lab 2

1. Amounts of proteins with corrected absorbance at 0.046,
0.035 and 0.007

2. corrected absorbance = 0.007
corrected absorbance = 0.035
corrected absorbance = 0.046
mgmgmgmg 665.455.2785.133.0 
4.665 mg in 50 ml
Therefore, the amount of protein in eluted fractions is 4.665mg and hence the original protein
concentration of the BSA-cobalt sample is 93.3 mg/ml.
mgml
ml
mg
55.2300
200
70.1

mgml
ml
mg
33.0300
200
22.0

mgml
ml
mg
785.1300
200
19.1

mlmg /3.93665.4
50
1000
