hybrid seed production in Rice ELP program

1. College of Agriculture,
Balaghat
Final Presentation
On
Experiential Learning Programme
Hybrid Seed Production Technology
Submitted To,
Dr. N.K. Bisen
(Associate Professor) & (Managing
Director)
Dr. Uttam Bisen
Submitted By,
Shahnul
B.Sc. (Ag.) 4th Year, 2nd Sem.
Enrollment No.- 180401057

2. History of Hybrid Rice Technology in World and India
In 1976 - First commercial three-line rice hybrid released in China.
In 1994 first time hybrid rice (APHR1, APHR2, MGR1 and KRH1) were released in
India.
India has released 96 rice hybrids

3. Hybrid JRH-19
• Parents:
Female line - IR58056 A
Male line - NPT 13-01
• Extra-Early maturing hybrid: 100-105
days
• Yield : 60-65 q /ha
• Grains are medium slender.
• Drought tolerant
• Suitable for double cropping (Rice-Gram)
under rainfed condition

4. Selection of field
Farmers have self irrigation facilities
• Not dependent upon channels, Tanks, Rivers etc.
• Successful Tube wells, and Wells
• Availability of electricity
Favorable climatic conditions
• Sufficient sunshine- Good for photosynthesis
• Moderate wind velocity- No lodging
• No rainfall during peak flowering season
Low incidence of pest and diseases
• Sometimes stem borer and blast are seen.

5.  Temperature do not go below 8-12oC &
 No frost conditions
 Day temperature - 24-32oC
 Night temperature does not go below 20oC
 Day temperature - 32-37oC
 Night temperature does not go below 25oC
Nursery & Initial
Growing stages
Last Forth night of
November
&
December-January
February-March
First Forth night of
April
Vegetative
stage
Last Forth night of
April
First Forth Night of
May
Flowering &
Dough stage

6. CGMS System (3-Line System for Hybrid Seed Production):
 In India, CGMS system is being used for hybrid rice seed production at a
commercial scale.
 A-line = female(sterile)
 B-line =maintainer
 R-line = Restorer
Hybrid seed production using the CGMS System involves the two
following steps-
 Production of A-line (A × B)
Production of Hybrid Seed (A × R)

7. Seed Parent
A-Line
Maintainer
B-Line
X
A-line
Produces unviable pollen grains
Seed Parent
A-Line
Pollen Parent
R-Line
X
F1-Hybrid
Produces viable pollen and act as
seeds which are used to plant
commercial rice crop

8. Isolation for Hybrid Seed Production
➢Space Isolation – 100m isolation required from other
variety.
➢Time isolation – 21 days would be effective
➢Barrier Isolation – Artificial barrier of 2m height is
also used.
➢ Border row - Planting extra R line
Requirement of seed and seed rate
Seed of A line Female parent 15 kg/ha
Seed of R line Male parent 5-7kg/ha

9. 5. Sowing of Seed in the Nursery-
Time of Sowing of seed of A and R line depends on time of flowering for Hybrid Seed.
Both parents should flower at same time, this is known as NICKING. By STAGGERING.
S. No. Seed/Pollen Parent Seeding
sequence
1. A-line 0 day
2. First R-line 7th day
3. Second R-line 13th day
4. Third R-line 19th day
Staggered sowing of parents for flowering synchronization

10. 8. Transplanting
Field Layout
R line A line R Line
X
X
X
X
X
X
X
X
X
X
X
X
0 0 0 0 0 0
0 0 0 0 0 0
0 0 0 0 0 0
0 0 0 0 0 0
0 0 0 0 0 0
0 0 0 0 0 0
X
X
X
X
X
X
X
X
X
X
X
X
15 cm 15 cm 30 cm
15 cm
Spacing and Field Layout:
Between the Block of A & R line – 30cm
Between Rows of R-line – 15cm
Between Rows of A-line –15 cm
Between hills/plants –15cm
Row Ratio R:A- 2:6

12. 9. After-Management
Application of Urea + DAP between R-line Rows

13. 10. Rouging
Removal undesirable rice plants ,off-
types , volunteer plants & Pollen
shedders is necessary to maintain
Genetic Purity hybrid seed.
The most important stages for rouging
are at maximum tillering, at flowering
and just before harvesting
Based on
plant height,
plant type,
Out side of row
sheath colour,
Morphological basis of Rouging

15. 11. Observation of Primordial Growth (Panicle Development)
Primordial growth in parents is observed in between after transplanting and matched with
female parent to predict flowering of male and female parent:-
 For this select the main tiller and cut it from the base where stem and root join.
Make a longitudinal slit at the base up to top of the tiller.
Open the tiller just above the nodal portion.
 Observe the developing panicle and match development of male and female parent
12. Synchronization of Flowering Date
In the case of mismatch of male and female parents following treatment may be applied:
•Propone – can be done by foliar spray of 2 % solution of SSP before flowering in later
flowering parent.
•Postpone – can be done by foliar spray of 2% solution of Urea before flowering in early
flowering parent.

17. 13. Application of Gibberellic acid (GA3)
 Gibberellic acid is applied for complete exertion of panicle.
 20 gm GA3 powder dissolved in 200ml surgical spirit for 0.2 hectare.area.
 3 split applications: (Alternate Days)
 1st - Common spray at the time of 10-15 % natural flowering has taken
place @ 100ml GA3 solution in 100 liter water for 0.2 hectare
 2nd -After one day GA3 solution @ 40ml in 100 liter water on male line.
 3rd -After two days GA3 solution @ 60ml in 100 liter water on female line.

19. 14. Supplementary Pollination
It is a technique of shaking pollen parent so that pollen shed and effectively dispersed
over A- line plants.
It can be either done by rope pulling or by shaking pollen parent by bamboo sticks.
This process is repeated 3-4 times during day at interval of 30 minutes from 8.00-
11.00 AM daily.
Supplementary Pollination has to be done for 7-10 days during the flowering period.

20. 15. Harvesting, Threshing & Yield
Harvest R-line rows first. Remove harvested R-line and keep it separately.
Carefully remove R -line panicles from field. Then harvest A-line plants..
Before starting threshing all the threshing equipment, threshing floor, and
tarpaulin to be thoroughly cleaned. A & R line should threshed separately.
Yield- 4 quintal seed yield and 3 quintal R-line grain is estimated from
0.2 hectare area

22. 16. Economic Analysis of Hybrid Rice Seed Production

23. THANK YOU