1. BIOHELLENIKA SERVICES

3. PLATELET RICH PLASMA
(PRP)
1.
Platelet Rich Plasma (PRP) is an autologous advanced ŸMinimal discomfort: reduces the pain duration much
skin rejuvenation product derived from patient's own faster
blood as a serum enriched with growth factors from
ŸShorten downtime: faster stimulation of healingplatelets and bioactive proteins that induce cell growth
processand regeneration. This personalized serum aims to
stimulate collagen and elastin production of the skin
ŸCost Effective: the cost is lower compared to
fibroblasts, both molecules fill the desired areas. After a
alternative treatments
specific process, patient's blood results to a PRP serum, a
natural anti-aging solution very beneficial to skin ŸSimple procedure
rejuvenation. Also, PRP eliminates acne scars and
ŸLong lasting result: rejuvenation lasts for a longerminimize skin pores leaving your skin smoother and
period of time comparing with other fillersyounger.
ŸNot causes traumaPlatelet Rich Plasma is an increasingly popular method
as it is the ideal collagen and elastin stimulating therapy
for treating ageing skin of face, neck, eyes, hands and
décolletage by improving texture, tone, fine lines and
wrinkles, helping it to become progressively thicker,
tighter and suppler.
Benefits of PRP Therapy
PRP method has many advantages such as:
ŸNatural result without the risk of 'looking done' or
asymmetry
ŸNo risk of transmissible infection, rejection, allergic
reaction or allergy (100% your own blood by-product)
PRP
AESTHETICS
VETERINARY PLASTIC
SURGERY
ORTHOPEDICS
DENTISTRY
OPHTHALMOLOGY

4. PLATELET RICH PLASMA
(PRP)
PRP APPLICATIONS – CLINICAL AREAS
Today Platelet Rich Plasma is used to:
Manage your skin age…
a radiant and fresh look just for you
Ÿrejuvenate facial skin (including nasolabial lines,
marionette lines, forehead lines), neck – chest –
décolletage aging
Ÿimprove the texture of the skin, fine lines, wrinkles,
especially around and under the eyes and neck
Ÿimprove the quality of aging skin
Ÿcorrect acne or other scars
Ÿpost skin laser treatment
Ÿimprove stretch mark
Ÿback of hands and arms reduce hair loss, strengthen
hair roots and nourish hair follicles
AESTHETICS
Hair loss
Fine lines and wrinkles
around and under the eyes
Wound and scar healing
Cheeks and entire face
Hand and arms
Jaw
Neck
Chest and décolletage
Loose skin & skin tone
Hand and arms
Other body areas

5. PLASTIC SURGERY
Platelet Rich Plasma stimulates angiogenesis, promoting
vascular growth and fibroblast proliferation. In addition,
PRP functions as haemostatic by forming a fibrin clot.
Also application of PRP enhances wound-healing in both
soft and hard tissue.
Biohellenika has developed autologous therapeutic
products for the healing of:
Ÿpressure sores
Ÿdiabetic ulcers
Ÿburns
Ÿwounds

6. PLATELET RICH PLASMA
(PRP)
ORTHOPEDICS
The earlier treatment, the better outcome
Except the natural process of ageing, our life itself
Platelet Rich Plasma (PRP) is a 100% non surgical naturalincludes injuries, traumas and pain. PRP is very effective
treatment for pain and injury. PRP is injected directly toin treating osteoarthritis, especially in early stages. PRP
the site of injury trigging body's nature to repair itself in astimulates cartilage regeneration inhibiting the
drug-free way within a short recovery time for mostdeterioration effects of age and slows the development
patients.of arthritis.
Biohellenika owns innovative technology and products to
enhance soft and bone tissue healing.
Most common applications
Ÿcartilage/ligament repair
Ÿosteoarthritis
Ÿtendonitis
Ÿpost-operative pain
Ÿinflammation reduction
Ÿchronic pain
Natural Therapy, No Side Effects
Avoid Major Surgery! Heal More Rapidly

