1. The chemical factors requireda. Vitaminsb. carbon sourcec. .pdf

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1. The chemical factors required a. Vitamins b. carbon source c. Amino acids d. Balanced salt solution e. Phenol red eye f. HEPES buffer 2. Physical and environmental factors a. Temperature 37oC b. CO2 5% c. relative humidity 95% 4. Focal adhesion: The adhesive contact between the cell and extracellular matrix using the different membrane anchor proteins from the cell. Filopodia: Long slender extensions which extend beyond the leading edge of the lamellipodia. Lamellipodia: It is a flattened extension of the cell with which the cell moves or binds to a surface. Solution 1. The chemical factors required a. Vitamins b. carbon source c. Amino acids d. Balanced salt solution e. Phenol red eye f. HEPES buffer 2. Physical and environmental factors a. Temperature 37oC b. CO2 5% c. relative humidity 95% 4. Focal adhesion: The adhesive contact between the cell and extracellular matrix using the different membrane anchor proteins from the cell. Filopodia: Long slender extensions which extend beyond the leading edge of the lamellipodia. Lamellipodia: It is a flattened extension of the cell with which the cell moves or binds to a surface..

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Lewis Base is a substance that can gain electron . Out of the following H3O+ would have the tendency to lose the electron and not gain another electron . It functions as an acid and not a base. Option - C Solution Lewis Base is a substance that can gain electron . Out of the following H3O+ would have the tendency to lose the electron and not gain another electron . It functions as an acid and not a base. Option - C.

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let 3x mole of Fe react then moles of H2O is 4x moles Fe3O4 formed=x moles of H2=4x Kc=5.1=x*(4x)^4/((0.1-3x)^3(0.05-4x)^4) solving we get x=0.00572 moles of Fe3O4=0.00572 so mass=moles*mol.wt =0.00572(55.845*3+16*4) =1.32 g Solution let 3x mole of Fe react then moles of H2O is 4x moles Fe3O4 formed=x moles of H2=4x Kc=5.1=x*(4x)^4/((0.1-3x)^3(0.05-4x)^4) solving we get x=0.00572 moles of Fe3O4=0.00572 so mass=moles*mol.wt =0.00572(55.845*3+16*4) =1.32 g.

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Usually NGS (Next generation sequencing) is extensively used in understanding the viral genome by generating many billions of sequences. But of late NGS is also finding its place in other areas of science like Transcriptomics where the measurement of mRNA is done to understand the genomic expression better and it’s possible modifications in health and disease.Till now microarray had been used to quantify the gene expression but this is being replaced by NGS of transcribed RNA termed as RNA seq. The key steps include the isolation of RNA and removal of host genomic DNA. Following which, rRNA is also removed by selection of polyadenylated RNA or with antisense oligos. Complementary DNA synthesis is primed either by oligo(dT) or randomly on long transcripts. Analysis of RNA-seq data is usually achieved by mapping sequence reads to a reference genome, in this case the comparison between the infected genome and the normal genome can be done, using software that can map reads over gene splice junctions. Statistical analysis of the number of reads per genome region (gene) can be used to quantify relative levels of expression. Also, Existing ORF and the newly generated ORF can be identified. EV71 infection induces the expressions of genes which are associated with the secretions of inflammatory cytokines, chemokines, complementary proteins and host cell apoptosis. The major signaling pathway affected is the MAPK signaling pathway. Solution Usually NGS (Next generation sequencing) is extensively used in understanding the viral genome by generating many billions of sequences. But of late NGS is also finding its place in other areas of science like Transcriptomics where the measurement of mRNA is done to understand the genomic expression better and it’s possible modifications in health and disease.Till now microarray had been used to quantify the gene expression but this is being replaced by NGS of transcribed RNA termed as RNA seq. The key steps include the isolation of RNA and removal of host genomic DNA. Following which, rRNA is also removed by selection of polyadenylated RNA or with antisense oligos. Complementary DNA synthesis is primed either by oligo(dT) or randomly on long transcripts. Analysis of RNA-seq data is usually achieved by mapping sequence reads to a reference genome, in this case the comparison between the infected genome and the normal genome can be done, using software that can map reads over gene splice junctions. Statistical analysis of the number of reads per genome region (gene) can be used to quantify relative levels of expression. Also, Existing ORF and the newly generated ORF can be identified. EV71 infection induces the expressions of genes which are associated with the secretions of inflammatory cytokines, chemokines, complementary proteins and host cell apoptosis. The major signaling pathway affected is the MAPK signaling pathway..

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Process of transmission between A node and B node: There are different types of frames that rae present to transmission of data. They are Header and response are the two functions are there in the process of transmission. Solution Process of transmission between A node and B node: There are different types of frames that rae present to transmission of data. They are Header and response are the two functions are there in the process of transmission..

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Fluorescence return from excited singlet state to ground state; does not require change in spin orientation (more common of relaxation) Phosphoresence return from a triplet excited state to a ground state; electron requires change in spin orientation Therefore, the rates of fluorescence are several orders of magnitude faster than that of phosphorescence. Solution Fluorescence return from excited singlet state to ground state; does not require change in spin orientation (more common of relaxation) Phosphoresence return from a triplet excited state to a ground state; electron requires change in spin orientation Therefore, the rates of fluorescence are several orders of magnitude faster than that of phosphorescence..

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Ion channels are the membrane proteins which have pores. They allow passage of ions through its pores and are responsible for creating membrane potential, and voltage-gated electrical signals. Ion channels, hence control the cellular volume through the secretions from various cells. Some of the ion channels are: sodium-potassium pump, sodium-calcium exchanger, sodium-glucose transport proteins etc. Genomic evolution has brought about transformation in the function and structure of the ion channels. These channels have existed even before the existence of animals. The presence of ion channels in cnidarians like sea anemones, corals, jellyfish and others have shed light on the evolution of ion channels. Ion channels have formed by the modification of the prokaryotic porins, colicins, ABC transporters. They can be catagorised into families and sub-families like the voltage-gated channels and messanger-gated channels. Of these two, ligand-gated channels have evolved recently. Moreover, Na+ and Ca2+ channels genes have evolved from K+ channel genes, brought about by duplication and mutation. Solution Ion channels are the membrane proteins which have pores. They allow passage of ions through its pores and are responsible for creating membrane potential, and voltage-gated electrical signals. Ion channels, hence control the cellular volume through the secretions from various cells. Some of the ion channels are: sodium-potassium pump, sodium-calcium exchanger, sodium-glucose transport proteins etc. Genomic evolution has brought about transformation in the function and structure of the ion channels. These channels have existed even before the existence of animals. The presence of ion channels in cnidarians like sea anemones, corals, jellyfish and others have shed light on the evolution of ion channels. Ion channels have formed by the modification of the prokaryotic porins, colicins, ABC transporters. They can be catagorised into families and sub-families like the voltage-gated channels and messanger-gated channels. Of these two, ligand-gated channels have evolved recently. Moreover, Na+ and Ca2+ channels genes have evolved from K+ channel genes, brought about by duplication and mutation..

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