the infant during which the diet changes in term
of consistency and source (Usha et al., 2010). At six
months, a baby's digestive system has matured
enough to cope with solid food, and other
developmental changes (such as the ability to bite and
@ IJTSRD | Available Online @ www.ijtsrd.com | Volume – 1 | Issue – 5
ISSN No: 2456 - 6470 | www.ijtsrd.com | Volume
International Journal of Trend in Scientific
Research and Development (IJTSRD)
UGC Approved International Open Access Journal
Study on Nutritious Multigrain Weaning Mix
Dr. Bhavana Chauhan
S.M.Patel College Of HomeScience,
Vallabh Vidyanagar, Gujarat, India
development of weaning food
based on wheat, chickpea and ragi and adapting the
Malting, Germinating and Popping technology. The
- chemical and
nutritional quality of malted, germinated and popped
trol weaning mix with
similar composition. The results suggest that the
weaning foods based on wheat, chickpea and ragi are
nutritionally balanced and possess good growth
promoting quality. The traditional technologies such
ing of cereals and
legumes could be easily adapted to prepared weaning
and supplementary foods from wheat, chickpea and
ragi. Malting, Germinating and Popping have a high
potential for developing low cost weaning and
Having a baby at home is a wonderful experience.
Looking after the baby and caring for it is an even
more fulfilling experience. There are several
important stages in an infant's growth. But one of the
most important aspects, and often misunderstood
is that of weaning. The introduction to solid
feeding and the gradual replacement of milk by solid
food as the main source of nutrition is the process
known as weaning. Weaning is a period of transition
for the infant during which the diet changes in terms
of consistency and source (Usha et al., 2010). At six
months, a baby's digestive system has matured
enough to cope with solid food, and other
developmental changes (such as the ability to bite and
chew) mean your baby is ready to experience new
tastes and textures.
From a liquid milk-based diet, the child is gradually
introduced to semi-solid foods. Such semi
are referred to as complementary foods. A
complementary food should ideally be easily
digestible, have high energy density and low b
(Ezeji and Ojimelukwe, 1993). Between the ages of 6
8 months pureed and mashed foods can be introduced
and given from a cup or bowl.
However, the capacity of a weaning diet to meet the
protein and energy requirements of infants depends on
its nutritional quality as well as its dietary bulk. This
can be achieved through legume supplementation of
cereal-based weaning foods. However, their role
appears to be limited because of several factors
including low protein and starch digestibility, poor
mineral bioavailability and high anti
factors (Kamchan et al., 2004, Negi et al., 2001). It
has been reported that protein and thiamin (Sattar et
al., 1989, Savelkoul 1992) mineral bioavailability and
protein and starch digestibility (Preet and Punia,
2000) increased, whereas phytic acid and tannin
decreased during germination of legumes.
Legumes are known to contain lysine in a quantity
that exceeds the requirements for human but with the
low content of sulphur amino acids. Cereals, on the
other hand, are high in the sulphur amino acids but
deficient in lysine. A mutual complementation of
amino acids and consequent improvement in protein
quality is therefore achieved when legumes are
blended with cereals in the right proportions. Cereals
form the major part of most weaning mixes and
contribute to 70-80% of daily energy intake (Mahajan
Page: 184
6470 | www.ijtsrd.com | Volume - 1 | Issue – 5
Scientific
(IJTSRD)
UGC Approved International Open Access Journal
Mix
chew) mean your baby is ready to experience new
based diet, the child is gradually
solid foods. Such semi-solid foods
are referred to as complementary foods. A
complementary food should ideally be easily
digestible, have high energy density and low bulk
(Ezeji and Ojimelukwe, 1993). Between the ages of 6-
8 months pureed and mashed foods can be introduced
and given from a cup or bowl.
However, the capacity of a weaning diet to meet the
protein and energy requirements of infants depends on
al quality as well as its dietary bulk. This
can be achieved through legume supplementation of
based weaning foods. However, their role
appears to be limited because of several factors
including low protein and starch digestibility, poor
availability and high anti-nutritional
factors (Kamchan et al., 2004, Negi et al., 2001). It
has been reported that protein and thiamin (Sattar et
al., 1989, Savelkoul 1992) mineral bioavailability and
protein and starch digestibility (Preet and Punia,
0) increased, whereas phytic acid and tannin
decreased during germination of legumes.
