Shared Responsibilities & collaborative R&I Approaches – Ensuring safe & sustainable Products & Processes throughout complex Value-Chains MACRAMÉ’s Central Objective is to: • detect, characterise and quantify AdMas during handling and processing along the product life-cycle, • assess potential impacts on (human) health and the environment in intended or unintended exposure situations (i.e. ‘Exposure Points’) in the product value-chain, • advance the wide-spread applicability of the developed test and characterisation methods, by demonstrating their effectiveness and efficiency in the context of existing, market-relevant industrial AdMas containing products, and • prepare and initiate standardisation, harmonisation and technological & regulatory validation of test- and characterisation-methods. The MACRAMÉ Project is fully aligned with the EU ambitions to secure the safety and sustainability of new chemicals, materials, products and processes in order to strive for zero pollution and toxic-free environments, as addressed in the EU’s Chemical Strategy for Sustainability (2020), and in the European Green Deal (2021); in doing so, the Project concentrates on methodologies that are applicable to nanomaterials, and widens them to ‘Advanced Materials’ (AdMas) ⎼ a material category that includes but surpasses that of ‘nanomaterials’ (EU, ‘Definition of a Nanomaterial’) - in commercialised products and that are aligned with the future-oriented innovation, safety and sustainability considerations of the OECD (OECD (2020)), the EU (EU (2022)), and several of its Member States (e.g. Germany (2021)). This will be achieved through development and demonstration of novel methodologies, and by advancing their harmonisation & standardisation on three MACRAMÉ Material Families of inhalable carbon-based AdMas of various morphologies and dimensions (Tiwari et al. (2012)), beyond spherical particles: (a) graphene-related material (GRM), (b) carbon nanofibres (CNFs), e.g., carbon nanotubes (CNTs), and (c) Poly Lactic-co-Glycolic Acid (nano)particles (PLGA). The focus on carbon-based AdMas addresses unsolved detection and characterisation issues, especially in complex media. In doing so, MACRAMÉ builds on >15 years of research and innovation (R&I) and knowledge pooling in nanosafety, formed through numerous European and international collaborations. MACRAMÉ will add value to the results of collaborations, such as the Malta-Initiative, and the Graphene Flagship Validation Service and Standardisation Committee, to proactively support EU industries in becoming world-leaders in clean technologies and products and achieving the Green Deal’s ambitious timeline. This will be achieved by defining the R&I Strategy through life-cycle assessment for five market-relevant industrial MACRAMÉ Use-Cases. These define the selection of the MACRAMÉ R&I Activities and development of MACRAMÉ Methods, and the benchmarks chosen for monitoring the progress R&I.

1. The MACRAMÉ project has received funding from the European Union’s Horizon
Europe Research and Innovation programme under grant agreement No. 101092686.
Shared Responsibilities & collaborative R&I Approaches –
Ensuring safe & sustainable Products & Processes
throughout complex Value-Chains
EURONANOFORUM 2023
11TH – 13TH JUNE 2023

2. MACRAMÉ’s central Objectives
EuroNanoForum 2023
11th – 13th June 2023, Lund, Sweden
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• detect, characterise and quantify Advanced Materials (AdMas) during handling
and processing along the product life-cycle,
• assess potential impacts on (human) health and the environment in intended or
unintended exposure situations (i.e. ‘Exposure Points’) in the product value-
chain,
• advance the wide-spread applicability of the developed test and characterisation
methods, by demonstrating their effectiveness and efficiency in the context of
existing, market-relevant industrial AdMas containing products, and
• prepare and initiate standardisation, harmonisation and technological &
regulatory validation of test- and characterisation-methods.

3. The MACRAMÉ R&I Approach
3
Illustration of the MACRAMÉ R&I
Approach (AdMa@CMs: Advanced
Materials in complex matrices; CF:
Characterisation Factor; GRM:
graphene-related material; IATA:
integrated approaches to testing and
assessment; LCA: Life-Cycle
Assessment; LCC: Life-Cycle-Costing;
MFA: Material-Flow Analysis; RA:
Risk-Assessment; SSbD: Safe-&-
Sustainable-by-Design).
EuroNanoForum 2023
11th – 13th June 2023, Lund, Sweden
complex
Value-Chain
SSbD of
Products &
Processes
Shared
Respons-
ibility
Collaborative
R&I

4. market-relevant industrial MACRAMÉ Use-Cases:
• Graphene oxide (GO) flakes in drinking-water filters;
• Few-layer graphene (FLG) in battery management systems (BMS);
• Graphene-related materials (GRM) in car polish consumer sprays;
• Carbon nanotubes (i.e. CNTs) in car-seats; and
• Poly lactic-co-glycolic acid (PLGA) for inhalable antibiotics.
… selected to conduct:
• SSbD: Safe-&-Sustainable-by-Design
• LCA: Life-Cycle Assessment
• LCC: Life-Cycle-Costing
• MFA: Material-Flow Analysis
• RA: Risk-Assessment
… MACRAMÉ’s complex Value-Chains
4
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complex
Value-Chain

