A brief overview of WPI's Fiber Optic Products

1. OEM Fiber Optic Products
Mathias Belz, Tiana L. Riggi
World Precision Instruments Germany GmbH, Pfingstweide. 16, D-61169 Friedberg,
Germany
*TransMIT GmbH, Pfingstweide 16, D-61169 Friedberg, Germany
Mathias Belz et al.

2. Overview
2Mathias Belz et al. Sample Cells (Cuvette & LWCC)
 Introduction to WPI
 Qualified Fibers
 DIP-DUV Transflectance Probe
 Fiber Optic Flowcells
 Applications

3. World Precision Instruments
3Mathias Belz et al. Sample Cells (Cuvette & LWCC)
 Manufacturer of laboratory equipment and optical
sensing tools for the life sciences since 1967
 Offices in USA, Germany, United Kingdom, China and
Brazil
 Start of Center of Excellence for optical sensing in
cooperation with the University of Applied Sciences
Mittelhessen in Friedberg, Germany since 2011
 Move to R&D and production facility June 2019
 New Product Development focus in Germany: optical
flowcells, LED-based detection systems, fluorometer,
fiber optic sensors

4. 4Mathias Belz et al. Sample Cells (Cuvette & LWCC)
 Hersteller von Laborgeräten und optischer
Messtechnik für die Life Sciences seit 1967
 Niederlassungen in USA, Deutschland, UK, China &
Brazilien
 Entwicklungszentrum für Optische Sensorik in
Kooperation mit der Technischen Hochschule
Mittelhessen seit 2011
 2017 Umzug von Berlin nach Friedberg: Verkauf,
Entwicklung und Produktion
 Entwicklungfokus in Deutschland: Optische
Flusszellen, LED-Detektorsysteme, Fluorometer,
optische Sensoren und Life Science Applikationen

5. OEM Fiber & Components
• Qualified and Specialty Fiber
• Fluorescence Probes
• Fiber Coupled Flow Cells
• Custom Fiber Assemblies
• Optical Sensors
• Fiber Coupled LED Light
Sources
5
Mathias
Belz et al.

6. What is an Optical Fiber?
 Is a flexible medium that transfers light from one place to another
 Consists of a highly transparent core responsible for light guidance, a cladding
and a coating,
 A coating protects the fiber from strain, heat and water, which can make it
brittle
core
coating /
overcoat
cladding
6
Mathias
Belz et al.

7. 500
Wavelength [nm]
Attenuation[dB/km]
400
500 1000 1500 2000
0
200
400
600
800
1000
20001000 1500
0
800
600
200
1000
500 1000 1500 2000
0
200
400
600
800
1000
B
A
FVP
High OH
low OH
Attenuation[dB/m]
0.4
0
0.8
0.6
0.2
1.0
All-silica fiber:
low & high OH-content

8. Smallest Fiber Optic Dipping Probe
Designed for Spot checking Handhelds
 Perfect for UV/VIS Spectroscopy from 230 nm to 1000 nm
 Tip diameter 1.5mm, Light Pathlength 2, 5, 10 mm, Assembly
Length 1m,
 SMA 905 connector, Launch and return fiber bundle (7x200um)
680um*
 Requires 20-50 µL sample in 200 µL centrifuge vial
 Compatible with most fiber optic spectrometers
Miniature transmission probe for microliter sampling
Mathias Belz et al. 8

9. Smallest Fiber Optic Dipping Probe
 Standard solutions of DNA were gravimetrically using 18.2MΩ/cm
ultrapurified water
 Solutions were prepared between 0.0µg/mL and 687.6µg/mL
Miniature transmission probe for microliter sampling
Designed for Spot checking Handhelds
Mathias Belz et al. 9

10. New DIP-DUV Transflectance Probe
UV-optimized for 200 – 250 nm: Lab use and simple process applications
 Perfect for UV/VIS Spectroscopy from
200 nm to 1000 nm
 Fused silica windows
 Light Pathlength 2 & 10 mm,
Assembly Length 1.5 m,
 SMA 905 connector, 600 µm launch
and return fiber
 Compatible with most fiber optic
spectrometers
Mathias Belz et al. 10

