If enzymes resemble a scientific or economic bottleneck, a unique expression system can help: Our proprietary cell surface display technologies MATE and Autodisplay combine the benefits of soluble enzymes and whole cell biocatalysts. Enzymes are naturally immobilized on the surface of gram negative bacteria. The reaction is facilitated on the outside of the cell – no transport across the membrane is needed. This means there are virtually no limitations regarding substrate or product (toxicity or size). We have broad expertise with especially challenging enzymes such as multimeric enzymes (e.g. nitrilases) or enzymes that require cofactors (e.g. P450 enzymes). Find out how Autodisplay Biotech's custom-tailored products and services can contribute to driving your R&D activities to the next level.

1. ask
yourself
THE FOLLOWING
QUESTIONS!

2. HAVE ENZYMES EVER
BEEN A LIMITING FACTOR IN
YOUR PROCESSES?
1

3. HAS INTRACELLULAR
EXPRESSION OF ENZYMES
BEEN A BOTTLENECK?
2

4. HAVE YOU EVER
WISHED, TO BE ABLE TO
RECOVER ENZYMES FROM
YOUR PROCESSES?
3

5. WAS IMMOBILISATION OF
ENZYMES DISCARDED BECAUSE
OF HIGH COSTS?
4

6. THERE IS
ONE
OPTION

7. COMBINING THE POSITIVE
CHARACTERISTICS OF
free
enzymes
whole cell
biocatalysts
immobilisation
WHILE RETAINING
MAXIMUM FLEXIBILITY

8. NATURAL IMMOBILISATION OF
ENZYMES THROUGH…
bacterial
surface
display

9. OUR TECHNOLOGY…
OM
PP
IM
CP
SP
PASSENGER
LINKER
β-BARREL

10. … IS A SELF-SUFFICIENT SYSTEM
OM
PP
IM
CP
SP
PASSENGER
LINKER
β-BARREL

11. WHAT ARE THE BENEFITS

12. FOR A COST-EFFICIENT
PURIFICATION OF ENZYMES…
1
simply
centrifuge or
filter

13. FOR THE RECOVERY OF ENZYMES
FROM A CONVERSION PROCESS…
2
simply
centrifuge or
filter

14. NITRILASE EXPERIMENTS PROVE:
RECOVERY IS SUCCESSFUL
CONVERSION
100%
70%
50%
30%
SCHEMATIC DRAWING
10%
#1
#2
#3
#4
#5
CYCLES
@24h each

15. UNLOCK ENHANCED ENZYME
STABILITY THROUGH…
3
natural
immobilisation

16. ENABLE METABOLIZATION OF
ENZYME INHIBITORS THROUGH…
4
smart
choice of host
organisms

17. RELEASE ENZYMES EXPRESSED
WITH AUTODISPLAY THROUGH…
5
integrated
cleavage sites
(& HIS-tag)

18. CONCENTRATION OF RELEASED
ENZYMES IS SIMPLE
1
2
N
FILTRATE
resuspendent
cells
HIGH CELL DENSITY
FERMENTATION
CONCENTRATED
SUPERNATANT

19. you
may
ask
HOW MANY
MOLECULES ARE LOCATED
ON THE SURFACE?

20. 100,000
–
200,000
MOLECULES/CELL

21. HELP
WE CAN
, WHEN
OTHER SYSTEMS FAIL
ESPECIALLY IN THE CASE OF
CHALLENGING ENZYMES

22. you
may
ask
WHAT ARE
CHALLENGING
ENZYMES?

23. ALL ENZYMES WHICH ARE
DIFFICULT TO HANDLE, DUE TO…
inclusion
bodies
incorrect
folding
multimerisation
lack of
stability
co-factors

24. WE ARE DEDICATED TO SOLVE
BIOCATALYTIC BOTTLECKS
1
ENZYME PROBLEM
GACTTCGAAGTC
TTTAAGAATGACT
TCGAAGTCTTTAT
2
NAME THE ENZYME
GENE SEQUENCE
3
gram –
4
WHOLE CELL
BIOCATALYST
HOST
ORGANISM
+
PLASMID

25. RECAP: VALUE PROPOSITION
enzyme
stability
enzyme
recovery/
re-use
enzyme
costs
enzyme
purification
enzyme
portfolio
sales and
profitability

26. TALK TO US, IF YOU
WANT TO KNOW MORE