Legumes are known to contain lysine in a quantity
that exceeds the requirements for human but with the
low content of sulphur amino acids. Cereals, on the
e high in the sulphur amino acids but
deficient in lysine. A mutual complementation of
amino acids and consequent improvement in protein
quality is therefore achieved when legumes are
blended with cereals in the right proportions. Cereals
rt of most weaning mixes and
80% of daily energy intake (Mahajan
International Journal of Trend in Scientific Research and Development (IJTSRD) ISSN: 2456-6470
@ IJTSRD | Available Online @ www.ijtsrd.com | Volume – 1 | Issue – 5 Page: 185
and Chattopadhey, 2000). However, they are deficient
in one or two essential amino acids. Legumes are
largely replacing milk and other sources of animal
proteins, which are expensive and not readily
available in India as suitable substitutes for high
quality protein. Therefore, supplementing with
legumes and other nutrient dense food products
improves the nutritional quality of cereals by
complementing their limited amino acids, lysine and
tryptophan (Hulse, 1991).
MATERIALS AND METHODS
Wheat, chickpea and ragi purchased from a local
market (Anand, India) were cleaned and used for the
studies. Three different processing techniques
(Malting, Germination & Popping) used for the
development of processed multi grain weaning mix.
A) Malting:
Wheat, chickpea and ragi were soaked separately in
water for overnight and then after removal of water
they were sun dried, till they become completely dry
and flour was prepared using grinding mill.
B) Germination:
Wheat, Chickpea and Ragi were soaked overnight in
water and then packed individually in wet muslin
cloth for sprouting. After sprouting they were
sundried and then flour was made using the grinding
mill.
C) Popping:
Wheat, Chickpea and Ragi were roasted in dry sand
individually at high temperature for short period of
time, till they become completely roasted and after
that flour was prepared using the grinding mill.
Nestle Cerelac Stage 1(6+months) Wheat based
product was used as a control product. The
experimental product included the addition of
different proportion of cereals and pulse combination.
The multigrain weaning mix was prepared from the
different processed flours (i.e. Malting, Germination
and Popping).
Development of Standard Product:
Ingredients
Experimental Weaning Mix
Control
(g)
A
Without Process (g)
B
Malted
(g)
C
Germinated
(g)
D
Popped
(g)
Nestle Cerelac 100 - - - -
Wheat flour - 25 25 25 25
Chickpea flour - 10 10 10 10
Ragi flour - 25 25 25 25
Skimmed Milk Powder - 30 30 30 30
Crystal sugar powder - 10 10 10 10
For Standardization of experimental product the
instant weaning mix powder was mixed with
lukewarm water with proper and continuous stirring
getting the proper homogeneous mixture. After this
the control and experimental product which needed to
be analyzed in the laboratory test. In this part of the
study, both control as well as experimental products
were undergone for Nutrient Analysis of fat and ash
contents and were estimated by standard AOAC, 1984
methods. The nitrogen content was estimated by
Khjeldhal (1883) method. Carbohydrate content was
Analysed by Anthrone method. (Hedge and Hofreiter,
1962). The samples were ash in a muffle furnace and
then ash solution was prepared from this ash solution
the Iron was estimated by colorimetrically using the
Dipyridyl method of Ramsay (1954). Calcium was
analyzed by precipitation as calcium oxalate and
subsequent titration by potassium permanganate by
the method of titrimetric method of Clark and Collip
(1925). Phosphorus was estimated by colorimetric
method of Fiske and Subbarow (1925). Insoluble and
soluble dietary fibre was estimated by AOAC method.
International Journal of Trend in Scientific Research and Development (IJTSRD) ISSN: 2456-6470
@ IJTSRD | Available Online @ www.ijtsrd.com | Volume – 1 | Issue – 5 Page: 186
Zinc was determined by titrimetric method of Jeffery
et al. (1889).
