2. Polarizing Light Microscopy
• It is a technique where polarized light is used
in microscope to evaluate composition and
three dimensional structure of anisotropic
specimen.
5. • Normal light vibrate in many plane or
vibration direction.
• In polarized microscope, a polarizer is used
which allow passage of light of one vibration
direction.
10. • Polarizer and analyzer (which is also a
polarizer) are placed at right angle.
• So, normally no light will pass through and the
viewer will only see darkness.
14. Birefringence
• Birefringence is defined as the double
refraction of light due to differences in
refractive index observed in anisotropic
substance.
• Here, incident ray of light is split into two rays,
called an slow ray and an fast ray.
15.
16.
17. • When the waves emerge from the crystal they
will be polarized at 90o to each other.
• But, when object is at 45°, the phase
difference between the two rays which can
develop are able co combine in the analyzer
and form a visible image- interferance.
19. How to use.
Analyzer is pushed completely in place.
Polarizer is placed and is rotated until the
field of view become dark.
Specimen is placed on the stage and polarizer
is rotated to evaluate the specimen.
Light reflect off the objects and then enter in our eye. Then we see the object.
Gouty deposits may be surrounded by fibrous tissue and be rimmed by histiocytes and giant cells … histiocyte…. gout crystals are long, slender, needle shaped but difficult to visualize with routine staining because they are dissolved during formalin processing