CRYSTALLIZATION AND CRYSTALS -ABINAYA

1. SUB: APPLIED BIO CHEMISTRY
ABINAYA.P

2.  CRYSTALLOGRAPHY - It is simply a funcy
word meaning “ THE STUDY OF CRYSTALS”
 The study of crystalline soilds and the
principle that govern their growth, external
shape, and internal structure.
 Crystallography is easily divided into 3
sections geometrical, physical, and chemical.

3.  X-ray crystallography uses the uniformity of
light diffraction of crystals to determine the
structure of a molecule or atom.
 Then they use an X-ray beam to “HIT” the
crystallized molecules.
 The electrons surrounding the molecules
diffract as the X-rays hit them.
 This forms a pattern, this types of pattern is
called the X-ray diffraction pattern.

4.  Classification of crystals according to the
degree of crystallization.
1. EUHEDRAL CRYSTALS
2. SUBHEDRAL
3.ANHEDRAL CRYSTALS

6.  Crystal Properties, Space groups
 Diffraction
 Bragg’s Law, von Laue condition
 X-RAY diffraction data collection

7.  OBTAINING PROTEIN;
[e.g. Molecular Cloning and Expression]
 PROTEIN PURIFICATION;
[e.g. Fast protein liquid
Chromatography]
 PROTEIN CRYSTALLIZATION;
[e.g. Vapor diffusion technique]
 DATA COLLECTION;
[e.g. X-rays diffraction experiment]
 STRUCTURE DETERMINATION;
[e.g. Phasing, model building and
refinement]

8. THE END