After that Physical Analysis was carried out by The
Water Holding Capacity (WHC) of the samples were
determined by the centrifuge technique described by
percent dispersibility was determined by the method
of Gandhi and Srivastava (2007).
The analysis was carried out in triplicates for all
determinations. The mean, standard deviation of
means, standard error of means were calculated. A
multiple comparison procedure of the means was
performed by the T test. The Correlation Coefficients
were computed. Significance of the differences was
defined as p<0.05.
RESULTS AND DISCCUSION
Table 1: Nutrient Content of Control and Experimental Weaning Mix
Nutrients
Weaning Mix
Cerelac
(Control)
Experimental
A B C D
Ash (g%)
2.763
±0.064
2.725
±0.022
2.655
±0.035
2.470
±0.119
2.780
±0.083
Calcium (mg%)
539.259
±15.983
615.185**
±15.072
550.370
±6.676
185.555**
±7.856
402.222**
±3.928
Phosphorus (mg%)
296.305
±2.216
258.947**
±2.292
292.820*
±4.847
281.171*
±5.100
288.396*
±2.967
Iron (mg%)
14.590
±0.794
27.360**
±1.431
29.970**
±0..670
28.906**
±0.605
33.801**
±0.618
Zinc (mg %)
0.553
±0.009
1.315**
±0.008
1.466**
±0.009
1.358**
±0.008
0.956**
±0.003
Carbohydrate (g%)
96.000
±0.333
54.888**
±0.888
63.555**
±0.376
63.555**
±0.376
70.888**
±0.200
Fat (g%)
4.666
±0.666
4.666
±0.421
5.333
±0.421
3.666
±0.333
7.333*
±0.421
Protein (g%)
13.883
±0.583
18.433
±0816
20.008*
±0.291
14.466
±0.583
21.350*
±0.534
Crude fiber (g%)
0.753
±0.003
1.703**
±0.003
1.996**
±0.016
1.860**
±0.03
1.833**
±0.016
Water Holding
Capacity (g%)
1.822**
±0.022
0.611**
±0.030
0.866**
±0.072
0.944**
±0.024
0.955**
±0.024
Control : Cerelac Weaning Mix, A : Weaning Mix without processing
B: Malted Weaning Mix, C : Germinated Weaning Mix, D : Popped Weaning Mix
Mean of three replication ±SEM, Value sharing a common superscript within a control are not significant
different, *p<0.05= significant difference, **p<0.01=highly significant difference, NS = Non significant
difference.
Table 1 shows the nutrient contents i.e. Ash, Calcium,
Phosphorus, Iron, Zinc, Carbohydrate, Fat, Protein,
Crude fibre and Water holding Capacity of Control
and experimental weaning mix. The calcium content
was found higher in the without process weaning mix
and lowest in the germinated weaning mix.
Phosphorus value of all experimental weaning mix
was found lower compared to the Cerelac. The Iron
and Zinc contents of all the experimental weaning mix
were found higher compared to the Cerelac and it
shows highly significant difference (p<0.01) also.
The Carbohydrate was found lower in all the
International Journal of Trend in Scientific Research and Development (IJTSRD) ISSN: 2456-6470
@ IJTSRD | Available Online @ www.ijtsrd.com | Volume – 1 | Issue – 5 Page: 187
experimental weaning mix compared to the Cerelac
and they were significantly differ (p<0.01) too.
Protein content was also found slightly higher
compared to the Cerelac and the significant difference
(p<0.05) was seen in the Malted and Popped weaning
mix in comparison of Cerelac. The Water holding
capacity of all the experimental weaning mix was
lowest compared to the Cerelac as the Cerelac
contained higher amount of carbohydrate.
CONCLUSION
The Calcium was higher in A sample i.e. without
process weaning mix. The Phosphorous and Zinc
were higher in the B sample i.e. Malted weaning mix.
Whereas the Iron, Carbohydrate, Fat and Protein were
higher in the D sample i.e. popped weaning mix. The
Crude Fibre was found higher in the all the
experimental weaning mix compared of the control
sample i.e. Cerelac weaning mix.
