Mitochondrial enzyme- cytochrome c oxidase activity lab1. What are.pdf

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arri2009av

Max Points: 5.0 A researcher is examining preferences among four new flavors of ice cream. A sample of n = 80 people is obtained. Each person tastes all four flavors and then picks a favorite. The distribution of preferences is as follows. Do these data indicate any significance preferences among the four flavors? Test at the .05 level of significance. Which test is used and why?? Ice Cream Flavor A B C D 12 18 28 22 Ice Cream Flavor A B C D 12 18 28 22 Solution no.all the values are too low to be of any significant value at 0.05 level of significance.

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Mitochondrial enzyme- cytochrome c oxidase activity lab
1. What are the four main components of complex 4 or cytochrome c oxidase?
2. What happened to the absorption 55nm after addition of mitochondrial preparation and why?
Solution
Answer:
1) The enzyme cytochrome c oxidase (or called as complex IV) is a large protein complex
located in the membrane and found in mitochondrion of eukaryotes and in membrane of bacteria
as well. It is an essential enzyme due to its regulatory role in the respiratory electron transport
chain of mitochondria (or bacteria). It is the last enzyme which is located in the mitochondrial
(or bacterial) membrane thus, catalyze the last step of the respiratory electron transport chain
(oxidative phosphorylation).
It is a large protein, consists of 14 protein subunits and several metal prosthetic sites however,
the four main components of Complex IV are:
The function of the enzyme complex IV involve to receives an electron from each of four
cytochrome c molecules, and then transfers those electrons to one oxygen molecule. Thus, the
molecular oxygen reduced to two molecules of water and ATP synthesized during the reaction.
As during the catalysis, the enzyme binds to four protons from the inner aqueous phase of
mitochondria (to make water). And four protons are translocated across the membrane which
results to a transmembrane difference of proton electrochemical potential. And this proton
electrochemical potential is than used by ATP synthase to synthesize the ATP.

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