virology , influenza virus

1. Influenza Virus
Infection
Ahmad Kabrah
Ayoub Al Othaim
Wassem Al-zamzami

2. Outline
• Etiology
• Virus structure
• Type of Influenza
• Antigenic shift and
drift
• Pathogenesis
• Clinical features
• Symptoms
• Complication
• Transmission
• Epidemiology
• Diagnosis
• Treatment
• prevention
• Influenza vaccine

3. Etiology
• There are three genera of influenza viruses: influenza
virus A, influenza virus B and influenza virus C.
• These viruses are also called type A, type B and type C
influenza viruses.
• This subtype based on hemagglutinin (H) and
neuraminindase (N) protein.

4. Virus structure
• This virus consists of segmented pieces of negative-sense RNA,
each piece containing either one or two genes which code for a
gene product (protein).
• It has 8 pieces of genes.
• It is an enveloped virus.
• It contains 2 surface
glycoprotein.

5. Influenza A viruses
• Influenza A viruses include avian, swine, equine and canine
influenza viruses, as well as the human influenza A viruses
• Influenza A viruses are classified into subtypes based on two
surface antigens, the hemagglutinin (H) and neuraminidase
(N) proteins.
• There are 16 hemagglutinin antigens (H1 to H16) and nine
neuraminidase antigens (N1 to N9).
• Only H 1, 2 and 3, and N 1 and 2 are commonly found in
humans.
• Current subtypes of influenza A viruses found in people are
influenza A (H1N1) and influenza A (H3N2) viruses.

6. Influenza A viruses
• Swine influenza is a respiratory disease of pigs causes by type
A influenza virus.
• Swine influenza viruses changing among swine and causes
new virus can infect humans.
• Influenza change may be occur by antigenic drift or antigenic
shift.

7. Antigenic shift and drift in
influenza A viruses
Antigenic Drift
• It is small changes in the virus that happen continually and
slowly over time. Antigenic drift produces new virus strains
that may not be recognized by the body's immune system. This
is one of the main reasons why people can get the flu more
than one time.
• In most years, one or two of the three virus strains in the
influenza vaccine are updated to keep up with the changes in
the circulating flu viruses. So, people who want to be protected
from flu need to get a flu shot every year.

8. Antigenic Shift
• Antigenic shift results when two different flu strains combine
and infect the same cell.
• It is an abrupt, major change in the influenza viruses, resulting
in new hemagglutinin and/or new hemagglutinin and
neuraminidase proteins in influenza viruses that infect
humans.
• Shift allows flu viruses to move from animals to humans. An
example of that is H1N1 virus which occurred in the spring of
2009.

10. Influenza B viruses
• Influenza B viruses are known to circulate only in human
populations.
• These viruses can cause epidemics, but they have not, to date,
been responsible for pandemics.
• They have also been found rarely in animals.
• Influenza B viruses are categorized into lineages rather than
subtypes.
• They are also classified into strains.
• Influenza B viruses undergo antigenic drift, though it occurs
more slowly than in influenza A viruses.

11. Influenza B viruses
• Until recently, the B/Victoria/2/87 lineage predominated in
human populations, and influenza B viruses were said not to
undergo antigenic shifts.
• However, recent evidence suggests that recombination
between B/Yamagata/16/88 lineage, which developed in
various parts of the world in 2001, and B/Victoria/2/87 lineage
is resulting in antigenic shifts.
B/Yamagata/16/88 lineage + B/Victoria/2/87 lineage
recombination
antigenic shifts

12. Influenza C viruses
• Influenza C viruses circulate in human populations, and are
mainly associated with disease in people.
• Until recently, they had never been linked to large-scale
epidemics.
• Influenza C viruses have also been found in animals.
• They are classified into strains. Each strain is antigenically
stable, and accumulates few changes over time.

13. Pathogenesis
• Following respiratory transmission, the virus
attaches to and penetrates respiratory
epithelial cells in the trachea and bronchi.
• Viral replication occurs, which results in the
destruction of the host cell.
• Viremia has rarely been documented.
• Virus is shed in respiratory secretions for 5–
10 days.

15. Clinical Features
• Incubation period 2 days (range 1-4 days)
• Severity of illness depends on prior
experience with related variants
• Abrupt onset of fever, myalgia, sore throat,
nonproductive cough, headache

16. Symptoms
• Fever
• Cough
• Runny nose
• Body aches
• Sore throat
• Chills
• Headache
• Fatigue

17. Influenza Complications
 Many people with flu have had vomiting and diarrhea.
 Flu can cause neurologic symptoms in children can be
very severe often fatal.
 The severity of symptoms sometimes require
hospitalization.
 In some cases, severe complications such as pneumonia
and respiratory failure can cause death.
 Death 0.5-1 per 1,000 cases

18. Transmission

19. Influenza Epidemiology
• Reservoir - Human, animals (type A only)
• Transmission - Respiratory Probably
airborne
• Temporal pattern - Peak December - March
in temperate climate May occur earlier or
later
• Communicability - 1 day before to 5 days
after onset (adults)

