1. The osmolarity of a red blood cell is 300 mOsM. Are the solutions.pdf

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1. The osmolarity of a red blood cell is 300 mOsM. Are the solutions used iso-osmotic, hyperosmotic, or hypo-osmotic to the red blood cells? 2. Why then did some of the solutions cause red blood cell lysis (think in terms of permeant vs impermeant solutes and tonicity)? Solution 1. ANS: If the osmolarity of a red blood cell is 300 mOsM. Then the solutions is used for iso- osmotic medium to the red blood cells. The hyper-osmotic had 600 mOsM osmolarity and the hypo-osmotic solution had 100 mOsM osmolarity. 2. ANS: Permeant solute can move freely across semipermeable membrane. Impermeant solutes cannot cross semipermeable membrane. Red blood cell lysis: 1. A cell in hypotonic solution: It gains water. Cell Ruptures (Due to the Hemolysis of RBC). 2. A cell in hypertonic solution: Cell losses water. Cell shrinks (Due to the Crenation of RBC)..

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5. Cells with an intracellular osmolarity of 300mosm/L are suspended in the following solutions. Describe the sequence of events that take place. (a)0.3M urea (membrane permeable) (b)0.3M glucose (membrane impermeable) (c)0.6M urea (d)0.6M glucose Solution Osmolarity of a solution refers to the concentration of the solution in terms of how strongly it attracts water molecules across a semipermeable membrane. It is given the intracellular osmolarity of the cell is 300 mosm/L. It can also be expressed as 0.3 osm/L or 0.3 M. (a) 0.3 M Urea (membrane permeable) This solution is isosmotic to the intracellular osmotic pressure. But, the cell membrane being selectively permeable, allows the entry of urea until both the solutions become isotonic. This leads to bursting ofcell as along with urea water enters the cell. (b) 0.3 M Glucose (membrane impermeable) This solution is also isosmotic to the intracellular osmotic pressure. But given that the membrane is impermeable, glucose cannot enter to cell and an isotonic condition cannot be reached. Hence the cell would be unstable and would distort due to the uneven tonicity. (c) 0.6 M urea This solution is hypertonic and hyperosmotic as compared to the cell\'s intracellular fluid. Thus, water will move out through the pores of the cell membrane causing the cell to shrink or become flaccid. (d) 0.6 M glucose This solution is hypertonic and hyperosmotic as compared to the cell\'s intracellular fluid. Thus, water will move out through the pores of the cell membrane causing the cell to shrink or become flaccid..

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