Slides from Indus Instruments's webinar (http://goo.gl/vxypa8) on the critical need for vital signs monitoring during rodent surgery, and how the MouseMonitor™ S helps improve surgical consistency, documentation, and data recording. Including presentations from doctors Corey Reynolds from Baylor College of Medicine and Merry Lindsey from the University of Mississippi Medical Center.

2. InsideScientific is an online educational environment
designed for life science researchers.
Our goal is to aid in the sharing and distribution of scientific
information regarding innovative technologies, protocols,
research tools and laboratory services.

3. Merry Lindsey, PhD
Graham Sattler
Rodent surgical monitoring
advocate, applications
specialist, and Product
Manager for Indus
biomedical solutions
Director of the Jackson
Center for Heart Research at
The University of Mississippi,
celebrated professor, and
expert in murine models of
myocardial infarction
Corey Reynolds PhD
Director of the Mouse
Phenotyping Core Lab at
Baylor College of Medicine,
advanced murine model
surgeon, IACUC and animal
care expert

4. How do you currently document your surgery for method consistency?
Lab Notebook
Recording on Data
Acquisition System
Photos of Surgical
Setup
Strip Recording
0%
10%
20%
30%
40%
50%
60%
70%
80%

5. Surgical Monitoring Overview
What can Surgical Monitoring
do for Me?
MouseMonitor S Capabilities
Critical Surgical Monitoring
Signals
Integrated Surgical Monitoring
Doesn’t Have to Be Hard

6. What Can Surgical Monitoring Do For Me?
Increased
Survival Rate
Consistent
Procedures
Better Surgical
Training
Consistent
Outcomes

7. Surgical Monitoring Essentials…
Non-Invasive 6-Lead ECG
Blood Oxygenation
Monitoring
Respiration Monitoring
Responsive Heating
Core Temperature
Measurement

8. Critical Surgical Monitoring Signals
Multiple physiologic signals must be monitored to effectively assess subject health
Monitor…
Heart Rate
Core Temperature
Blood Oxygenation
Maintain…
Safe Core Temperature

9. Signal Integration…
…it doesn’t have to be hard
ECG, Heart Rate & Respiration Rate
from a single set of electrodes
Perform Surgeries directly on Stainless
Steel top
Intuitive Touch Screen Controls
Factory Presets Enable Out of Box
Operation
All Signals Captured Non-Invasively

10. Rodent Surgical Monitoring In Review
Comprehensive monitoring
offers greater consistency
and confidence
Consistent Interventions +
Higher Survival Rate = Better
Outcomes
The MouseMonitor S is the
only preclinical research
device that measures every
key vital sign in one device
Click Here For More Information

11. How important do you view rodent surgical monitoring?
Very Important
Important
Somewhat
Important
0%
10%
20%
30%
40%
50%
60%

12. What does the MPC Offer?
The Mouse Phenotyping Core is a comprehensive facility offering expertise in the following areas:
Imaging
Metabolic Assays
Cardiovasuclar Assays
Respiratory Assays
Neuromuscular Assessment
Surgical services

13. What does the MPC Offer?
MPC Cont.
Fee-for-service Core located at BCM
Can accept animals (rats/mice) from
outside facilities post veterinary approval
Can run long term studies and provide
data analysis
Contact Information: Corey L. Reynolds
713-798-5040--------Clreynol@bcm.edu

14. Challenges in a Core Lab…
Species
Clients/PIs
Many Different
Procedures
Protocols

15. Recommendations By The NRC
Guide for the Care and Use of Laboratory Animals , 8th ed.
Providing Artificial Heating (p119)
Monitoring baseline vital signs, including:
Heart Rate (p119)
Core Temperature (p119)
Blood Oxygenation (p122)

16. What a Core Lab Needs…
Core
temperature
monitoring &
adjustable
heating.
ECG/Heart Rate
electrode
flexibility for
different-sized
species.
Blood
Oxygenation
(SpO2)
monitoring.
Compact
Comprehensive
Surgical
monitoring.

17. Advantages of Integrated Monitoring & Recording
Easy Screenshots
Easy Recordings

18. Advantages of Integrated Monitoring & Recording
Constant
Monitoring of
Animal Vitals
During Surgery
Documentation
of Monitoring
Very Liked by
IACUC
committee.

19. Applications In The Mouse Phenotyping Core
Multiple Hi-Resolution
ECG leads enable easier
Morphology Recognition
& Arrhythmia Detection
Sinus Rhythm with Bigeminal PVCs

20. Applications In The Mouse Phenotyping Core
Serial Ultrasound (Vevo)
B-Mode and ECG capture
for analysis
ECG data integrates
effectively with other
equipment as well

21. What factors influence how frequently you utilize surgical monitoring?
My current setup takes too
much time to use
I am working on setting up
my lab
I don’t currently have
suitable monitoring
equipment
My current setup doesn’t
work properly
0%
5%
10%
15%
20%
25%
30%
35%

22. Myocardial Infarction (by Left Coronary Artery Decending (LAD) Ligation)C
Universal definition of myocardial infarction. Kristian Thygesen, Joseph S.
Alpert and Harvey D. White on behalf of the Joint
ESC/ACCF/AHA/WHF Task Force for the Redefinition of Myocardial Infarction.
Eur Heart J (2007) 28 (20): 2525-2538.

23. Post-MI Cardiac Remodeling
Yabluchanskiy et al. Congest Heart Fail. 2013

24. Lindsey Lab: MMPs & Impacts on Cardiac Remodeling

25. Lindsey Lab: MMPs & Impacts on Cardiac Remodeling

26. Lindsey Lab: MMPs & Impacts on Cardiac Remodeling

27. Lindsey Lab: MMPs & Impacts on Cardiac Remodeling

28. Lindsey Lab – Current Research Areas
Effects of MMP-9 deletion in aging mice post-MI
Effects of MMP-9 deletion post-MI in mice with P. gingivalis infection
Effects of MMPs derived peptides in vitro and in vivo
Effects of selective MMP inhibitors post-MI

29. ECG Before LAD Ligation

30. ECG 5 Min After LAD Ligation

31. Critical Needs in our Lab For In Vivo Models
Reliable & accurate ECG recording
Comfortable size of the pad
Multiple leads choice
External temperature probe
External and internal ECG electrodes
Portable tablet system
Controlled heated pad
Data management/export

32. Thank you for taking part in this event. We encourage all attendees to
register at www.insidescientific.com for notifications about future webinars.
WEBSERIES COMING SOON…
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