20. Impact of Influenza - United
States

21. Impact of Influenza
• Highest rates of complications and
hospitalization among young children and
person > or =65 years
• Average of >200,000 influenza-related excess
hospitalizations
• 57% of hospitalizations among persons <65
years of age
• Greater number of hospitalizations during
type A (H3N2) epidemics

22. Diagnosis
• The diagnosis of influenza is usually suspected on the basis of
characteristic clinical findings, particularly if influenza has
been reported in the community.
• Human influenza A and influenza B infections can be
diagnosed by virus isolation or by the detection of antigens or
nucleic acids .
• The viruses can be isolated in cell lines or chicken embryos
with identification by hemagglutination and neuraminidase
inhibition tests or RT-PCR
• Antigens can be detected in respiratory secretions by
immunofluorescence or enzyme-linked immunosorbent assays
(ELISAs).

23. Diagnosis
• Serologic confirmation of influenza requires demonstration of
a significant rise in influenza IgG.
• Complement fixation (CF) and hemagglutination inhibition (HI)
are the serologic tests most commonly used

24. TREATMENT
• Most people with the flu recover within one to two weeks
without treatment. However, serious complications of the flu
can occur.
• Supportive care for uncomplicated influenza in humans
includes fluids and rest .
• More severe cases, or infections that have an elevated risk of
complications, may be treated with antiviral drugs .
• Four drugs - amantadine, rimantadine, zanamivir and
oseltamivir - are used to treat influenza.

26. Outbreak detection
• The viruses were first identified in U.S. pigs in 2010.
• In 2011, 12 cases of H3N2v infection were detected in the
United States (Indiana, Iowa, Maine, Pennsylvania, and West
Virginia).
http://modernsurvivalblog.com/wp-content/uploads/2012/01/swine-flu-h3n2v.jpg

27. Recent Outbreak Detection
In 2012:
• 309 infections with an influenza A (H3N2) variant
(H3N2v) were identified in 12 states.
• During the 2012 outbreak, most cases reported
agricultural fair attendance and contact with swine prior
to illness.

28. States Reporting
H3N2v Cases
Cases in
2011
Cases in
2012
Cases in
2013
Hawaii 1
Illinois 4 1
Indiana 2 138 14
Iowa 3 1 1
Maine 2
Maryland 12
Michigan 6 2
Minnesota 5
Ohio 107 1
Pennsylvania 3 11
Utah 1*
West Virginia 2 3
Wisconsin 20
Total 12 309 19
Detected U.S. Human Infections with H3N2v by State from August 2011 to October 18, 2013
* Case in Utah occurred in April 2012. CDC,2013

29. Outbreak Detection
• Confirmed H3N2v cases were identified primarily among
children (<18 years of age), and limited serologic studies
indicate that children, primarily those younger than 9
years, have increased susceptibility to H3N2v. However,
some adult H3N2v cases were identified.

30. Influenza virus vaccine
• A vaccine is a biological preparation that improves immunity
to a particular disease.
• Two types of influenza vaccine are available in the United
States. Trivalent inactivated influenza vaccine (TIV) and Live
attenuated influenza vaccine (LAIV).

32. History of Vaccine

33. Vaccine effectiveness
• Effectiveness is variable from year to year and among
populations
•  Generally lower than routine EPI vaccines
 Factors that affect true vaccine effectiveness
•  Antigenic relatedness between vaccine virus to circulating
strains
•  Host factors
 Age (immune responses in very young and very old) 
Underlying illnesses
•  Vaccine type
 Programmatic issues

34. Challenges
• Expanding or introducing influenza vaccine programs is
challenging:
• Variety of vaccine products/types
• Developing policy requires a solid evidence base
• (e.g. risk groups, timing of campaign)
• Most risk groups are not those targeted by routine EPI
vaccines – new partners, training, etc.
• Communicating value is complicated
• Need for annual vaccination

35. Gaps
• Most partners need education on the need / value of the
vaccine
• Relative benefit of annual campaigns in countries with
substantial year-round disease is poorly understood
• Relatively few data on the performance of vaccine in
developing country populations
• Disease burden and economic burden (or CE of vaccine)
poorly understood in many places (but data being generated
quickly)
• NRAs gaining experience with approval of influenza vaccines

36. Opportunities
• New developers and producers of influenza vaccines
• – Capacity has increased dramatically and will continue to
• Interest in influenza prevention post-pandemic – Will wane
quickly though
• Substantial and high quality surveillance and disease burden
data now available for most countries
• New WHO SAGE recommendations to use vaccine

37. References
• Influenza Vaccines for the Future
Linda C. Lambert, Ph.D., and Anthony S. Fauci, M.D.
• http://www.who.int/influenza/GIP_InfluenzaVirusInfectionsHu
mans_Jul13.pdf?ua=1.
• CDC.com
• Medical Microbiology and Immunology 8th edition
• http://www.cdc.gov/flu/about/viruses/types.htm