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INTRODUCTION TO
APPLIED &
ENVIRONMENTAL
MICROBIOLOGY
Chapter 1
BIOM 430 Spring 2013
Gill Geesey
Assignment #1
 Text reading assignment: Chapter 1 & 2
Applied Microbiology
(Biotechnology)
 The exploitation of microorganisms, their
activities, and their products for the
benefit of society.
 Beverage production
 Dairy products
 Bioremediation of disturbed environments
 Antibiotics
Environmental Microbiology
 The study of microorganisms and their
activities in the environment for the
purpose of understanding their role in
maintenance of the biosphere
 Cycling of elements and nutrients
 Influence on global climate
Microbial Ecology
 Microbial Ecology is the study of
interactions between populations of
microorganisms and between microbial
populations and organisms in other tropic
levels
Key events that led to the development of
Environmental Microbiology as a scientific
discipline
 Rachael Carson published “Silent Spring”
bringing to the public’s attention the poor
condition of the environment
 Emergence of new waterborne and
foodborne pathogens that posed human
health threats
 New technologies have emerged to study
microbes in their natural environment rather
than as pure cultures in the laboratory
Applied &
Environmental
Microbiology
Soil
microbiology
Aero-
microbiology
Hazardous
waste/
bioremediation
Aquatic
microbiology
Water quality
Industrial
microbiology
Food safety
Occupational
health/
Infection
control
Diagnostic
microbiology
Biotechnology
MICROORGANISMS IN THE
ENVIRONMENT
 They are ubiquitous – occur everywhere
 In and on the body
 10 times more microbial cells than our own cells in
the normal healthy human body
 Things we touch, use, eat and drink
 Wet surfaces – biofilms
 Plant tissue surfaces
 Deep subsurface environment
 Gold mines, oil reservoirs
Microorganisms in the Environment
 Viruses – animal & plant pathogens,
bacteriophages
 Prior to the advent of antibiotics,
bacteriophage were being studied as
antibacterial agents. Is this concept still
applicable today?
 Bacteria – pathogens & commensals;
many are saprophytic, some
chemolithotrophic, some photosynthetic
Microorganisms in the Environment
 Archaea
 Algae – primary producers, some
pathogens
 Fungi – from microscopic to macroscopic
 Protozoa – parasites & saprophytes
Environmental Monitoring
 Challenge:
 Microbes exist as complex communities-not as
pure cultures in the natural environment
 Defining microbial community structure and
function under in-situ conditions is just now
becoming possible
 Isolating microorganisms from their natural
state and studying them in an artificial lab
environment is no longer necessary. Why?
 Isolation of one population from another
consorting population may be impossible...
Monitoring approaches
 Molecular methods... Are they the gold
standard?
 No one approach is free of bias
 Best strategy is to use several independent
approaches and compare results
Modern Environmental Microbiology
 Discovery and identification of new microbes,
microbial activities, and microbial products
that protect environment
 Use microbial products as pesticides to replace
chemical pesticides with long resident time in
environment. Example of such a product?
 Microbially enhanced oil recovery. Example of a
product?
 Microbially enhanced mineral recovery. Example?
 Bioremediation
Modern Environmental Microbiology
 New steps in the cycling of elements
 Anaerobic oxidation of ammonia
 Anaerobic oxidation of methane
 Archaea are much more abundant in the
worlds oceans than initially thought
 What roles do they play there?
Goals of the course
 Gain an appreciation for the good
things microbes do in the biosphere
 Appreciate the need to maintain
microbial diversity in the biosphere
 Learn connection between human
health and the health of the
environment-both near and far.

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Chapter 1.ppt

  • 2. Assignment #1  Text reading assignment: Chapter 1 & 2
  • 3. Applied Microbiology (Biotechnology)  The exploitation of microorganisms, their activities, and their products for the benefit of society.  Beverage production  Dairy products  Bioremediation of disturbed environments  Antibiotics
  • 4. Environmental Microbiology  The study of microorganisms and their activities in the environment for the purpose of understanding their role in maintenance of the biosphere  Cycling of elements and nutrients  Influence on global climate
  • 5. Microbial Ecology  Microbial Ecology is the study of interactions between populations of microorganisms and between microbial populations and organisms in other tropic levels
  • 6. Key events that led to the development of Environmental Microbiology as a scientific discipline  Rachael Carson published “Silent Spring” bringing to the public’s attention the poor condition of the environment  Emergence of new waterborne and foodborne pathogens that posed human health threats  New technologies have emerged to study microbes in their natural environment rather than as pure cultures in the laboratory
  • 8. MICROORGANISMS IN THE ENVIRONMENT  They are ubiquitous – occur everywhere  In and on the body  10 times more microbial cells than our own cells in the normal healthy human body  Things we touch, use, eat and drink  Wet surfaces – biofilms  Plant tissue surfaces  Deep subsurface environment  Gold mines, oil reservoirs
  • 9. Microorganisms in the Environment  Viruses – animal & plant pathogens, bacteriophages  Prior to the advent of antibiotics, bacteriophage were being studied as antibacterial agents. Is this concept still applicable today?  Bacteria – pathogens & commensals; many are saprophytic, some chemolithotrophic, some photosynthetic
  • 10. Microorganisms in the Environment  Archaea  Algae – primary producers, some pathogens  Fungi – from microscopic to macroscopic  Protozoa – parasites & saprophytes
  • 11. Environmental Monitoring  Challenge:  Microbes exist as complex communities-not as pure cultures in the natural environment  Defining microbial community structure and function under in-situ conditions is just now becoming possible  Isolating microorganisms from their natural state and studying them in an artificial lab environment is no longer necessary. Why?  Isolation of one population from another consorting population may be impossible...
  • 12. Monitoring approaches  Molecular methods... Are they the gold standard?  No one approach is free of bias  Best strategy is to use several independent approaches and compare results
  • 13. Modern Environmental Microbiology  Discovery and identification of new microbes, microbial activities, and microbial products that protect environment  Use microbial products as pesticides to replace chemical pesticides with long resident time in environment. Example of such a product?  Microbially enhanced oil recovery. Example of a product?  Microbially enhanced mineral recovery. Example?  Bioremediation
  • 14. Modern Environmental Microbiology  New steps in the cycling of elements  Anaerobic oxidation of ammonia  Anaerobic oxidation of methane  Archaea are much more abundant in the worlds oceans than initially thought  What roles do they play there?
  • 15. Goals of the course  Gain an appreciation for the good things microbes do in the biosphere  Appreciate the need to maintain microbial diversity in the biosphere  Learn connection between human health and the health of the environment-both near and far.