7. DENTISTRY
Platelet rich plasma enhances bone regeneration. PRP applications were first developed in veterinary
Biohellenika is one of the pioneers that developed a new medicine and now is used for
approach to apply PRP
Ÿtendon and ligament injuries
Ÿbone graft for closure clefts, lips, fistulas and palate
Ÿwound healing
defects
Ÿosteoarthritis
Ÿrepair bone defects after tooth or cysts extraction
Ÿimplant in the dental surgery
1. Treatment With Platelet-Rich Plasma Is More Effective Than Placebo
for Knee Osteoarthritis: A Prospective, Double-Blind, Randomized
Trial.
Patel S, Dhillon MS, Aggarwal S, Marwaha N, Jain A.Am J Sports Med.
2013.
PRP's growth factors quicken the recovery of corneal 2. A Novel Method For Iatrogenic Vesicovaginal Fistula Treatment:
Autologous Platelet-Rich-Plasma Injection And Platelet-Rich-Fibrin-epithelial cells, shorten the recovery of surgical area by
Glue Interposition.
carrying out anti-inflammatory activity, minimize post-
Shirvan MK, Alamdari DH, Ghorifi A. J Urol. 2012 Dec 28surgery pain and stabilize the corrected eyesight by
stimulated healing.
Ÿepithelial defects
Ÿtrauma of the cornea
Ÿdry eye syndrome
Ÿpost laser surgery syndrome
OPTHALMOLOGY
VETERINARY

8. 2.
AUTOLOGOUS CONDITIONED SERUM
(ACS)
Today's applications show that its most common uses are phalangeal joints, knee, shoulder, muscular injury in
in osteoarthritis, especially in the knee and the hip joint athletes and the sciatica and lumbar back pain.
area. As people mature, the percentage of the population
Although osteoarthritis can't be cured yet, symptoms canthat suffers from osteoarthritis increases. A special
be treated effectively and degenerative process will bemessenger, the protein interleukin-1 (IL-1) plays an
delayed. The Autologous Condition Serum enhancesessential role in the development of osteoarthritis. It
body's natural defense mechanisms againstpromotes joint and cartilage inflammation that leads to
inflammation, reduces pain, improves function andpain.
promotes natural healing. A safe, simple and effective
This protective protein called interleukin-1 receptor treatment.
antagonist (IL-1Ra) suppresses the aggressive IL-1 and
thus acts anti-inflammatory, analgesic and cartilage- ConcernsAllofUsforaNewLifetime
protective.
Except the natural aging process, other factors such as
overweight, genetic predisposition, injuries lead to pain,
severity of osteoarthritis and loss of mobility.
Beneficiary Effect on Osteoarthritis
A unique natural medical procedure in which an
autologous blood product is introduced to the patient's
body to reduce chronic pain, osteoarthritis and treat
arthritis of the elbow, antebrachiocarpal joints, tarsi,

9. applications. The younger age the fibroblasts, the betterDiscover YOUTH a revolutionary personalized cell
results. So, we advise that fibroblasts should beproduct using your own fibroblasts for face rejuvenation
preserved in young ages. Fibroblasts injection usually is
Fibroblasts, the richest source of collagen and elastin, are
combined with PRP for even better results.
widely spread cells in the body, especially in dermis. Aging
decreases all biological activities of fibroblasts like cell
proliferation and secretion of extracellular matrix
components, thus the skin ages.
YOUTH, composed by pure autologous fibroblasts,
improves the appearance of your skin. Its implantation
enriches the skin with activated cells that produce
collagen and elastin for long term rejuvenation of the
skin. Fibroblasts are extracted from a small skin biopsy,
cultured in Biohellenika's laboratories to reach an
adequate number, activated by a low energy laser and
injected into the wrinkles and the imperfections of the
face.
Effective and Simple
YOUTH is prepared especially for you!!! The method is
safe without any risk, no complications but with long term
results. The fibroblasts can be cryopreserved in
separated vials and used in more than once future
3.
KEEP YOR JUVENILENESS
FOR EVER