11. New DIP-DUV Transflectance Probe
Transmission in Air & MilliQ Water versus 2x600 µm butt-coupled fibers
 Perfect for UV/VIS
Spectroscopy from 200
nm to 1000 nm
 Minimized liquid
obstruction in optical
path
0
5
10
15
20
25
30
35
40
45
200 250 300 350 400 450 500 550 600 650 700
Transmission[%]
Wavelength [nm]
Air MilliQ Water
Mathias Belz et al. 11

12. New DIP-DUV Transflectance Probe
Linearity of DIP-DUV Probe at 520 nm
 No difference to
cuvette measurement
R² = 0.9977
R² = 0.9998
R² = 1
0
0.5
1
1.5
2
2.5
3
0 0.5 1 1.5 2 2.5 3
TR-ProbeAbsorbance[AU]
Cuvette Absorbance [AU]
to 2.6 AU/cm
to 2.0 AU/cm
to 1.5 AU/cm
Linear (to 2.6 AU/cm)
Mathias Belz et al. 12

13. New DIP-DUV Transflectance Probe
Baseline offset with MeOH and 32 ppt NaCl against MilliQ water
 Exceptional low
baseline offset
 Virtually flat -0.015
-0.01
-0.005
0
0.005
0.01
0.015
200 300 400 500 600 700
Absorption[AU]
Wavelength [nm]
MilliQ Water NaCl MeOH
Mathias Belz et al. 13

14. Liquid Waveguide Capillary Cells
Longpass flowcells for absorbance measurements
 Path Length 10 - 500 cm
 Internal Volumes: 125 uL – 3.1 mL
 Inner Diameter: 550 um or 2.0 mm
 Wavelength Range: 230 nm to 1000
nm
 Sold Commercially since 1996
 10-32 coned liquid port fittings
 SMA 905 Connectors
Mathias Belz et al. 14

15. Nutrient Analysis: Phosphate
Reproduced from Gimbert et al. Talanta, 71 (2007): 1624-1628. 15

16. Nutrient Analysis: Phosphate
Reproduced from Zhang, Chi. Environ Sci Technol. 2002 Mar 1;36(5):1048-53. 16

17. Liquid Waveguide Capillary Cells
New Optical Flow cells
 Path Length
1 - 500 cm
 Inner Diameter
400, 550,
1000, 2000 µm
Flow Cell – Type A & B
DetectorLight Source
Optical
Fiber
Fused Silica
Capillary
Low RI coating
Optical
Fiber
 Wavelength Range:
(185) 230 nm to
1000 nm
 Internal
Volumes:
1 µL – 3.1 mL
Mathias Belz et al. 17

18. NEW Liquid Waveguide Capillary Cells
LWCC-5010: Transmission 200 to 720 nm with MilliQ Water
 Pathlength
10 cm
 Inner Diameter
1 mm
 Internal Volume
80 µL
 Transmission
measured versus 2x
600 µm butt-coupled
fibers
Mathias Belz et al. 18

19. NEW Liquid Waveguide Capillary Cells
LWCC-5010 – Exceptional DUV Transmission 185 to 250 nm with MilliQ Water
 Pathlength
10 cm
 Inner Diameter
1 mm
 Internal Volume
80 µL
 Transmission
measured versus 2x
600 µm butt-coupled
fibers
Mathias Belz et al. 19

20. NEW Liquid Waveguide Capillary Cells
LWCC-5010: Intensity Profile
 Pathlength
10 cm
 Inner Diameter
1 mm
 Internal Volume
80 µL
 Intensity profile
measured with TIDAS
1 spectrometer and
D4H Lightsource
Mathias Belz et al. 20

21. NEW Liquid Waveguide Capillary Cells
LWCC-5010 – 34 PPT NaCl Baseline Offset against MilliQ Water
 Pathlength
10 cm
 Inner Diameter
1 mm
 Internal Volume
80 µL
 Transmission
measured versus 2x
600 µm butt-coupled
fibers
Mathias Belz et al. 21

