Prac A (4) Question 5

1. We use Beer – Lambert Law
The activity of an enzyme is expressed as Units,
where one Unit is the amount of enzyme that
converts 1 μmol of substrate to product per minute
under defined conditions. This represents the
velocity (ν). We need to convert the unit into 3ml
because it is the total volume of NaP buffer, NADH,
pyruvate and LDH used in 10ml yellow cap plastic
tube.

2. For example : Reaction 1a