5. The MACRAMÉ’s Foundations
– Analyses, Assessments & Validations in industrial Value-Chains –
5
‘Wimmelbild’ (engl. ‘teeming picture’)
Pieter Bruegel d. Ä . (source:
© KHM-Museumsverband)
complex
Value-Chain
EuroNanoForum 2023
11th – 13th June 2023, Lund, Sweden

6. The MACRAMÉ’s Foundations
– Analyses, Assessments & Validations in industrial Value-Chains –
6
complex
Value-Chain
EuroNanoForum 2023
11th – 13th June 2023, Lund, Sweden

7. The MACRAMÉ’s Foundations
– Analyses, Assessments & Validations in industrial Value-Chains –
7
Terminology:
• AdMa = Advanced Material
• CM = Complex Matrix
• EM = Environmental Matrix
• BM = Biological Matrix
• CPM = Complex Product Matrix
• EoL = End-of-Life
• AdMa@CMs
• AdMa@EM
• AdMa@BM
• AdMa@EoL(CPM)
• AdMa@EoL(CPM)@EM
EuroNanoForum 2023
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complex
Value-Chain

8. The MACRAMÉ R&I Approach
8
Illustration of the MACRAMÉ R&I
Approach (AdMa@CMs: Advanced
Materials in complex matrices; CF:
Characterisation Factor; GRM:
graphene-related material; IATA:
integrated approaches to testing and
assessment; LCA: Life-Cycle
Assessment; LCC: Life-Cycle-Costing;
MFA: Material-Flow Analysis; RA:
Risk-Assessment; SSbD: Safe-&-
Sustainable-by-Design).
… Exposure Points …
EuroNanoForum 2023
11th – 13th June 2023, Lund, Sweden

9. The MACRAMÉ’s Foundations
– Analyses, Assessments & Validations in industrial Value-Chains –
9
Legend:
• AdMa = Advanced
Material
• CM = Complex Matrix
o EM = Environmental
Matrix
o BM = Biological Matrix
o CPM = Complex
Product Matrix
• EoL = End-of-Life
• AdMa@CMs
o AdMa@EM
o AdMa@BM
o AdMa@EoL(CPM)
o AdMa@EoL(CPM)@EM
EuroNanoForum 2023
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SSbD of
Products &
Processes

10. 10
Illustration of the MACRAMÉ R&I
Approach (AdMa@CMs: Advanced
Materials in complex matrices; CF:
Characterisation Factor; GRM:
graphene-related material; IATA:
integrated approaches to testing and
assessment; LCA: Life-Cycle
Assessment; LCC: Life-Cycle-Costing;
MFA: Material-Flow Analysis; RA:
Risk-Assessment; SSbD: Safe-&-
Sustainable-by-Design).
The MACRAMÉ R&I Approach … Characterisation & Testing …
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11. The MACRAMÉ Laboratory
– Characterisation & Testing of AdMas in complex Matrices –
11
In-vitro and ex-vivo Models that will be
assessed within the Project, indicating the
estimated cost that can be saved and MoA-
Information that can be obtained at the
different tiers.
Contact: Tommaso Serchi
(LIST)
Schematic illustration of the MACRAMÉ
ecotoxicity testing approach and outcomes.
Contact: Iseult Lynch (Uni Birmingham) & Alberto Katsumiti (GAIKER)
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SSbD of
Products &
Processes

12. The MACRAMÉ Processor
– Centralised Knowledge-Generation –
12
Schematic illustration of the flow- of and
value-add to data within the MACRAMÉ
Project.
Contact: Thomas Exner (7P9)
EuroNanoForum 2023
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Shared
Respons-
ibility
Collaborative
R&I

13. The MACRAMÉ Processor
– Centralised Knowledge-Generation –
13
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Shared
Respons-
ibility
Collaborative
R&I
In contrast to other depictions where FAIRification is a sixth
phase, FAIRification in MACRAMÉ is starting in the Plan &
Design phase and then continues throughout all other phases
continuously enhancing the FAIRness of the data (on-the-fly
FAIR).
Five phases of the data management life cycle.
Screenshot of an instance map
representing the planning status of
the central control material ordering
and shipping to the partners.

14. The MACRAMÉ Processor
– Centralised Knowledge-Generation –
14
Schematic illustration of the flow- of and
value-add to data within the MACRAMÉ
Project.
Contact: Thomas Exner (7P9)
*** read the full article ***
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Shared
Respons-
ibility
Collaborative
R&I

15. The MACRAMÉ Team
15
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Collaborative
R&I

16. The MACRAMÉ Team
16
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Partners
19
Collaborative
R&I
Shared
Respons-
ibility
Collaborative
R&I
5
… sampled from
market-relevant industrial MACRAMÉ Use-Cases
10
… from
Countries
3
… characterising and testing
Material Families of inhalable carbon-based AdMas

17. The MACRAMÉ project has received funding from the European Union’s Horizon
Europe Research and Innovation programme under grant agreement No. 101092686.
Thank you
www.macrame-project.eu
Steffi Friedrichs
Steffi@AcumenIST.com
+32 496 075 785