3. AIM OF PRESENTATION
To introduce the topic and
share my ideas with you
about Multiplex
Immunoassay and its
Methodology.
4. Sequence of Discussion
FINAL THOUGHT( CONCLUSION)
METHODOLOGY
PRINCIPLE
DIFINITION
INTRODUCTION OF MULTIPLEX IMMUNOASAY
5. INTRODUCTION OF MULTIPLEX IMMUNOASSAY
A Multiplex
assay is a
derivative
of an
“ELISA”
using beads
for binding
the capture
antibody.
The first
Immunoassay
was
developed by
“YALOW AND
BERSON” in
1960.
He also
received
Nobel prize
to measure
Insulin
level.
In 1970
“PETER
PERLMANN
AND EVA
ENGVALL”
published
paper that
synthesized
this
knowledge
into
method to
perform
“ELISA”.
The
Multiplex
assay is
based on
luminex’s
xMAP
technology.
6. Definition
Multiplex Assay is a type of
immunoassay that uses
magnetic beads to
simultaneously measure
multiple analyses in a single
experiment.
7. PRINCIPLE
In multiplex assay, microsphere (magnetic beads) are dyed to create
100 distinct colors.
Microsphere (magnetic beads) are coated with capture antibody.
Sample is added to microsphere (beads) and analyze is
captured.
Fluorescent tagged detected antibody is added.
Lasers detect both bead dyes and tagged detection antibody.
11. FINAL THOUGHT
CONCLUSION
• Multiplex immunoassay may also provide a wider
dynamic range than conventional ELISA.
• Multiplex system presents additional Quality control
challenges compared to uniplex analysis.
• Multiplex system allows a fast and systematic detection of
effects of micro molecules in different molecular and
cellular contexts.
• Multiplex system is capable of measuring potentially up to
100analytes simultaneously in a small sample volume.
