Why is the answer E. coli B? Bender\'s Experiments Mutations rll1 and rI12 are known to fall
into the rlLA and rllB citrons (genes) respectively. Mutations rll1 and rIL2 are used to infect E.
Coli K at high multiplicity of infection (m. o. i.) and the progeny phage collected. These phage
are then used to infect strain B at high m. o. i. The resulting phage are diluted and plated on both
E. Coli B and E. Coli K. Do you expect more plaques on strain B, on Strain K, an equal amount
on both or is it impossible to tell? (circle one) E. coli B E. Coli K Equal on both Can\'t tell
Solution
E. coli B strain allows both mutant as well as wild type phage’s to grow on it hence more number
of plaques is noticed on it, whereas E. coli K allows only wild type recombinant phage’s to grow
on it hence it produces lesser number of plaques than compared to E. coli B strain..
Why is the answer E. coli B Benders Experiments Mutations rll1 an.pdf
1. Why is the answer E. coli B? Bender's Experiments Mutations rll1 and rI12 are known to fall
into the rlLA and rllB citrons (genes) respectively. Mutations rll1 and rIL2 are used to infect E.
Coli K at high multiplicity of infection (m. o. i.) and the progeny phage collected. These phage
are then used to infect strain B at high m. o. i. The resulting phage are diluted and plated on both
E. Coli B and E. Coli K. Do you expect more plaques on strain B, on Strain K, an equal amount
on both or is it impossible to tell? (circle one) E. coli B E. Coli K Equal on both Can't tell
Solution
E. coli B strain allows both mutant as well as wild type phage’s to grow on it hence more number
of plaques is noticed on it, whereas E. coli K allows only wild type recombinant phage’s to grow
on it hence it produces lesser number of plaques than compared to E. coli B strain.
