PRP is a breakthrough technique on skin care & treatment to preserve it's beauty & health at a very affordable cost and higher satisfactory result compared to other methods.

1. NEW EVOLUTION OF AESTHETIC BIOTECHNOLOGYSPECIALIST IN NONABLATIVE SKIN REJUVENATION AND AUTOLOGOUS CELLULAR REGENERATION Indah Yulianto.,Yulianto D., EndraYustin., Ari Kusumawardhani. DERMATO VENEREOLOGY DEPARTMENT MEDICAL FACULTY OF SEBELAS MARET UNIVERSITY / DR. MOEWARDI GENERAL HOSPITAL SURAKARTA

2. Skin treatment action levelsMultidisciplinary Program For the Rejuvenation for the face FILLERS Dermocoemeticis Peels Wrinkles & Volumes correction Renewal of the corneal layer BIO Stimulation PRP Germinative layer stimulation Dermis stimulation Architectural skin reconstruction/reorganization

3. What is BEAUTY? Beauty is a balance between form and volume Beauty produces in us an aesthetic feeling, an admiration by pleasing the eye Beauty does not captivate through detail but through the whole,which is greater than the sum of the individual parts Beauty does not only have to do with the face, the voice, the body or a charming appearance.

4. Platelet-Rich Plasma (PRP) PRP has been breakthrough in the stimulation and acceleration of soft tissue healing, rejuevenation It represents a relatively new biotechnology that is part of the growing interest in tissue engineering and cellular therapy today The clinically useful product is a concentration of platelets in small volume plasma and is therefore a “platelet-rich-plasma”

5. It come drop by drop from our blood Anatomy and Fungtion from platelets

6. PRP – harvesting : Autologous Whole Blood Rpm/minutes Platelet poor plasma and Buffy coat Erythrocyt Rpm/minutes The patient’s blood is drawn Platelets Poor Plasma Platelets Rich Plasma Steps in the preparation of platelet-rich plasma. Platelet-rich plasma (in the syringe) and platelet-poor plasma (in the centrifugation tube). The latter can be used to cover the surgical area.

7. Hypothesis If platelets concentrate could be brought to level greater than eight times baseline the physiological effects may be profoundly positive. (Dr Astley E Smith.,GlobalAutografting Institute)

8. PRP AND HC PRP

9. FIELDS APPLICATION of Platelets Rich Plasma

10. Some Growth Factors Acting on “Healing Cascade” In addition the activated thrombocytes have onto their surface a multitude of signalisation molecules eg. CD9, CD-W17, CD31, CD41, CD42a-d, CD51, CD-W60, CD61, CD62P, CD63

11. Advantages of a-Plasma Tissue regeneration & rejuvenation: neo-collagenesis(TGFα& β), neo-vascularisation(EGF & VEGF), & extracellularmatrix formation (PDGFαα& ββ& αβ) –NB: growth factors in genetically pre-determined ratio! Bio-glue (fibrin glue): haemostasis & tissue adhesion in skin flaps, bone grafts, trauma intra-surgery and post-surgery Safety: non-allergenic & free from concerns over transmissible diseases e.g. HIV, Hepatitis B & C, CJD, etc. Autologous: no risk of rejection reaction Wound healing time: increased Physiological ‘anti-biotic’: anti-bodies & WBC’s& proteolyticenzymes Plasma includes: hormones, biotransformedvitamins & other nutrients Tissue engineering: in-vitro autologous tissue culture-medium. Ease of use: dermal & hypodermal injections Convenience: Plasma harvesting performed in doctor’s rooms (no external laboratory required)

13. Contra -Indications Platelet Dysfunction Syndrome Critical Thrombocytopenia Hypofibrinogenaemia HaemodynamicInstability Auto-immune disease Malignancy Sepsis Acute & Chronic Infections Chronic Liver Pathology Anti-coagulation therapy Pregnancy (for cosmetic indications)

14. BEFORE 1 Month After

15. AFTER – 3 months BEFORE

16. A – 64 years – old woman, injection by autologous PRP 1 month after.

17. After – scratching by rat – 3 weeks ago Acne Scar : Subcision + hc PRP : 1 month after

18. 4 days after injections before

19. Acne Scar : Dermaroller 1.5 mm + PRP Before 1 week after (right side)

20. Acne Scar + Subsicion + PRP 1 week after Subsicion with Nocor needle Before

21. 3 weeks after Alopecia – 36 years old

22. Alopecia and Marking 3 weeks after Injeksi PRP In Bulbus

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