Pathological study of blood parasites in rice field frogs

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OUTLINE
 Introduction
 Methods
 Results
 The prevalence of blood parasites
 Lesions
 Conclusions
 References

Rice Field Frog, Hoplobatrachus rugulosus

INTRODUCTION
 In Thailand, one hundred and forty rice
field frogs were collected for blood
parasite examination.
 Researches in several regions have
indicated that frogs have infected with
variety of blood parasites.
 The frogs are important because they
are one of the main food sources of
the local population.

METHODS
 Researchers took blood samples from the
frogs.
 The blood was preserved in heparinized
tubes and examined for hematocrit value.
 The blood drops were smeared and fixed
with methanol.
 The parasitemia was calculated by
counting the number of parasites per
5000 eryrthrocytes on a thin blood film.

RESULTS

 70% of the frogs were positive for
blood parasites.
 The frogs visceral organs were
infected with just one spices of blood
parasite.
 It was identified that the main vectors
are leeches and mosquitoes.

THE PREVALENCE OF BLOOD
PARASITES
Blood parasite Prevalence (%)
(+ve/total frogs)
Trypanosoma rotatorium- 11.4% (16/140)
like organism
Trypanosoma chattoni 22.2% (31/140)
Hepatozoon sp. a 4.3% (6/140)
Hepatozoon sp. b 1.4% (2/140)
Lankesterella minima 7.1% (10/140)

LESIONS
 Infected with Trypanosoma rotatorium and
Trypanosoma chattoni – no pathologic
lesions in the visceral organs tissues.
 Infected with Lankesterella minima -
lesions in liver, lung, spleen and kidney.
 Infected with Hepatozoon sp.a and b -
pathologic lesions in liver.

CONCLUSIONS
 To sum up, there was no statistical
difference between the hematocrit value of
infected and noninfected frogs.
 The frogs visceral organs were infected
with just one spices of blood parasite.
 The infected frogs did not show any clinical
signs of sickness.
 The present study importance lies in the
ecological conservation.

REFERENCES
„Pathological Study of Blood Parasites in Rice
Field Frogs, Hoplobatrachus rugulosus
( Wiegmann, 1834)“
Achariya Sailasuta, Jetjun Satetasit, Malinee
Chutmongkonkul
Veterinary Medicine International, 2011

http://www.hindawi.com/journals/vmi/2011/850
568/

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