A difference between bacterial and eukaryotic transcription Multiple Choice RNA is synthesized 5’ to 3’ in bacteria, but 3’ to 5’ in eukaryotes. in eukaryotes, there are different RNA polymerases (I, II, and III). in bacteria, introns have to be removed from the primary RNA transcript. transfer RNAs (tRNAs) are involved in eukaryotic transcription only. in bacteria, the start signal is an AUG, while in eukaryotes it is a promoter. Solution Differences. there are different RNA polymerases (I, II, and III). in bacteria, introns have to be removed from the primary RNA transcript hope it helps ....Regards.