Which of the following dysfunctions in the Na+/K+ ATPase will have the most dramatic effect on the establishment of a concentration gradient? A) A reduction of the rate of ATP hydrolysis B) An increase in the K+ binding affinity in the phosphate bound form B) A decrease in the Na+ binding affinity to the phosphate bound form Solution All the dysfunctions mentioned below have the effect on the concentration gradient. But mainly it is caused due to the redutcion in rate of ATP hydrolysis. Because after binding of the Sodium ions, the ATP has to undergo hydrolysis into ADP .The Sodium ions loose affinity and are released and increased affinity of K+ ions for ADP increases dephosphraltion and movement of K+ ions into cell. -So intially the soidum ions should have greater affinity to ATP - ATP hydolysis should occur - Na+ ions should loose affinity to ADP - K+ ions should have greater affinity to ADP - ATP formed should be able to release K+ ions inside and revert back and atract Na+ ions..