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MyREF_Plant Biotechnology_A better future for malaysia_umaiyal munusamy
- 1. Plant Biotechnology: A better future for Malaysia By Ts. Dr. Umaiyal Munusamy Founder of Sayor Mine Network
- 2. Audience Engagement I would like to invite you to the Doodle poll “PLANT BIOTECHNOLOGY: A BETTER FUTURE FOR MALAYSIA”. Please follow the link - https://doodle.com/poll/a6hxx8ncz4zcdrf8
- 6. What is Plant Biotechnology Plant biotechnology is a set of TECHNIQUES used to MODIFY for specific needs or opportunities
- 8. WHY PLANT BIOTECHNOLOGY IS IMPORTANT Assist in developing crops with multiple durable resistance to pests and diseases Reduction in pesticides and herbicides The genetic base of crop production can be preserved and widen by an integration of biotechnology tools in conventional breeding. Can provide us with various food with different quality The raw material for various industries
- 9. WHY CAN BE A FUTURE IN PLANT BIOTECHNOLOGY
- 11. Agriculture remains an important sector of Malaysia's economy, contributing 7.4 percent to the national GDP and providing employment for 10.28 percent LATEST DATA
- 12. THE PLANT RUBBER COCOA OIL PALM BANANA
- 13. COCONUT DURIAN PINEAPLES RAMBUTAN RICE Tropical logs Tropical timber Vegetables
- 14. FUN ACTIVITIES I would like to invite you to the Doodle poll “What tropical fruits do you like to eat?”. Please follow the link - https://doodle.com/poll/ftcwpc8cwr43waf2
- 16. ✓ Genomics-assisted breeding. ✓ Uses a powerful multiplexed approach ✓ Enables users to interrogate hundreds to thousands of markers. Enables hundreds to thousands of samples simultaneously. ✓ It includes NGS-based assays designed using customer- provided markers or catalog panels designed with publicly available markers. Targeted genotyping by sequencing (GBS)
- 17. A unique technology that enables to edit parts of the genome by removing, adding or altering sections of the DNA sequence. CRISPR-Cas9 https://drive. google.com/fi le/d/17M6vto RkbOA1BZWQ nl9tBEK6LphN 45N9/view?us p=sharing
- 18. The biological process of mRNA degradation induced by complementary sequences double- stranded (ds) small interfering RNAs (siRNA) and suppression of target gene expression. RNA interference (RNAi)
- 20. ZINC FINGER NUCLEASE TECHNOLOGY DNA-Binding Activity It is important for DNA-binding activity in plants. Plant C2H2-type zinc finger proteins have unique structural features: the conserved, unique QALGGH (Q-type C2H2) motif and the long variable spacers between adjacent zinc finger domains. A mutation of any amino acid in QALGGH sequence except Q will cause the protein to completely lose its ability to bind to DNA, whereas a mutation in Q will greatly reduce its DNA binding ability. RNA-Binding Activity C2H2-type zinc finger proteins can recognize RNA. Zinc fingers comprehensively recognize specific bases and specific folding backbones in diverse RNA structures. Contact with the phosphoric acid skeleton in RNA is essential for the recognition of this molecule. A phage display assay showed that the amino acids at positions −1 and +2 of the α-helix in C2H2 zinc finger proteins are important for RNA binding. Protein-Binding Activity Zinc finger proteins interact with other zinc finger proteins or other types of protein to regulate target gene expression. Zinc finger proteins interact with other zinc finger proteins to bind to other DNA sequences or to prevent binding to the corresponding DNA sequences, thereby regulating gene transcription and expression, the potential for mediating protein interactions is much larger compared to DNA binding.
- 22. OLIGONUCLEOTIDE DIRECTED MUTAGENESIS ✓ A non-transgenic base pair-specific precision gene editing platform that has been significantly advanced through Rapid Trait Development System (RTDS) to achieve novel and commercially valuable traits in agriculturally important crops. ✓ The RTDS technology harnesses the cell's normal DNA repair system to edit specific targeted bases within the genome through the use of chemically synthesized oligonucleotides. ✓ These oligonucleotides are used as repair templates to generate mismatches in the DNA at the target site. ✓ Through homology-directed pairing between the oligonucleotide and the DNA of the target region, the cell's repair machinery is directed to those sites to correct the mismatched base(s) guided by the oligonucleotide sequence. ✓ Once the correction process is completed, the oligonucleotide is degraded by the cell through natural processes.
- 24. CISGENESIS, TRANSGENESIS AND INTRAGENESIS Cisgenesis ✓ Transfer of genes between crossable species or the plant itself ✓ New trait without foreign gene ✓ No environmental risk ✓ free of markers and any vector backbone ✓ ncludes the gene of interest flanked by its own promoter and terminator, Transgenesis ✓ Transfer of genes between non- crossable species or the plant itself ✓ Recombinant DNA ✓ New trait with foreign gene ✓ No environmental risk Intragenesis ✓ free of markers and any vector backbone ✓ gene of interest can be combined with regulatory elements from the species itself or from a cross compatible species
- 25. Recent cisgenesis and intragenesis-based products include Zea mays and B. napus
- 27. Started 1902
- 28. ORGANOGENESIS single tissue organ is developed into a completely new plant Direct method (explants to shoots) Indirect method explants to callus to shoots) SOMATIC EMBRYOGENESIS Tissues to embryo to plant
- 29. PROSPECTIVE OF PLANT TISSUE CULTURE
- 31. ISSUES ✓ Halal ✓ Animal gene
- 32. Is Plant Biotechnology: A better future for Malaysia ?????? YES YES YES YES