22. NEW Liquid Waveguide Capillary Cells
LWCC-5010 – Methanol Baseline Offset against MilliQ Water
 Pathlength
10 cm
 Inner Diameter
1 mm
 Internal Volume
80 µL
 Transmission
measured versus 2x
600 µm butt-coupled
fibers
Mathias Belz et al. 22

23. NEW Liquid Waveguide Capillary Cells
Linearity of Type A, B & LWCC-M-100 measured with Azurbin Red @ 520 nm
Mathias Belz et al. 23

24. BioFlowCell
Single use flowcell for spectroscopic analysis in bio processes
 Optical detection systems for bioprocesses are commonly made for
stainless steel reactors with probes, light guides and spectrometers.
 State of the art systems are sterilizable in place by steam.
 This is not possible for disposable bioreactors, as they are commonly
pre-sterilized by γ-radiation and a probe cannot be installed afterwards
without breaking a sterile barrier.
 WPI’s disposable BioFlowCells can be Gamma sterilized and are
designed for disposable bioreactor systems.
Mathias Belz et al. 24

25. BioFlowCell
Typical Experimental Setup
inflow
outflow
bio reactor
detection fiber
LED driver
data
current control
settings
& trigger
data
data
settings
& triggerdata
Settings & trigger
disposable
flowcell
fiber(s) for light
scattering
emission fiber
absorption
detector unit
Scattering
detector unit
monitor
photdiode
multi LED
unit
PC or micro
computer with
user interface
Mathias Belz et al. 25

26. BioFlowCell
Bio Process control for disposable bioreactors in Bypass
 2.5, 5, 10, 20mm Single Use
Flowcell
 Additive production technique
 Fused silica windows
 Sterile
 Compatible to stainless steel
reactors and disposable bio
reactors.
 Next Product: 2 pathlength and 4
pathlength flowcell
Mathias Belz et al. 26

27. BioFlowCell Holder
Fiber Optic Holder for single pathlength BioFlowCells
 Adapts single pathlength
BioFlowCell to Optical detector
 Additive production technique,
ABS
 Non- sterile, re-usable
 SMA 905 connection
 200 nm to 1000 nm
 Next Product: Holder for 2
pathlength and 4 pathlength
flowcell
Mathias Belz et al. 27

28. CHO – cell cultivation
Light scattering at 0, 20 and 90 degree BioFlowCells
 Observe cell grows continuously
by scattered light at 850 nm
Mathias Belz et al. 28

29. BioFlowCell Design
Schematic of Multipathlength & Scattering Flowcell
 Absorbance at 260,
280, 340, 450 nm &
 Light scattering at 0,
20 and 90 degree
Mathias Belz et al. 29

30. Conclusions
New Fibers, Dipping Probes and Flowcells with excellent UV Transmission
 World Precision Instruments (WPI) manufactures and resells
laboratory equipment for the life sciences
 WPI GmbHs R&D focus is optical sensing technology
 New Transflectance Probe with > 10% UV Transmission @ 200
nm & excellent linearity
 New Longpath Flowcell with UV Transmission > 5% @ 190 nm
and >10% @ 195 nm & excellent linearity
 WPI GmbH is designing and manufacturing proprietary equipment
for Original Equipment Manufacturers (OEMs) since 20 years
Mathias Belz et al. 30

31. World Precision Instruments
31Mathias Belz et al. Sample Cells (Cuvette & LWCC)
 Hersteller von Laborgeräten und optischer Messtechnik für die
Life Sciences seit 1967
 Niederlassungen in USA, Deutschland, UK, China & Brazilien
 Entwicklungszentrum für Optische Sensorik in Kooperation mit der
Technischen Hochschule Mittelhessen seit 2011
 2017 Umzug von Berlin nach Friedberg: Verkauf, Entwicklung und
Produktion
 Entwicklungfokus in Deutschland: Optische Flusszellen, LED-
Detektorsysteme, Fluorometer, optische Sensoren und Life
Science Applikationen
Questions send an email today info@wpiinc.com