11. BenefitsMy Cream is a sur mesure revolutionary anti-aging
product based on the latest discoveries in stem cell
ŸFirmer and smoother skin
research. This advanced formula completely restores
ŸReduction of fine lines and wrinklesskin's youth, diminishes wrinkles, reverses skin damage
and minimizes visible signs of aging. ŸPlumper skin
My cream contains high-tech ingredients using the self- ŸDeeply hydrated skin
renew abilities of stem cells promoting firmness and
elasticity leaving skin smoother and younger-looking.
ApplicationMy Cream, since autologous, is personalized for each
individual according to the skin type and age. Apply My cream twice a day (morning & evening) after
cleansing. Gently massage in until the cream is absorbed.
Size: 30ml
Two Products To Try
Two types of My Cream are available containing a
unique anti-aging cream base enriched with two different
skincare extracts supporting skin's natural repair process.
For the first type, the base is mixed with autologous PRP
and for the second we mix the supernatant of the
autologous mesenchymal stem cell cultures and the
cream base. Both types help cell renewal, have incredible
nourishing abilities and prevent the skin from aging
prematurely.
4.
MY CREAM

12. 1. Mosna F, Sensebé L, Krampera M.Mesenchymal stem cells are collected from adipose
Human bone marrow and adipose tissue mesenchymal stem cells: a
tissue after liposuction and are used for cosmetic and
user's guide.
regenerative medicine applications. Adipose tissue Stem Cells Dev. 2010 Oct;19(10):1449-70.
contains a fraction of stem cells, 1.000 times more than 2. Rada T, Reis RL, Gomes ME.
Adipose tissue-derived stem cells and their application in bone andother source. The fraction contains perivascular
cartilage tissue engineering.
mesenchymal stem cells from the vessels of
TissueEng Part B Rev. 2009 Jun;15(2):113-25.
microcirculation, vascular endothelial and proendothelial 3. Zhen G, Liu H, Gu N, Zhang H, Xu Y, Zhang Z.
stem cells and mature fibroblasts. In cosmetic medicine, Mesenchymal stem cells transplantation protects against rat
pulmonary emphysema.
mesenchymal cells are used for face reconstruction
Front Biosci. 2008 May 1;13:3415-22.
combined with PRP, to treat wrinkles and skin
4. Griffin M, Iqbal SA, Bayat A.
rejuvenation. Also, mesenchymal cells are used to treat Exploring the application of mesenchymal stem cells in bone repair
and regeneration.autoimmune diseases, like multiple sclerosis, Crohn's
J Bone Joint Surg Br. 2011 Apr;93(4):427-34.
disease, ulcerative colitis, rheumatoid arthritis and newly
diagnosed diabetes type I. Also are used to treat chronic
pulmonary obstruction, osteoarthritis (traumatic or
degenerated), fractures, diabetic ulcers and burns.
5.
MESENCHYMAL STEM CELLS
FOR COSMETICS OR REGENERATIVE MEDICINE

13. Clinical applications: 3. Autologous chondrocytes implantation. This
method is applied to young patients in the cases of
1. Cartilage rejuvenation (simple incidents). sports injury and not to elder patients, because the
Mesenchymal stem cells modification to ability of chondrocytes to proliferate is decreased
chondrocytes. Biohellenika has developed an during the age. A small biopsy of the healthy part of
innovative technology to isolate, purify and regenerate the cartilage is removed and the chondrocytes are
the patient's own chondroblasts and chondrocytes for separated after enzymatic digestion. The
either intrarticular or arthroscopic re-injection. In the chondrocytes are proliferated and since an adequate
case of osteoarthritis at any age, mesenchymal cells number of cells, according to the extent of injury,is
extracted from adipose tissue modified to accomplished they are implanted into the damaged
chondrocytes and with minimal invasive endoscopy area, in a three dimensional sticky gel of fibrin glue.
applied into the damaged cartilage. The number of This method is applied in the cases of cartilage loss of
2injected cells is analogous to the extent of the damage. over 8cm . The cartilage reconstruction is applied in all
genders, age 15-60 years old, for damaged areas
2
ranging from 1.6 to 12 cm and in athletes of all sports.
Autologous chondroblasts or chondrocytes2. Cartilage rejuvenation (for high cartilage loss-
preparation treatments are compliant with currentheavy incidents). Mesenchymal stem cells
European Parliament and of the council of 13modification to chondrocytes. Adipose tissue
November 2007 on: Advanced therapy medicinalderived stem cells cultured and differentiated into
products and amending Directive 2001/83/EC andchondrocytes,implanted in three dimensionalscaffold
Regulation (EC) No 726/20042.and infused arthroscopically, for cartilage loss over 3-
2
4 cm .

