Laboratory safety training

Programs for HIV Prevention and Treatment IRD UMI 174
Answers for Safety
Examination
Tassanee Lerksuthirat
PHPT-Nong Hoi 8 March 2010
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1. Who will be the next lab safety officer
head? (0.5 point)
The lucky person is WB
100% were correct!
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2. Please indicate the basic risk
management steps (also arrange the
steps)? (5 points) – only WB, PT, JK, PF,
AH, AT, UT and RD answer.
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2. Please indicate the basic risk
management steps (also arrange the
steps)? (5 points) – only WB, PT, JK, PF,
AH, AT, UT and RD answer.
?????
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3. In the risk assessment process, to
determine the level of risk, what factors
that we use to determine in PHPT lab? (2
points) – only WB, PT, JK, PF, AH, AT,
UT, and RD answer.
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4. According to the group health insurance
program, “all PHPT lab staff are covered
starting from the fourth month for life
insurance, accidental death and
disablement, total and permanent
disability by an accident, medical
payment (in and out-patient)” Is the
underlined sentence correct? (0.5 point)
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5. Which freezer is the serum/plasma
baseline for PHPT staff kept (at least, you
can draw the location of the freezer)? (1
points) – only WB, PT, AH, AT, UT and RD
answers.
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6. After immunization, why it is important
for the PHPT staff to keep the result of
anti-HBsAg testing in the personal file ?
(2 points)
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Start working Right after expose
or W/I 24 hrs.
3 months
After
expose
6 months
After
expose
12 months
After
expose
Patient ---> HIV-Positive
7. Why does the PHPT staff that being
exposed to the infectious material must
be collected the blood sample after the
incident? (3 points)
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7. Why does the PHPT staff that being
exposed to the infectious material must
be collected the blood sample after the
incident? (3 points)
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8. Would it be possible to use BSL-2 with the
pathogen that is classified as risk group
1? Why? (3 points)
• Microbiological risk gr. relate biosafety level (BSL)
but not equate to BSL
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9. Would if be possible to use BSC class I to
perform PBMC isolation, why (you don’t
give any reason, your score will be zero)?
(3 points)
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Biosafety Cabinet (BSC) Class I
A. Front opening
B. Work surface
C. Window
D. Exhaust plenum
E. HEPA filter
D.
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Biosafety Cabinet (BSC) Class II
A. Blower
B. Rear plenum
C. Supply HEPA filter
D. Exhaust HEPA filter
E. Sash
F. Work surface
D.
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10. What level of BSL dose PHPT lab currently
uses? From the first answer, would it be
possible to isolate pure HIV strain in our
lab? Why? (5 points)
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Biosafety Level (BSL)
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Biosafety Level (BSL): BSL-2
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?????
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BSL-1
Fume hood
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Standard Microbiological Practice
• Control access to laboratory
• Use mechanism pipetting devices
• Washing hands
• Prohibit eating, drinking & smoking
• Minimize splashes & aerosols
• Decontaminate work surfaces daily
• Decontaminate wastes
• Maintain insect & rodent control program
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BSL-2
BSC class I/II
Infectious trash
Biological
hazard sign
Autoclave
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BSL-3
BSC class I/II
Autoclave
Respirator
Restrict access
Swing double
door
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11. Please indicate each symbol refers to
what type of chemical? (5 points)
100% were correct!
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12. Which symbol from above (1-5) would
you label for Absolute ethanol? (1 point)
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13. According to the MSDS, what would you
do if the solution of ..... accidentally
splashes to your eye? (1 point)
100% were correct!
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21. According to the MSDS, what is the
major component that making the viral
lysis buffer can not be mix with Chlorox?
If we mix that component with Chlorox
what would happen? (only LL, JK, PD,
RD, PT, NW, SU, AH, TD, UT, PM, AA,WB
answer) (3 points)
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22. Please indicate each symbol (9 points)
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23. Please completely draw the fire escape
route (5 points)
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24. Can we use Bactyl as a sterilant? Why? (2
points)
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25.Since everyone generates infectious
waste, what does it mean if biological
indicator that currently use in PHPT lab
shows purple or yellow color after it has
been autoclaved and incubated for 48
hr. (everyone answers except PF and
Woratip)? (4 points)
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The End
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