Stem Cell Therapy uses the most advanced method called AAPE / MSC It's the most advanced method there is in the world, don't miss it

2.  Ideally, a stem cell for regenerative
medicinal applications should meet the
following criteria:
 Regenerative Medicine :Uses the body’s
own stem cells and growth factors is an
alternative therapeutic strategy for
repair of damage tissue, and becoming
predominant cell base therapy

3. Regenerative medicinal applications should
meet the following criteria:
 Can be found in abundant quantities
(millions to billions of cells)
 Can be harvested by a minimally
invasive procedure
 Can be differentiated along multiple cell
lineage pathways in a regulatable and
reproducible manner.
 Can be safely and effectively
transplanted to either an autologous or
allogeneic host

4.  With the increased incidence of obesity
in the Indonesia and abroad,
subcutaneous adipose tissue is
abundant and readily accessible.
 Approximately 400,000 liposuction
surgeries are performed all over the
world each year,
 These procedures yield anywhere from
100 mL to >3 L of lipoaspirate tissue

5. Hasil lemak dari
liposuction

6. pellet

7. Adipocyte after 24 hours Adipocyte after 24 hours
ADSC pasage 1 : hari ke 3

8. A B
by dr Indah Yulianto – Yuyun Rindiastuti – Dewi M

9. Courtesy by Indah Yulianto – Yuyun
Rindiastuti – Dewi M

11.  ADSCs secrete various growth factors
that repair and replace the defective
surrounding cells
 Also a good raw material for skin
regeneration, reepithelialization, wound
healing and wrinkling care instead of
ADSC itself

12. 

13. Oxygen levels & Oxygen
sensing, HIF Genetic
exogenous stability
1 response
oxidative stress
Cytogenetic
analysis, gene
expression,
bioenergetics
studies
Culture grown in -Increase genetic
physiologycal 20% instability
oxygen -Decrease glycolysis
Culture grown in -transcriptional activation
standard 3% in HIF 1A target genes
oxygen - Inhibition oxygen
consumption

14. RESULT
Growth of hMSC at 20% O2 reduces lifespan, increases
oxidative stress and the rate of telomere shortening
Cell growth
Telomerase
shortening & cell
senescence

15. Growth of hMSC at 20% O2 increases DSB generation and
chromosomal instability.
DSB level (53BP1
marker)
DNA damage &
chromosomal
aberration

16. Exogenous source
of ROS cause
growth defects &
genetic instability

18. DISCUSSION
-Promotes DNA damage &
Culture of
senescence
mammalian cells
-Chromosomal aberration during
at 20% oxygen
long term culture
Importance
of biosafety Culture ADMSC at
in cell 3% 0xygen
therapy
Increase growth rate
-No evidence of spontaneous immortalization
-- reduce telomere shortening rate
decrease structural chromosomal aberration

19. Significant upregulation of gene Balance
expression in cells grown at 3% oxygen bioenergetics
tension related to HIF 1A stabilization pattern
HIF 1A decreases
OXPHOS system by
downregulating
mitochondrial ATP
production & oxygen
consumption
Autocrine &
paracrine effect in
cell growth &
senescence
Implication of gene
ontology analysis (HIF 1
dependent gene
expression)

20. Analysis Method
Trypan blue stained cell counted
Cell number & by automatic cell counting
viability machine (invitrogen)
MSC marker CD 44, CD 106 PCR
Energy metabolism
gene related
HIF 1 A, HIF 1B,
glycolisis GLUT-1 PCR
mechanism
Pluripotency HIF 2A, Oct-4, Sox-
marker 2, Rex-1 PCR
Courtesy by Yuyun Rindiastuti

21. RESULT
Cell proliferation &
viability
5,000,000
4,500,000
4,000,000
3,500,000
Cell number
3,000,000
2,500,000
2,000,000
1,500,000
1,000,000
500,000
0
Graph 1. Cell number
profile

22. 98%
96%
94%
92%
Cell viability
90%
88%
86%
Graph 2. Cell viability
Courtesy By Yuyun RA

23. MSC surface marker
expression

24. HIF 1A & GLUT 1 expression
Normoxic group Hypoxic group
Courtesy By Yuyun RA

25. 3 days after skin needling + MCSs + product

26. Acne and Acne Scar 1 week After Skin Needling + MCSc +
Product

28. Acne and Acne Scar 1 week After Skin Needling +
MCSs + Product
Courtesy by Titik Moertolo

30. By Indah Yulianto

32. By Indah Yulianto

34. By Indah Yulianto

35. 1
2
Courtesy by dr Titik Moertolo

37. 3
4

38. Courtesy by dr Titik Moertolo

39. Acne Scar & Box scar 1 week post Dermaroller

40. 2 weeks after Skin Needling +
Acne Scar before (Box Scar)
MShCs
Courtesy by dr Titik Moertolo

42. BY Indah Yulianto

44. 1 month after Mesenchymal product (
Injections and Cream) – Men 74 years
old
By Indah Yulianto

45. 3 weeks after MSCs
Product Injections +
Cream
BY Indah Yulianto

46. Bone 1 week after
2 weeks after (painful & sensitivity +)
25 % reduce in 2 weeks

47. Luka bakar + infeksi 5 bulan intra kutan produk MShCs
y.l.
regenerasi
Hari ke 15 : penyembuhan 75% tanpa
Pengolesan Krem + Produk MShCs Cicatriks ataupun Retraksi persendian