14. Bone marrow is the part of the body that produces the
hematopoietic stem cells and all the mature blood
elements. Bone marrow transplantation is a medical
procedure whose aim is to replace unhealthy blood stem
cells with healthy in order to treat a blood malignant or
inherited disease.
Bone marrow is selected by iliac crest aspiration and is a
rich source of hematopoietic and mesenchymal stem
cells.
This product is also used to treat solid malignant tumors
or in regenerative medicine.
6.
BONE MARROW
STEM CELLS

15. 7.
DENTAL PULP MESENCHYMAL
STEM CELLS
Mesenchymal stem cells from the dental pulp have the The interaction of mesenchymal stem cells with PRP is a
potential to differentiate into a variety of cell types. promising tool for their therapeutic use in:
Several publications show their importance in tissue
regeneration due to the unique characteristics they
demonstrated such as proliferation, differentiation and
plasticity.
Advantages
ŸEasy & noninvasive procedure
ŸSafe cryopreservation
ŸExtensive proliferation
ŸLong lifespan
ŸHighly efficient
ŸMaximum regenerative ability
Biohellenika developed an innovative technology to bank
stem cells from teeth for people to secure their future
health.
Ÿ
ŸRegenerative medicine of
many organs like:
ŸNerve & spinal cord
ŸBrain
ŸHeart
ŸLiver
ŸBone
ŸLigaments
and cartilage
ŸMuscle
ŸSkin
Regenerative dentistry
Chlidren
Deciduous
teeth
Adults
Wisdom
teeth
Mesenchymal
Stem Cells

16. STEM CELLS FROM UMBILICAL
CORD-PLACENTA UNIT
1. Hematopoietic Umbilical Cord BloodStem Cells. 3. Wharton's Jelly Mesenchymal Cells. Biohellenika
After the ligation of the umbilical cord the placenta developed a patent for a unique and innovative
blood is collected in a blood bag via umbilical cord vein method to collect mesenchymal stem cells from the
puncture. Cord blood contains hematopoietic stem umbilical cord. The main advantages of this method
cells with clinical applications for malignant diseases are the highest number of collected cells, the cost
for more than 20 years. Over 25.000 cord blood effectiveness and a ready to use product. The cells are
hematopoietic stem cells transplantations have been collected from the total length of the cord and
referred until today. cryopreserved in more than of one vials, so there is no
need to proliferate and the cells maintain the
2. Hematopoietic Stem Cells from Placenta stemness and all biological activities during the
Drainage. Many stem cells remain in the placenta storage.
after puncturing the umbilical vein, because of the
The cell therapies are safe because the cells arestructure of the placenta and the conditions of birth. In
autologous (patient's own cells), the procedures arethe emergencies during the delivery, the volume of the
minimally invasive with short recovery time for thecollected placenta blood is low and the drainage of the
patient but with long-lasting results.placenta offers double amount of cells. The stem cells
that are collected by this procedure are enriched in
hematopoietic stem sells, endothelia and pro
endothelia cells and are proposed to improve
angiogenesis after coronary artery occlusion.
8.

18. www.biohellenika.gr
THESSALONIKI PATRA ROMANIA
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CHANIA - CRETE SKOPJERESEARCH FOUNDATION
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OPEN LINE ___ 801.11. 99299
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GREECE INTERNATIONAL OFFICES
SCIENTIFIC CONSULTANTS
K. Kouzi, MD PhD Professor Histology - Embryology, AUTh, Mobile: +30 6944677746
G. Koliakos, MD PhD Professor of Biochemistry, AUTh