In Conclusion of the study results suggest that the
weaning foods based on wheat, chickpea and ragi are
nutritionally balanced and possess good growth
promoting quality. The traditional technologies such
as Malting, Germinating and Popping of cereals and
legumes could be easily adapted to prepared weaning
and supplementary foods from wheat, chickpea and
ragi. Malting, Germinating and Popping have a high
potential for developing low cost weaning and
supplementary foods.
REFRENCES
 AOAC (1984), “Official Method of Analysis”
14th Edn. Associationn of official analytical
chemists, Washington DC.
 Clark F. P. and Collip J. B, (1925),”Determination
of calcium by titremetric method”, journal of
biological chemistry, 63,461.
 Ezeji C. and Ojimelukwe P.C.(1993),'' Effect of
fermentation on nutritional quality and functional
properties of infant food formulations prepared
from pambara groundnut, fruit dip pumpkin and
millet seeds'', Plant Foods Hum Nutr., 44 : 267-
276.
 Fiske C. H. and Subbarow (1925),“Colorimetric
Determination of phosphorus”, journal of
biological chemistry, 66 : 375-400.
 Gandhi, A.P. and Srivastava, T. (2007), “Studies
on the production of protein isolates from defatted
sesame seed (Sesamum indicum) flour and their
nutritional profile, Asian Food Journal, 14(3),
175-180.
 Hedge, J.E. and Hefreiter, B.T. (1962), “Methods
in carbohydrate chemistry”, Vol. 17 (Eds.),
Whistler R.L. and BeMiller, J.N., Academic Press,
New York, p.420.
 Hulse J.H.(1991),''Nature, composition and
utilization of grain legumes'', In: Uses of Tropical
Legumes: Proceedings of a Consultants’ Meeting.,
11-27. ICRISAT Center, ICRISAT, Patancheru,
A.P. 502-324, India.
 Jaffery G. H., Bassett J., Mendham
J.(1989),”Vogel's quantitative analysis of
chemicals”, Hangman Harlow.,: 5 284-294.
 Kamchan A., Puwastien P., Sirichakwal P.P. and
Kongkachuichai R.(2004),''In vitro calcium
bioavilability of vegetables, legumes and seeds'',
J. Food Composition and Analysis., 17 : 311-32.
 Kjeldahl, J. (1883), New method for the
determination of nitrogen in organic substances,
Zeitschrift fur analytische chemie, 22(1): 366-383.
 Mahajan P.V. and Chattopadhey
P.K.(2000),''Development of chemically leavened
cereal legume based instant mix (dhokla)'',J Food
Technol., 37 : 459-464.
 Negi A., Boora P. and Khetarpaul
N.(2001),''Starch and protein digestibility of
newly released moth bean cultivars: Effect of
soaking, dehulling, germination and pressure
cooking'',Nahrung., 45(4) : 251-254.
 Preet K., Punia D.(2000), ''Antinutrients and
digestibility (in vitro) of soaked, dehulled and
germinated cowpeas'', Nutr. Health., 14(2) : 109-
117.
 Ramsay W. N. M.(1969),''Method of iron
estimation'', journal of biological chemistry,17,
Practical clinical biochemistry – 4th edition.
 Sattar A., Durrani S.K., Mahmood F., Ahnad A.
and Khan I.(1989), ''Effect of soaking and
germination temperatures on selected nutrients
and antinutrients of mung bean'', Food Chem., 34 :
111-120.
 Savelkoul F.H.M.G., Vanderpeol A.F.B. and
Tamminga S.(1992),''The presence and
inactivation of trypsin inhibitors, tannins, lectins
and amylase inhibitors in legume seeds during
germination. A review'',Plant Foods for Human
Nut., 42 : 71-85.
 Usha R., Lakshni M. and Ranjani M.(2010), ''
Nutritional, Sensory and Physical Analysis of
Pumpkin Flour Incorporated into Weaning Mix'',
Mal J Nutr., 16(3) : 379-387.

