DNA Paternity Testing Simulation Post-lab Questions A)Why do different individuals such as siblings have different restriction enzyme recognition sites? B) What is the function of probes in DNA praternity analysis? C) Why is there more than one single locus probe used in an actual paternity DNA test? D)Why do we not use probes in this DNA paternity simulation and still obtain results? Solution A)Why do different individuals such as siblings have different restriction enzyme recognition sites? The different individuals such as siblings have different restriction enzyme recognition because the size of the gene fragments are mainly relies upon the “distance between the recognition sites”. This is due to polymorphism i.e. single nucleotide polymorphism (SNPs) with intervening repetitive base sequences in siblings. The paternal parents are goiung to inherit genetic variability into the sibling due to gametes reduction division & crossing over B) What is the function of probes in DNA praternity analysis? The functions of DNA probes are meticulously to identify “length differences between these intervening repetitive gene sequences via isotopic & non-isotopic labeling by the use of “single stranded DNA finally these DNA probes are going to complement and hybridize (attach) to single stranded DNA due to SNP during paternity analysis. C) Why is there more than one single locus probe used in an actual paternity DNA test? More than single locus DNA probe or Multiple- Locus probes are used to identify “multiple intervening repetitive DNA sequences” located on many chromosomes result in generation of 20 to 30 bands so that is possible to avoid chances of same pattern due to the multi-band patterns,.