These are the slides of my part in the "Why the Fly?" event at the Tiedekulma of the University of Helsinki on October 11, 2016. You can listen to the talk at: https://www.helsinki.fi/fi/unitube/video/98eba1ad-fdf9-44de-ae10-105b8b7cc9c5
2. Creation of
mutant flies
12h dark-light cycle
until late
metamorphosis
Transfer to
complete dark
Finding the
mutant genes
Count adult flies
per hour
for each mutant
Comparison
of mutants
Konopka and Benzer, 1971
3. Creation of
mutant flies
12h dark-light cycle
until late
metamorphosis
Transfer to
complete dark
Finding the
mutant genes
Count adult flies
per hour
for each mutant
Comparison
of mutants
Manchester Fly Facility
4. Creation of
mutant flies
12h dark-light cycle
until late
metamorphosis
Transfer to
complete dark
Finding the
mutant genes
Count adult flies
per hour
for each mutant
Comparison
of mutants
Konopka and Benzer, 1971
24h
5. Creation of
mutant flies
12h dark-light cycle
until late
metamorphosis
Transfer to
complete dark
Finding the
mutant genes
Count adult flies
per hour
for each mutant
Comparison
of mutants
Konopka and Benzer, 1971
19h
28h
24h
6. Creation of
mutant flies
12h dark-light cycle
until late
metamorphosis
Transfer to
complete dark
Finding the
mutant genes
Count adult flies
per hour
for each mutant
Comparison
of mutants
Konopka and Benzer, 1971
OMIM
7.
8. Lily Yeh Jan &
Yuh Nung Jan
Nature
Howard Hughes Medical Institute
Seymour Benzer
Physics: Transistors
Molecular genetics: Divisibility of the gene
Mid-60s: Behavior:
His two daughters having different personalities
Himself being an owl and wife being a lark
Chooses Drosophila for behavioral studies
All these were mapped onto the same gene by
Physical mapping on the polytene chromosome
Explain polytene chromosomes
Explain deficiency (=deletion)
(2) Recombination frequency with other known genes like yellow, scute, white
All these were mapped onto the same gene by
Physical mapping on the polytene chromosome
Explain polytene chromosomes
Explain deficiency (=deletion)
(2) Recombination frequency with other known genes like yellow, scute, white
All these were mapped onto the same gene by
Physical mapping on the polytene chromosome
Explain polytene chromosomes
Explain deficiency (=deletion)
(2) Recombination frequency with other known genes like yellow, scute, white
All these were mapped onto the same gene by
Physical mapping on the polytene chromosome
Explain polytene chromosomes
Explain deficiency (=deletion)
(2) Recombination frequency with other known genes like yellow, scute, white
All these were mapped onto the same gene by
Physical mapping on the polytene chromosome
Explain polytene chromosomes
Explain deficiency (=deletion)
(2) Recombination frequency with other known genes like yellow, scute, white
This is a 3rd instar larva.
Discovered by the Jans, who were Benzer’s postdocs
Neurons are lying under the epidermis in 2D.
Each abdominal segment has the same neurons, with the same shape.
Class I neurons: Simple arbor, Abrupt+
Class IV neurons: Complicated arbor, Abrupt−
First: Orientation
Well studies regions like the MB
Some regions with no name until 2014
Some regions redrawn and renamed due to better understanding of the anatomy & embryolgy
3 layer cateories