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Study on Nutritious Multigrain Weaning Mix

  • 1. @ IJTSRD | Available Online @ www.ijtsrd.com ISSN No: 2456 International Research UGC Approved International Open Access Journal Study on Nutritious Multigrain Weaning S.M.Patel College Of HomeScience, Vallabh Vidyanagar ABSTRACT The study was on the development of weaning food based on wheat, chickpea and ragi and adapting the Malting, Germinating and Popping technology. The composition was done for the physico- nutritional quality of malted, germinated and popped weaning mix with the control weaning mix with similar composition. The results suggest that the weaning foods based on wheat, chickpea and ragi are nutritionally balanced and possess good growth promoting quality. The traditional technologies such as Malting, Germinating and Popping of cereals and legumes could be easily adapted to prepared weaning and supplementary foods from wheat, chickpea and ragi. Malting, Germinating and Popping have a high potential for developing low cost weaning and supplementary foods. INTRODUCTION Having a baby at home is a wonderful experience. Looking after the baby and caring for it is an even more fulfilling experience. There are several important stages in an infant's growth. But one of the most important aspects, and often misunderstood stage, is that of weaning. The introduction to solid feeding and the gradual replacement of milk by solid food as the main source of nutrition is the process known as weaning. Weaning is a period of transition for the infant during which the diet changes in term of consistency and source (Usha et al., 2010). At six months, a baby's digestive system has matured enough to cope with solid food, and other developmental changes (such as the ability to bite and @ IJTSRD | Available Online @ www.ijtsrd.com | Volume – 1 | Issue – 5 ISSN No: 2456 - 6470 | www.ijtsrd.com | Volume International Journal of Trend in Scientific Research and Development (IJTSRD) UGC Approved International Open Access Journal Study on Nutritious Multigrain Weaning Mix Dr. Bhavana Chauhan S.M.Patel College Of HomeScience, Vallabh Vidyanagar, Gujarat, India development of weaning food based on wheat, chickpea and ragi and adapting the Malting, Germinating and Popping technology. The - chemical and nutritional quality of malted, germinated and popped trol weaning mix with similar composition. The results suggest that the weaning foods based on wheat, chickpea and ragi are nutritionally balanced and possess good growth promoting quality. The traditional technologies such ing of cereals and legumes could be easily adapted to prepared weaning and supplementary foods from wheat, chickpea and ragi. Malting, Germinating and Popping have a high potential for developing low cost weaning and Having a baby at home is a wonderful experience. Looking after the baby and caring for it is an even more fulfilling experience. There are several important stages in an infant's growth. But one of the most important aspects, and often misunderstood is that of weaning. The introduction to solid feeding and the gradual replacement of milk by solid food as the main source of nutrition is the process known as weaning. Weaning is a period of transition for the infant during which the diet changes in terms of consistency and source (Usha et al., 2010). At six months, a baby's digestive system has matured enough to cope with solid food, and other developmental changes (such as the ability to bite and chew) mean your baby is ready to experience new tastes and textures. From a liquid milk-based diet, the child is gradually introduced to semi-solid foods. Such semi are referred to as complementary foods. A complementary food should ideally be easily digestible, have high energy density and low b (Ezeji and Ojimelukwe, 1993). Between the ages of 6 8 months pureed and mashed foods can be introduced and given from a cup or bowl. However, the capacity of a weaning diet to meet the protein and energy requirements of infants depends on its nutritional quality as well as its dietary bulk. This can be achieved through legume supplementation of cereal-based weaning foods. However, their role appears to be limited because of several factors including low protein and starch digestibility, poor mineral bioavailability and high anti factors (Kamchan et al., 2004, Negi et al., 2001). It has been reported that protein and thiamin (Sattar et al., 1989, Savelkoul 1992) mineral bioavailability and protein and starch digestibility (Preet and Punia, 2000) increased, whereas phytic acid and tannin decreased during germination of legumes. Legumes are known to contain lysine in a quantity that exceeds the requirements for human but with the low content of sulphur amino acids. Cereals, on the other hand, are high in the sulphur amino acids but deficient in lysine. A mutual complementation of amino acids and consequent improvement in protein quality is therefore achieved when legumes are blended with cereals in the right proportions. Cereals