You are given a mixed culture containing a anaerobic thermophile, aerobic thermophile and anaerobic hyperthermophile bacteria. How will you separate these into pure cultures? Solution In natural environments, microorganisms usually exist as mixed populations. A pure culture is one by which all organisms are descendants of the exact same organism. A sterile medium is the one which is free of all life forms. It is often sterilized by heating it to a temperature at which all contaminating microorganisms are destroyed. Eventually, in functioning with microorganisms, we ought to have a way of transferring growing organisms from a pure culture to a sterile medium without introducing any undesirable outside contaminants. This approach of stopping unwanted microorganisms from developing accessibility is termed aseptic technique. Microorganisms whose optimal temperature for development is near 0oC are called psychrophiles. The ones that grow optimally between 20oC and 45eC are called mesophiles. These growing most useful at large conditions, 55oC to ~ 70oC and larger, are called thermophiles. Prokaryotes that live at quite high conditions (up to 113oC ) are hyperthermophiles. An organism that will require air for development is called aerobic. An organism whose development can\'t arise in the presence of air is anaerobic. An organism that may grow under either aerobic or anaerobic conditions is a facultative anaerobe. Microaerophilic organisms involve free air but at a limited concentration. Based on this, the plates are incubated at different temperatures with aerobic and anerobic conditions for enhancing the growth of thermophiles and hyperthermophilc bacteria..

1. You are given a mixed culture containing a anaerobic thermophile, aerobic thermophile and
anaerobic hyperthermophile bacteria. How will you separate these into pure cultures?
Solution
In natural environments, microorganisms usually exist as mixed populations. A pure culture is
one by which all organisms are descendants of the exact same organism. A sterile medium is the
one which is free of all life forms. It is often sterilized by heating it to a temperature at which all
contaminating microorganisms are destroyed. Eventually, in functioning with microorganisms,
we ought to have a way of transferring growing organisms from a pure culture to a sterile
medium without introducing any undesirable outside contaminants. This approach of stopping
unwanted microorganisms from developing accessibility is termed aseptic technique.
Microorganisms whose optimal temperature for development is near 0oC are called
psychrophiles. The ones that grow optimally between 20oC and 45eC are called mesophiles.
These growing most useful at large conditions, 55oC to ~ 70oC and larger, are called
thermophiles. Prokaryotes that live at quite high conditions (up to 113oC ) are
hyperthermophiles. An organism that will require air for development is called aerobic. An
organism whose development can't arise in the presence of air is anaerobic. An organism that
may grow under either aerobic or anaerobic conditions is a facultative anaerobe. Microaerophilic
organisms involve free air but at a limited concentration. Based on this, the plates are incubated
at different temperatures with aerobic and anerobic conditions for enhancing the growth of
thermophiles and hyperthermophilc bacteria.