form the major part of most weaning mixes and contribute to 70-80% of daily energy intake (Mahajan Page: 184 6470 | www.ijtsrd.com | Volume - 1 | Issue – 5 Scientific (IJTSRD) UGC Approved International Open Access Journal Mix chew) mean your baby is ready to experience new based diet, the child is gradually solid foods. Such semi-solid foods are referred to as complementary foods. A complementary food should ideally be easily digestible, have high energy density and low bulk (Ezeji and Ojimelukwe, 1993). Between the ages of 6- 8 months pureed and mashed foods can be introduced and given from a cup or bowl. However, the capacity of a weaning diet to meet the protein and energy requirements of infants depends on al quality as well as its dietary bulk. This can be achieved through legume supplementation of based weaning foods. However, their role appears to be limited because of several factors including low protein and starch digestibility, poor availability and high anti-nutritional factors (Kamchan et al., 2004, Negi et al., 2001). It has been reported that protein and thiamin (Sattar et al., 1989, Savelkoul 1992) mineral bioavailability and protein and starch digestibility (Preet and Punia, 0) increased, whereas phytic acid and tannin decreased during germination of legumes. Legumes are known to contain lysine in a quantity that exceeds the requirements for human but with the low content of sulphur amino acids. Cereals, on the e high in the sulphur amino acids but deficient in lysine. A mutual complementation of amino acids and consequent improvement in protein quality is therefore achieved when legumes are blended with cereals in the right proportions. Cereals rt of most weaning mixes and 80% of daily energy intake (Mahajan
  • 2. International Journal of Trend in Scientific Research and Development (IJTSRD) ISSN: 2456-6470 @ IJTSRD | Available Online @ www.ijtsrd.com | Volume – 1 | Issue – 5 Page: 185 and Chattopadhey, 2000). However, they are deficient in one or two essential amino acids. Legumes are largely replacing milk and other sources of animal proteins, which are expensive and not readily available in India as suitable substitutes for high quality protein. Therefore, supplementing with legumes and other nutrient dense food products improves the nutritional quality of cereals by complementing their limited amino acids, lysine and tryptophan (Hulse, 1991). MATERIALS AND METHODS Wheat, chickpea and ragi purchased from a local market (Anand, India) were cleaned and used for the studies. Three different processing techniques (Malting, Germination & Popping) used for the development of processed multi grain weaning mix. A) Malting: Wheat, chickpea and ragi were soaked separately in water for overnight and then after removal of water they were sun dried, till they become completely dry and flour was prepared using grinding mill. B) Germination: Wheat, Chickpea and Ragi were soaked overnight in water and then packed individually in wet muslin cloth for sprouting. After sprouting they were sundried and then flour was made using the grinding mill. C) Popping: Wheat, Chickpea and Ragi were roasted in dry sand individually at high temperature for short period of time, till they become completely roasted and after that flour was prepared using the grinding mill. Nestle Cerelac Stage 1(6+months) Wheat based product was used as a control product. The experimental product included the addition of different proportion of cereals and pulse combination. The multigrain weaning mix was prepared from the different processed flours (i.e. Malting, Germination and Popping). Development of Standard Product: Ingredients Experimental Weaning Mix Control (g) A Without Process (g) B Malted (g) C Germinated (g) D Popped (g) Nestle Cerelac 100 - - - - Wheat flour - 25 25 25 25 Chickpea flour - 10 10 10 10 Ragi flour - 25 25 25 25 Skimmed Milk Powder - 30 30 30 30 Crystal sugar powder - 10 10 10 10 For Standardization of experimental product the instant weaning mix powder was mixed with lukewarm water with proper and continuous stirring getting the proper homogeneous mixture. After this the control and experimental product which needed to be analyzed in the laboratory test. In this part of the study, both control as well as experimental products were undergone for Nutrient Analysis of fat and ash contents and were estimated by standard AOAC, 1984 methods. The nitrogen content was estimated by Khjeldhal (1883) method. Carbohydrate content was Analysed by Anthrone method. (Hedge and Hofreiter, 1962). The samples were ash in a muffle furnace and then ash solution was prepared from this ash solution the Iron was estimated by colorimetrically using the Dipyridyl method of Ramsay (1954). Calcium was analyzed by precipitation as calcium oxalate and subsequent titration by potassium permanganate by the method of titrimetric method of Clark and Collip (1925). Phosphorus was estimated by colorimetric method of Fiske and Subbarow (1925). Insoluble and soluble dietary fibre was estimated by AOAC method.
  • 3. International Journal of Trend in Scientific Research and Development (IJTSRD) ISSN: 2456-6470 @ IJTSRD | Available Online @ www.ijtsrd.com | Volume – 1 | Issue – 5 Page: 186 Zinc was determined by titrimetric method of Jeffery et al. (1889). After that Physical Analysis was carried out by The Water Holding Capacity (WHC) of the samples were determined by the centrifuge technique described by percent dispersibility was determined by the method of Gandhi and Srivastava (2007). The analysis was carried out in triplicates for all determinations. The mean, standard deviation of means, standard error of means were calculated. A multiple comparison procedure of the means was performed by the T test. The Correlation Coefficients were computed. Significance of the differences was defined as p<0.05. RESULTS AND DISCCUSION Table 1: Nutrient Content of Control and Experimental Weaning Mix Nutrients Weaning Mix Cerelac (Control) Experimental A B C D Ash (g%) 2.763 ±0.064 2.725 ±0.022 2.655 ±0.035 2.470 ±0.119 2.780 ±0.083 Calcium (mg%) 539.259 ±15.983 615.185** ±15.072 550.370 ±6.676 185.555** ±7.856 402.222** ±3.928 Phosphorus (mg%) 296.305 ±2.216 258.947** ±2.292 292.820* ±4.847 281.171* ±5.100 288.396* ±2.967 Iron (mg%) 14.590 ±0.794 27.360** ±1.431 29.970** ±0..670 28.906** ±0.605 33.801** ±0.618 Zinc (mg %) 0.553 ±0.009 1.315** ±0.008 1.466** ±0.009 1.358** ±0.008 0.956** ±0.003 Carbohydrate (g%) 96.000 ±0.333 54.888** ±0.888 63.555** ±0.376 63.555** ±0.376 70.888** ±0.200 Fat (g%) 4.666 ±0.666 4.666 ±0.421 5.333 ±0.421 3.666 ±0.333 7.333* ±0.421 Protein (g%) 13.883 ±0.583 18.433 ±0816 20.008* ±0.291 14.466 ±0.583 21.350* ±0.534 Crude fiber (g%) 0.753 ±0.003 1.703** ±0.003 1.996** ±0.016 1.860** ±0.03 1.833** ±0.016 Water Holding Capacity (g%) 1.822** ±0.022 0.611** ±0.030 0.866** ±0.072 0.944** ±0.024 0.955** ±0.024 Control : Cerelac Weaning Mix, A : Weaning Mix without processing B: Malted Weaning Mix, C : Germinated Weaning Mix, D : Popped Weaning Mix Mean of three replication ±SEM, Value sharing a common superscript within a control are not significant different, *p<0.05= significant difference, **p<0.01=highly significant difference, NS = Non significant difference. Table 1 shows the nutrient contents i.e. Ash, Calcium, Phosphorus, Iron, Zinc, Carbohydrate, Fat, Protein, Crude fibre and Water holding Capacity of Control and experimental weaning mix. The calcium content was found higher in the without process weaning mix and lowest in the germinated weaning mix. Phosphorus value of all experimental weaning mix was found lower compared to the Cerelac. The Iron and Zinc contents of all the experimental weaning mix were found higher compared to the Cerelac and it shows highly significant difference (p<0.01) also. The Carbohydrate was found lower in all the
  • 4. International Journal of Trend in Scientific Research and Development (IJTSRD) ISSN: 2456-6470 @ IJTSRD | Available Online @ www.ijtsrd.com | Volume – 1 | Issue – 5 Page: 187 experimental weaning mix compared to the Cerelac and they were significantly differ (p<0.01) too. Protein content was also found slightly higher compared to the Cerelac and the significant difference (p<0.05) was seen in the Malted and Popped weaning mix in comparison of Cerelac. The Water holding capacity of all the experimental weaning mix was lowest compared to the Cerelac as the Cerelac contained higher amount of carbohydrate. CONCLUSION The Calcium was higher in A sample i.e. without process weaning mix. The Phosphorous and Zinc were higher in the B sample i.e. Malted weaning mix. Whereas the Iron, Carbohydrate, Fat and Protein were higher in the D sample i.e. popped weaning mix. The Crude Fibre was found higher in the all the experimental weaning mix compared of the control sample i.e. Cerelac weaning mix. In Conclusion of the study results suggest that the weaning foods based on wheat, chickpea and ragi are nutritionally balanced and possess good growth promoting quality. The traditional technologies such as Malting, Germinating and Popping of cereals and legumes could be easily adapted to prepared weaning and supplementary foods from wheat, chickpea and ragi. Malting, Germinating and Popping have a high potential for developing low cost weaning and supplementary foods. REFRENCES  AOAC (1984), “Official Method of Analysis” 14th Edn. Associationn of official analytical chemists, Washington DC.  Clark F. P. and Collip J. B, (1925),”Determination of calcium by titremetric method”, journal of biological chemistry, 63,461.  Ezeji C. and Ojimelukwe P.C.(1993),'' Effect of fermentation on nutritional quality and functional properties of infant food formulations prepared from pambara groundnut, fruit dip pumpkin and millet seeds'', Plant Foods Hum Nutr., 44 : 267- 276.  Fiske C. H. and Subbarow (1925),“Colorimetric Determination of phosphorus”, journal of biological chemistry, 66 : 375-400.  Gandhi, A.P. and Srivastava, T. (2007), “Studies on the production of protein isolates from defatted sesame seed (Sesamum indicum) flour and their nutritional profile, Asian Food Journal, 14(3), 175-180.  Hedge, J.E. and Hefreiter, B.T. (1962), “Methods in carbohydrate chemistry”, Vol. 17 (Eds.), Whistler R.L. and BeMiller, J.N., Academic Press, New York, p.420.  Hulse J.H.(1991),''Nature, composition and utilization of grain legumes'', In: Uses of Tropical Legumes: Proceedings of a Consultants’ Meeting., 11-27. ICRISAT Center, ICRISAT, Patancheru, A.P. 502-324, India.  Jaffery G. H., Bassett J., Mendham J.(1989),”Vogel's quantitative analysis of chemicals”, Hangman Harlow.,: 5 284-294.  Kamchan A., Puwastien P., Sirichakwal P.P. and Kongkachuichai R.(2004),''In vitro calcium bioavilability of vegetables, legumes and seeds'', J. Food Composition and Analysis., 17 : 311-32.  Kjeldahl, J. (1883), New method for the determination of nitrogen in organic substances, Zeitschrift fur analytische chemie, 22(1): 366-383.  Mahajan P.V. and Chattopadhey P.K.(2000),''Development of chemically leavened cereal legume based instant mix (dhokla)'',J Food Technol., 37 : 459-464.  Negi A., Boora P. and Khetarpaul N.(2001),''Starch and protein digestibility of newly released moth bean cultivars: Effect of soaking, dehulling, germination and pressure cooking'',Nahrung., 45(4) : 251-254.  Preet K., Punia D.(2000), ''Antinutrients and digestibility (in vitro) of soaked, dehulled and germinated cowpeas'', Nutr. Health., 14(2) : 109- 117.  Ramsay W. N. M.(1969),''Method of iron estimation'', journal of biological chemistry,17, Practical clinical biochemistry – 4th edition.  Sattar A., Durrani S.K., Mahmood F., Ahnad A. and Khan I.(1989), ''Effect of soaking and germination temperatures on selected nutrients and antinutrients of mung bean'', Food Chem., 34 : 111-120.  Savelkoul F.H.M.G., Vanderpeol A.F.B. and Tamminga S.(1992),''The presence and inactivation of trypsin inhibitors, tannins, lectins and amylase inhibitors in legume seeds during germination. A review'',Plant Foods for Human Nut., 42 : 71-85.  Usha R., Lakshni M. and Ranjani M.(2010), '' Nutritional, Sensory and Physical Analysis of Pumpkin Flour Incorporated into Weaning Mix'', Mal J Nutr., 16(3) : 379-387.